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Int. J. Mol. Sci. 2010, 11(3), 956-966; doi:10.3390/ijms11030956
Article

Light Dose is a Limiting Factor to Maintain Cell Viability in Fluorescence Microscopy and Single Molecule Detection

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Received: 14 January 2010; in revised form: 20 February 2010 / Accepted: 21 February 2010 / Published: 8 March 2010
(This article belongs to the Special Issue Single Molecules)
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Abstract: A test system for cell viability based on colony formation has been established and applied to high resolution fluorescence microscopy and single molecule detection. Living cells were irradiated either by epi-illumination or by total internal reflection (TIR) of a laser beam, and light doses where at least 90% of irradiated cells survived were determined. These light doses were in the range of a few J/cm2 up to about 200 J/cm2 depending on the wavelength of illumination as well as on the presence or absence of a fluorescent dye (e.g., the membrane marker laurdan). In general, cells were less sensitive to TIR than to epi-illumination. However, comparably high light doses needed for repetitive excitation of single molecules limit the application of super-resolution microscopy to living cells.
Keywords: cell viability; light dose; fluorescence microscopy; TIR; single molecules cell viability; light dose; fluorescence microscopy; TIR; single molecules
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Wagner, M.; Weber, P.; Bruns, T.; Strauss, W.S.L.; Wittig, R.; Schneckenburger, H. Light Dose is a Limiting Factor to Maintain Cell Viability in Fluorescence Microscopy and Single Molecule Detection. Int. J. Mol. Sci. 2010, 11, 956-966.

AMA Style

Wagner M, Weber P, Bruns T, Strauss WSL, Wittig R, Schneckenburger H. Light Dose is a Limiting Factor to Maintain Cell Viability in Fluorescence Microscopy and Single Molecule Detection. International Journal of Molecular Sciences. 2010; 11(3):956-966.

Chicago/Turabian Style

Wagner, Michael; Weber, Petra; Bruns, Thomas; Strauss, Wolfgang S. L.; Wittig, Rainer; Schneckenburger, Herbert. 2010. "Light Dose is a Limiting Factor to Maintain Cell Viability in Fluorescence Microscopy and Single Molecule Detection." Int. J. Mol. Sci. 11, no. 3: 956-966.



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