Next Article in Journal
Robust Uptake of Magnetic Nanoparticles (MNPs) by Central Nervous System (CNS) Microglia: Implications for Particle Uptake in Mixed Neural Cell Populations
Next Article in Special Issue
Chromatin Fiber Dynamics under Tension and Torsion
Previous Article in Journal
Orientational Packing of a Confined Discotic Mesogen in the Columnar Phase
Previous Article in Special Issue
Anchoring of a Single Molecular Rotor and Its Array on Metal Surfaces using Molecular Design and Self-Assembly
Article Menu

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2010, 11(3), 956-966; doi:10.3390/ijms11030956

Light Dose is a Limiting Factor to Maintain Cell Viability in Fluorescence Microscopy and Single Molecule Detection

1
Institut für Angewandte Forschung, Hochschule Aalen, Beethovenstr. 1, D-73430 Aalen, Germany
2
Institut für Lasertechnologien in der Medizin und Meßtechnik an der Universität Ulm, Helmholtzstr. 12, D-89081 Ulm, Germany
*
Author to whom correspondence should be addressed.
Received: 14 January 2010 / Revised: 20 February 2010 / Accepted: 21 February 2010 / Published: 8 March 2010
(This article belongs to the Special Issue Single Molecules)
View Full-Text   |   Download PDF [881 KB, uploaded 19 June 2014]   |  

Abstract

A test system for cell viability based on colony formation has been established and applied to high resolution fluorescence microscopy and single molecule detection. Living cells were irradiated either by epi-illumination or by total internal reflection (TIR) of a laser beam, and light doses where at least 90% of irradiated cells survived were determined. These light doses were in the range of a few J/cm2 up to about 200 J/cm2 depending on the wavelength of illumination as well as on the presence or absence of a fluorescent dye (e.g., the membrane marker laurdan). In general, cells were less sensitive to TIR than to epi-illumination. However, comparably high light doses needed for repetitive excitation of single molecules limit the application of super-resolution microscopy to living cells. View Full-Text
Keywords: cell viability; light dose; fluorescence microscopy; TIR; single molecules cell viability; light dose; fluorescence microscopy; TIR; single molecules
Figures

This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Wagner, M.; Weber, P.; Bruns, T.; Strauss, W.S.L.; Wittig, R.; Schneckenburger, H. Light Dose is a Limiting Factor to Maintain Cell Viability in Fluorescence Microscopy and Single Molecule Detection. Int. J. Mol. Sci. 2010, 11, 956-966.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top