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Int. J. Mol. Sci. 2011, 12(1), 742-754; doi:10.3390/ijms12010742

Modulation of Ca2+ Signals by Epigallocatechin-3-gallate(EGCG) in Cultured Rat Hippocampal Neurons

1 College of Fisheries, Huazhong Agricultural University, Wuhan 430070, Hubei, China 2 Department of Pharmacy, The Affiliated Xiangfan Hospital of TongJi Medical College of Huazhong University of Science & Technology, Xiangfan 441021, Hubei, China 3 Department of Pharmacology, Medical College, Xianning University, Xianning 437100, Hubei, China 4 Department of Osteopaedics, Center Hospital of Xianning, Xianning 437100, Hubei, China
* Author to whom correspondence should be addressed.
Received: 22 December 2010 / Revised: 30 December 2010 / Accepted: 4 January 2011 / Published: 20 January 2011
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Green tea has been receiving considerable attention as a possible neuroprotective agent against neurodegenerative disease. Epigallocatechin-3-gallate (EGCG) is the major compound of green tea. Calcium signaling has profound effects on almost all aspects of neuronal function. Using digital calcium imaging and patch-clamp technique, we determined the effects of EGCG on Ca2+ signals in hippocampal neurons. The results indicated that EGCG caused a dose-dependent increase in intracellular Ca2+ ([Ca2+]i). This [Ca2+]i increase was blocked by depleting intracellular Ca2+ stores with the endoplasmic reticulum Ca2+ pump inhibitor thapsigargin and cyclopiazonic acid. Furthermore, EGCG-stimulated increase in [Ca2+]i was abolished following treatment with a PLC inhibitor. However, EGCG inhibited high-voltage activated Ca2+ currents (IHVA) and NMDA-induced inward currents (INMDA). These data suggest that EGCG triggers a cascade of events: it activates phospholipase C (PLC), mobilizes intracellular Ca2+ stores, raises the cytosolic Ca2+ levels, and inhibits the VGCC and NMDA receptors-mediated Ca2+ influx through a process that remains to be determined.
Keywords: EGCG; calcium imaging; intracellular Ca2+; phopholipase C; hippocampal neuron EGCG; calcium imaging; intracellular Ca2+; phopholipase C; hippocampal neuron
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Wang, J.-H.; Cheng, J.; Li, C.-R.; Ye, M.; Ma, Z.; Cai, F. Modulation of Ca2+ Signals by Epigallocatechin-3-gallate(EGCG) in Cultured Rat Hippocampal Neurons. Int. J. Mol. Sci. 2011, 12, 742-754.

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