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Synthesis of 4′,7-Diacetoxyapigenin and Its Apoptotic Induction in Human Hep G2 Cells
College of Light Industry and Food Science, South China University of Technology, Guangzhou 510640, China
The Key Laboratory of Chemical Biology, Guangdong Province, Graduate School at Shenzhen, Tsinghua University, Guangdong 518055, China
School of Food Science, Henan Institute of Science and Technology, Xinxiang 453003, China
* Author to whom correspondence should be addressed.
Received: 30 March 2010; Accepted: 23 April 2010 / Published: 30 April 2010
Abstract: In this study, 4′,7-diacetoxyapigenin [4-(7-acetoxy-5-hydroxy-4-oxo-4H-chromen-2-yl) phenyl acetate] was synthesized for the first time. Its chemical structure was identified by UV, ESI-MS, 1H and 13C-NMR. It could inhibit the proliferation of Hep G2 cells in a dose-dependent manner and induce the significant increase of the G0/G1 cell population. After treatment by 4′,7-diacetoxyapigenin, phosphatidylserine of Hep G2 cells could significantly translocate to the surface of the membrane. The increase of an early apoptotic population was observed by both annexin-FITC and PI staining. It was concluded that 4′,7-diacetoxyapigenin not only induced cells to enter into apoptosis, but also affected the progress of the cell cycle.
Keywords: apoptosis; cell cycle; Hep G2; 4′,7-diacetoxyapigenin
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Xu, K.; Liu, F.; Liu, B.; Gao, H.; Ning, Z. Synthesis of 4′,7-Diacetoxyapigenin and Its Apoptotic Induction in Human Hep G2 Cells. Int. J. Mol. Sci. 2010, 11, 1991-1998.
Xu K, Liu F, Liu B, Gao H, Ning Z. Synthesis of 4′,7-Diacetoxyapigenin and Its Apoptotic Induction in Human Hep G2 Cells. International Journal of Molecular Sciences. 2010; 11(5):1991-1998.
Xu, Keyong; Liu, Feng; Liu, Benguo; Gao, Han; Ning, Zhengxiang. 2010. "Synthesis of 4′,7-Diacetoxyapigenin and Its Apoptotic Induction in Human Hep G2 Cells." Int. J. Mol. Sci. 11, no. 5: 1991-1998.