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Modulation of Ca2+ Signals by Epigallocatechin-3-gallate(EGCG) in Cultured Rat Hippocampal Neurons
College of Fisheries, Huazhong Agricultural University, Wuhan 430070, Hubei, China
Department of Pharmacy, The Affiliated Xiangfan Hospital of TongJi Medical College of Huazhong University of Science & Technology, Xiangfan 441021, Hubei, China
Department of Pharmacology, Medical College, Xianning University, Xianning 437100, Hubei, China
Department of Osteopaedics, Center Hospital of Xianning, Xianning 437100, Hubei, China
* Author to whom correspondence should be addressed.
Received: 22 December 2010; in revised form: 30 December 2010 / Accepted: 4 January 2011 / Published: 20 January 2011
Abstract: Green tea has been receiving considerable attention as a possible neuroprotective agent against neurodegenerative disease. Epigallocatechin-3-gallate (EGCG) is the major compound of green tea. Calcium signaling has profound effects on almost all aspects of neuronal function. Using digital calcium imaging and patch-clamp technique, we determined the effects of EGCG on Ca2+ signals in hippocampal neurons. The results indicated that EGCG caused a dose-dependent increase in intracellular Ca2+ ([Ca2+]i). This [Ca2+]i increase was blocked by depleting intracellular Ca2+ stores with the endoplasmic reticulum Ca2+ pump inhibitor thapsigargin and cyclopiazonic acid. Furthermore, EGCG-stimulated increase in [Ca2+]i was abolished following treatment with a PLC inhibitor. However, EGCG inhibited high-voltage activated Ca2+ currents (IHVA) and NMDA-induced inward currents (INMDA). These data suggest that EGCG triggers a cascade of events: it activates phospholipase C (PLC), mobilizes intracellular Ca2+ stores, raises the cytosolic Ca2+ levels, and inhibits the VGCC and NMDA receptors-mediated Ca2+ influx through a process that remains to be determined.
Keywords: EGCG; calcium imaging; intracellular Ca2+; phopholipase C; hippocampal neuron
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Wang, J.-H.; Cheng, J.; Li, C.-R.; Ye, M.; Ma, Z.; Cai, F. Modulation of Ca2+ Signals by Epigallocatechin-3-gallate(EGCG) in Cultured Rat Hippocampal Neurons. Int. J. Mol. Sci. 2011, 12, 742-754.
Wang J-H, Cheng J, Li C-R, Ye M, Ma Z, Cai F. Modulation of Ca2+ Signals by Epigallocatechin-3-gallate(EGCG) in Cultured Rat Hippocampal Neurons. International Journal of Molecular Sciences. 2011; 12(1):742-754.
Wang, Jiang-Hua; Cheng, Jin; Li, Cai-Rong; Ye, Mao; Ma, Zhe; Cai, Fei. 2011. "Modulation of Ca2+ Signals by Epigallocatechin-3-gallate(EGCG) in Cultured Rat Hippocampal Neurons." Int. J. Mol. Sci. 12, no. 1: 742-754.