Modulation of Ca2+ Signals by Epigallocatechin-3-gallate(EGCG) in Cultured Rat Hippocampal Neurons
AbstractGreen tea has been receiving considerable attention as a possible neuroprotective agent against neurodegenerative disease. Epigallocatechin-3-gallate (EGCG) is the major compound of green tea. Calcium signaling has profound effects on almost all aspects of neuronal function. Using digital calcium imaging and patch-clamp technique, we determined the effects of EGCG on Ca2+ signals in hippocampal neurons. The results indicated that EGCG caused a dose-dependent increase in intracellular Ca2+ ([Ca2+]i). This [Ca2+]i increase was blocked by depleting intracellular Ca2+ stores with the endoplasmic reticulum Ca2+ pump inhibitor thapsigargin and cyclopiazonic acid. Furthermore, EGCG-stimulated increase in [Ca2+]i was abolished following treatment with a PLC inhibitor. However, EGCG inhibited high-voltage activated Ca2+ currents (IHVA) and NMDA-induced inward currents (INMDA). These data suggest that EGCG triggers a cascade of events: it activates phospholipase C (PLC), mobilizes intracellular Ca2+ stores, raises the cytosolic Ca2+ levels, and inhibits the VGCC and NMDA receptors-mediated Ca2+ influx through a process that remains to be determined.
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Wang, J.-H.; Cheng, J.; Li, C.-R.; Ye, M.; Ma, Z.; Cai, F. Modulation of Ca2+ Signals by Epigallocatechin-3-gallate(EGCG) in Cultured Rat Hippocampal Neurons. Int. J. Mol. Sci. 2011, 12, 742-754.
Wang J-H, Cheng J, Li C-R, Ye M, Ma Z, Cai F. Modulation of Ca2+ Signals by Epigallocatechin-3-gallate(EGCG) in Cultured Rat Hippocampal Neurons. International Journal of Molecular Sciences. 2011; 12(1):742-754.Chicago/Turabian Style
Wang, Jiang-Hua; Cheng, Jin; Li, Cai-Rong; Ye, Mao; Ma, Zhe; Cai, Fei. 2011. "Modulation of Ca2+ Signals by Epigallocatechin-3-gallate(EGCG) in Cultured Rat Hippocampal Neurons." Int. J. Mol. Sci. 12, no. 1: 742-754.