Int. J. Mol. Sci. 2010, 11(8), 2856-2863; doi:10.3390/ijms11082856
Article

Reactivation of Human Acetylcholinesterase and Butyrylcholinesterase Inhibited by Leptophos-Oxon with Different Oxime Reactivators in Vitro

1 Faculty of Military Health Sciences, University of Defence, Hradec Kralove, Czech Republic 2 Faculty of Environmental Sciences, Czech University of Life Sciences Prague, Praha, Czech Republic 3 Faculty of Pharmacy in Hradec Kralove, Charles University in Prague, Hradec Kralove, Czech Republic 4 Medicinal Science Division, Korea Research Institute of Chemical Technology, Yuseong, Taejon, Korea
* Authors to whom correspondence should be addressed.
Received: 29 June 2010; in revised form: 25 July 2010 / Accepted: 29 July 2010 / Published: 3 August 2010
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
PDF Full-text Download PDF Full-Text [105 KB, uploaded 3 August 2010 11:31 CEST]
Abstract: We have evaluated in vitro the potency of 23 oximes to reactivate human erythrocyte acetylcholinesterase (AChE) and plasma butyrylcholinesterase (BChE) inhibited by racemic leptophos-oxon (O-[4-bromo-2,5-dichlorophenyl]-O-methyl phenyl-phosphonate), a toxic metabolite of the pesticide leptophos. Compounds were assayed in concentrations of 10 and 100 µM. In case of leptophos-oxon inhibited AChE, the best reactivation potency was achieved with methoxime, trimedoxime, obidoxime and oxime K027. The most potent reactivators of inhibited BChE were K033, obidoxime, K117, bis-3-PA, K075, K074 and K127. The reactivation efficacy of tested oximes was lower in case of leptophos-oxon inhibited BChE.
Keywords: acetylcholinesterase; butyrylcholinesterase; nerve agent; pesticide; reactivator; oxime; scavenger; leptophos-oxon

Article Statistics

Load and display the download statistics.

Citations to this Article

Cite This Article

MDPI and ACS Style

Jun, D.; Musilova, L.; Pohanka, M.; Jung, Y.-S.; Bostik, P.; Kuca, K. Reactivation of Human Acetylcholinesterase and Butyrylcholinesterase Inhibited by Leptophos-Oxon with Different Oxime Reactivators in Vitro. Int. J. Mol. Sci. 2010, 11, 2856-2863.

AMA Style

Jun D, Musilova L, Pohanka M, Jung Y-S, Bostik P, Kuca K. Reactivation of Human Acetylcholinesterase and Butyrylcholinesterase Inhibited by Leptophos-Oxon with Different Oxime Reactivators in Vitro. International Journal of Molecular Sciences. 2010; 11(8):2856-2863.

Chicago/Turabian Style

Jun, Daniel; Musilova, Lucie; Pohanka, Miroslav; Jung, Young-Sik; Bostik, Pavel; Kuca, Kamil. 2010. "Reactivation of Human Acetylcholinesterase and Butyrylcholinesterase Inhibited by Leptophos-Oxon with Different Oxime Reactivators in Vitro." Int. J. Mol. Sci. 11, no. 8: 2856-2863.

Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert