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Int. J. Mol. Sci. 2011, 12(11), 7352-7359; doi:10.3390/ijms12117352

Antiproliferative Effect of Aaptamine on Human Chronic Myeloid Leukemia K562 Cells

1,*  and 1,*
1 Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics, School of Pharmaceutical Sciences and Research Center of Basic Medical Sciences, Tianjin Medical University, Tianjin 300070, China 2 Graduate School of Pharmaceutical Sciences, Osaka University, Yamada-oka 1-6, Suita, Osaka 565-0871, Japan
* Authors to whom correspondence should be addressed.
Received: 26 September 2011 / Revised: 18 October 2011 / Accepted: 20 October 2011 / Published: 26 October 2011
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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We previously isolated aaptamine, a benzonaphthyridine alkaloid, from marine sponge Aaptos suberitoids. In this study, we investigated the anti-proliferative effect of aaptamine on chronic myeloid leukemia (CML) K562 cells. Aaptamine inhibited growth of K562 with a GI50 as 10 μM, and arrested cell cycle at G2/M phase. Western blot analysis indicated that aaptamine induced p21 expression in K562 cells. Moreover, p21 promoter was activated by aaptamine treatment in p21 transfected K562 cells. Since K562 is p53 negative, aaptamine was demonstrated to be a p53-independent p21 inducer in CML cells.
Keywords: aaptamine; K562 cells; G2/M arrest; p21 aaptamine; K562 cells; G2/M arrest; p21
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Jin, M.; Zhao, W.; Zhang, Y.; Kobayashi, M.; Duan, H.; Kong, D. Antiproliferative Effect of Aaptamine on Human Chronic Myeloid Leukemia K562 Cells. Int. J. Mol. Sci. 2011, 12, 7352-7359.

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