Int. J. Mol. Sci. 2011, 12(11), 7352-7359; doi:10.3390/ijms12117352
Article

Antiproliferative Effect of Aaptamine on Human Chronic Myeloid Leukemia K562 Cells

1email, 1email, 1email, 2email, 1,* email and 1,* email
Received: 26 September 2011; in revised form: 18 October 2011 / Accepted: 20 October 2011 / Published: 26 October 2011
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: We previously isolated aaptamine, a benzonaphthyridine alkaloid, from marine sponge Aaptos suberitoids. In this study, we investigated the anti-proliferative effect of aaptamine on chronic myeloid leukemia (CML) K562 cells. Aaptamine inhibited growth of K562 with a GI50 as 10 μM, and arrested cell cycle at G2/M phase. Western blot analysis indicated that aaptamine induced p21 expression in K562 cells. Moreover, p21 promoter was activated by aaptamine treatment in p21 transfected K562 cells. Since K562 is p53 negative, aaptamine was demonstrated to be a p53-independent p21 inducer in CML cells.
Keywords: aaptamine; K562 cells; G2/M arrest; p21
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MDPI and ACS Style

Jin, M.; Zhao, W.; Zhang, Y.; Kobayashi, M.; Duan, H.; Kong, D. Antiproliferative Effect of Aaptamine on Human Chronic Myeloid Leukemia K562 Cells. Int. J. Mol. Sci. 2011, 12, 7352-7359.

AMA Style

Jin M, Zhao W, Zhang Y, Kobayashi M, Duan H, Kong D. Antiproliferative Effect of Aaptamine on Human Chronic Myeloid Leukemia K562 Cells. International Journal of Molecular Sciences. 2011; 12(11):7352-7359.

Chicago/Turabian Style

Jin, Meihua; Zhao, Wennan; Zhang, Yanwen; Kobayashi, Motomasa; Duan, Hongquan; Kong, Dexin. 2011. "Antiproliferative Effect of Aaptamine on Human Chronic Myeloid Leukemia K562 Cells." Int. J. Mol. Sci. 12, no. 11: 7352-7359.


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