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Int. J. Mol. Sci. 2011, 12(11), 7301-7313; doi:10.3390/ijms12117301

A Germin-Like Protein Gene (CchGLP) of Capsicum chinense Jacq. Is Induced during Incompatible Interactions and Displays Mn-Superoxide Dismutase Activity

1 División de Ciencias de la Vida, Departamento de Ingeniería en Alimentos, Campus Irapuato-Salamanca, Universidad de Guanajuato, Ex-Hacienda el Copal km 9, Carretera Irapuato-Silao, CP 36500, Irapuato Guanajuato, Mexico 2 División de Biología Molecular, Instituto Potosino de Investigación Científica y Tecnológica, Camino a la Presa San José 2055, Colima, Lomas 4 sección CP 78216, San Luis Potosí, S.L.P. Mexico 3 Laboratorio de Biología Molecular, Departamento de Ingeniería Bioquímica, Instituto Tecnológico de Celaya, Avenida Tecnológico y A, García-Cubas, S/N, Col, FOVISSSTE, CP 38010, Celaya, Guanajuato, Mexico 4 C.A de Ingeniería de Biosistemas, Facultad de Ingeniería, Universidad Autónoma de Querétaro, Centro Universitario Cerro de las Campanas, S/N, Col, Las Campanas, CP 76010, Santiago de Querétaro, Querétaro, Mexico 5 Unidad de Biotecnología, Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias, Carr, Celaya-San Miguel de Allende, km 6, CP 38010, Celaya, Guanajuato, Mexico
* Author to whom correspondence should be addressed.
Received: 21 July 2011 / Revised: 4 September 2011 / Accepted: 17 October 2011 / Published: 25 October 2011
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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A germin-like gene (CchGLP) cloned from geminivirus-resistant pepper (Capsicum chinense Jacq. Line BG-3821) was characterized and the enzymatic activity of the expressed protein analyzed. The predicted protein consists of 203 amino acids, similar to other germin-like proteins. A highly conserved cupin domain and typical germin boxes, one of them containing three histidines and one glutamate, are also present in CchGLP. A signal peptide was predicted in the first 18 N-terminal amino acids, as well as one putative N-glycosylation site from residues 44–47. CchGLP was expressed in E. coli and the recombinant protein displayed manganese superoxide dismutase (Mn-SOD) activity. Molecular analysis showed that CchGLP is present in one copy in the C. chinense Jacq. genome and was induced in plants by ethylene (Et) and salicylic acid (SA) but not jasmonic acid (JA) applications in the absence of pathogens. Meanwhile, incompatible interactions with either Pepper golden mosaic virus (PepGMV) or Pepper huasteco yellow vein virus (PHYVV) caused local and systemic CchGLP induction in these geminivirus-resistant plants, but not in a susceptible accession. Compatible interactions with PHYVV, PepGMV and oomycete Phytophthora capsici did not induce CchGLP expression. Thus, these results indicate that CchGLP encodes a Mn-SOD, which is induced in the C. chinense geminivirus-resistant line BG-3821, likely using SA and Et signaling pathways during incompatible interactions with geminiviruses PepGMV and PHYVV.
Keywords: GLP; geminiviruses; Mn-SOD; ethylene; salicylic acid GLP; geminiviruses; Mn-SOD; ethylene; salicylic acid
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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León-Galván, F.; Joaquín-Ramos, A.J.; Torres-Pacheco, I.; de la Rosa, A.P.B.; Guevara-Olvera, L.; González-Chavira, M.M.; Ocampo-Velazquez, R.V.; Rico-García, E.; Guevara-González, R.G. A Germin-Like Protein Gene (CchGLP) of Capsicum chinense Jacq. Is Induced during Incompatible Interactions and Displays Mn-Superoxide Dismutase Activity. Int. J. Mol. Sci. 2011, 12, 7301-7313.

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