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Extraction of Biomolecules Using Phosphonium-Based Ionic Liquids + K3PO4 Aqueous Biphasic Systems
Departamento de Química, CICECO, Universidade de Aveiro, 3810-193 Aveiro, Portugal
Instituto de Tecnologia Química e Biológica, UNL, Av. República, Ap. 127, 2780-901 Oeiras, Portugal
Laboratoire Haute Pression Centre Universitaire de Recherche Scientifique, Université de Pau, Avenue de l’Université, 64000 Pau, France
* Author to whom correspondence should be addressed.
Received: 1 March 2010; in revised form: 13 April 2010 / Accepted: 13 April 2010 / Published: 15 April 2010
Abstract: Aqueous biphasic systems (ABS) provide an alternative and efficient approach for the extraction, recovery and purification of biomolecules through their partitioning between two liquid aqueous phases. In this work, the ability of hydrophilic phosphonium-based ionic liquids (ILs) to form ABS with aqueous K3PO4 solutions was evaluated for the first time. Ternary phase diagrams, and respective tie-lines and tie-lines length, formed by distinct phosphonium-based ILs, water, and K3PO4 at 298 K, were measured and are reported. The studied phosphonium-based ILs have shown to be more effective in promoting ABS compared to the imidazolium-based counterparts with similar anions. Moreover, the extractive capability of such systems was assessed for distinct biomolecules (including amino acids, food colourants and alkaloids). Densities and viscosities of both aqueous phases, at the mass fraction compositions used for the biomolecules extraction, were also determined. The evaluated IL-based ABS have been shown to be prospective extraction media, particularly for hydrophobic biomolecules, with several advantages over conventional polymer-inorganic salt ABS.
Keywords: ionic liquids; aqueous two-phase systems; phase diagrams; partition coefficients
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Louros, C.L.S.; Cláudio, A.F.M.; Neves, C.M.S.S.; Freire, M.G.; Marrucho, I.M.; Pauly, J.; Coutinho, J.A.P. Extraction of Biomolecules Using Phosphonium-Based Ionic Liquids + K3PO4 Aqueous Biphasic Systems. Int. J. Mol. Sci. 2010, 11, 1777-1791.
Louros CLS, Cláudio AFM, Neves CMSS, Freire MG, Marrucho IM, Pauly J, Coutinho JAP. Extraction of Biomolecules Using Phosphonium-Based Ionic Liquids + K3PO4 Aqueous Biphasic Systems. International Journal of Molecular Sciences. 2010; 11(4):1777-1791.
Louros, Cláudia L. S.; Cláudio, Ana Filipa M.; Neves, Catarina M. S. S.; Freire, Mara G.; Marrucho, Isabel M.; Pauly, Jérôme; Coutinho, João A. P. 2010. "Extraction of Biomolecules Using Phosphonium-Based Ionic Liquids + K3PO4 Aqueous Biphasic Systems." Int. J. Mol. Sci. 11, no. 4: 1777-1791.