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Int. J. Mol. Sci. 2010, 11(5), 1991-1998; doi:10.3390/ijms11051991

Synthesis of 4′,7-Diacetoxyapigenin and Its Apoptotic Induction in Human Hep G2 Cells

1,* , 2
1 College of Light Industry and Food Science, South China University of Technology, Guangzhou 510640, China 2 The Key Laboratory of Chemical Biology, Guangdong Province, Graduate School at Shenzhen, Tsinghua University, Guangdong 518055, China 3 School of Food Science, Henan Institute of Science and Technology, Xinxiang 453003, China
* Author to whom correspondence should be addressed.
Received: 30 March 2010 / Accepted: 23 April 2010 / Published: 30 April 2010
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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In this study, 4′,7-diacetoxyapigenin [4-(7-acetoxy-5-hydroxy-4-oxo-4H-chromen-2-yl) phenyl acetate] was synthesized for the first time. Its chemical structure was identified by UV, ESI-MS, 1H and 13C-NMR. It could inhibit the proliferation of Hep G2 cells in a dose-dependent manner and induce the significant increase of the G0/G1 cell population. After treatment by 4′,7-diacetoxyapigenin, phosphatidylserine of Hep G2 cells could significantly translocate to the surface of the membrane. The increase of an early apoptotic population was observed by both annexin-FITC and PI staining. It was concluded that 4′,7-diacetoxyapigenin not only induced cells to enter into apoptosis, but also affected the progress of the cell cycle.
Keywords: apoptosis; cell cycle; Hep G2; 4′,7-diacetoxyapigenin apoptosis; cell cycle; Hep G2; 4′,7-diacetoxyapigenin
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Xu, K.; Liu, F.; Liu, B.; Gao, H.; Ning, Z. Synthesis of 4′,7-Diacetoxyapigenin and Its Apoptotic Induction in Human Hep G2 Cells. Int. J. Mol. Sci. 2010, 11, 1991-1998.

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