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Communication
Sixteen Polymorphic Simple Sequence Repeat Markers from Expressed Sequence Tags of the Chinese Mitten Crab Eriocheir sinensis
1
Liaoning Key Laboratory of Marine Fishery Molecular Biology, Liaoning Ocean and Fishery Science Institute, 50 Heishijiao St, Dalian, Liaoning 116023, China
2
College of Life Science, Liaoning Normal University, 100 Huanghe Rd. Dalian, Liaoning 116029, China
3
College of Life Sciences and Biotechnology, Dalian Ocean University, 52 Heishijiao St, Dalian, Liaoning 116023, China
* Author to whom correspondence should be addressed.
Received: 23 June 2010; in revised form: 17 August 2010 / Accepted: 18 August 2010 / Published: 24 August 2010
Abstract: The Chinese mitten crab (Eriocheir sinensis) is an economically important aquaculture species in China. In this study, we developed and evaluated simple sequence repeat markers from expressed sequence tags of E. sinensis. Among the 40 wild E. sinensis individuals tested, 16 loci were polymorphic. The number of alleles per locus ranged from two to ten. The observed heterozygosity ranged from 0.0667 to 0.9667, whereas the expected heterozygosity ranged from 0.0661 to 0.9051. These markers have the potential for use in genetic studies of population structure and intraspecific variation in E. sinensis.
Keywords: Chinese mitten crab; Eriocheir sinensis; expressed sequence tags; simple sequence repeats
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Cite This Article
MDPI and ACS Style
Gao, X.-G.; Li, H.-J.; Li, Y.-F.; Sui, L.-J.; Zhu, B.; Liang, Y.; Liu, W.-D.; He, C.-B. Sixteen Polymorphic Simple Sequence Repeat Markers from Expressed Sequence Tags of the Chinese Mitten Crab Eriocheir sinensis. Int. J. Mol. Sci. 2010, 11, 3035-3038.
AMA Style
Gao X-G, Li H-J, Li Y-F, Sui L-J, Zhu B, Liang Y, Liu W-D, He C-B. Sixteen Polymorphic Simple Sequence Repeat Markers from Expressed Sequence Tags of the Chinese Mitten Crab Eriocheir sinensis. International Journal of Molecular Sciences. 2010; 11(8):3035-3038.
Chicago/Turabian Style
Gao, Xiang-Gang; Li, Hong-Jun; Li, Yun-Feng; Sui, Li-Jun; Zhu, Bao; Liang, Yu; Liu, Wei-Dong; He, Chong-Bo. 2010. "Sixteen Polymorphic Simple Sequence Repeat Markers from Expressed Sequence Tags of the Chinese Mitten Crab Eriocheir sinensis." Int. J. Mol. Sci. 11, no. 8: 3035-3038.