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Int. J. Mol. Sci. 2010, 11(4), 1944-1955; doi:10.3390/ijms11041944
Article

Effects of Aflatoxin B1 and Fumonisin B1 on the Viability and Induction of Apoptosis in Rat Primary Hepatocytes

1, 2, 2, 1 and 2,*
1 Centro de P&D de Proteção Ambiental, Instituto Biológico, Av. Conselheiro Rodrigues Alves n. 1252 – Vila Mariana. São Paulo, SP. 04014-002, Brazil 2 Departamento de Microbiologia, Instituto de Ciências Biomédicas – Universidade de São Paulo, Av. Prof. Lineu Prestes n. 1374 – Cidade Universitária. São Paulo, SP. 05508-900, Brazil
* Author to whom correspondence should be addressed.
Received: 23 February 2010 / Revised: 17 March 2010 / Accepted: 29 March 2010 / Published: 26 April 2010
(This article belongs to the Section Bioinorganic Chemistry)
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Abstract

The present study evaluated the effect of aflatoxin B1 (AFB1) and fumonisin B1 (FB1) either alone, or in association, on rat primary hepatocyte cultures. Cell viability was assessed by flow cytometry after propidium iodine intercalation. DNA fragmentation and apoptosis were assessed by agarose gel electrophoresis and acridine orange and ethidium bromide staining. At the concentrations of AFB1 and FB1 used, the toxins did not decrease cell viability, but did induce apoptosis in a concentration and time-dependent manner.
Keywords: aflatoxin B1; apoptosis; fumonisin B1; primary hepatocytes aflatoxin B1; apoptosis; fumonisin B1; primary hepatocytes
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Ribeiro, D.H.B.; Ferreira, F.L.; Da Silva, V.N.; Aquino, S.; Corrêa, B. Effects of Aflatoxin B1 and Fumonisin B1 on the Viability and Induction of Apoptosis in Rat Primary Hepatocytes. Int. J. Mol. Sci. 2010, 11, 1944-1955.

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