- freely available
Int. J. Mol. Sci. 2010, 11(2), 691-703; doi:10.3390/ijms11020691
Abstract: Phenolic esters have attracted considerable interest due to the potential they offer for peroxidase catalysed cross-linking of cell wall polysaccharides. Particularly, feruloyl residues undergo radical coupling reactions that result in cross-linking (intra-/intermolecular) between polysaccharides, between polysaccharides and lignin and, between polysaccharides and proteins. This review addresses for the first time different studies in which it is established that cross-linking by dehydrodiferulates contributes to maize’s defences to pests and diseases. Dehydrodiferulate cross-links are involved in maize defence mechanisms against insects such as the European, Mediterranean, and tropical corn borers and, storage pest as the maize weevil. In addition, cross-links are also discussed to be involved in genetic resistance of maize to fungus diseases as Gibberella ear and stalk rot. Resistance against insects and fungus attending dehydrodiferulates could go hand in hand. Quantitative trait loci mapping for these cell wall components could be a useful tool for enhancing resistance to pest and diseases in future breeding programs.
In the Poaceae family (grasses and cereals) the primary cell wall is built of a skeleton of cellulosic microfibrils embedded in a matrix composed mainly of hemicelluloses (arabinoxylans, xyloglucan and, in some tissues, mixed-linked glucans) and smaller amounts of pectins and glycoproteins . In addition, grasses have unusually high concentrations of ferulic and p-coumaric acids ester-linked to cell wall polymers (Figure 1) . Depending on the tissue and its stage of development, cell walls in C4 grasses tend to have higher levels of hydroxycinnamic acids than C3 grasses; as C4, maize and sorghum cell walls can contain up to 4% ferulates (monomers plus dimers) and up to 3% p-coumarate [3,4]. p-Coumarate is mainly esterified to the γ–position of phenylpropanoid sidechains of S units in lignin [5–7]. Although very small quantities of p-coumarate are esterified to arabinoxylans in immature tissues, most p-coumarate accretion occurs in tandem with lignification [8,9], making p-coumarate accumulation a convenient indicator of lignin deposition. Ferulates are intracellularly esterified to the C5-hydroxyl of α-l-arabinose sidechains of xylans and deposited into primary and secondary walls of a variety of grass tissues [10–12]. During cell wall deposition and lignification, xylans are cross-linked by peroxidase-mediated coupling of ferulate monomers into a complex array of dimers and trimers and by extensive copolymerization of these ferulates into lignin (Figure 1) .
2. Cell Wall Cross-Linking by Dehydrodiferulates
The structural and functional roles of plant cell walls are controlled by the composition and organization of individual wall components. Cross-linking of cell wall components is expected to have a marked influence on numerous wall properties such as accessibility, extensibility, plasticity, digestibility, and adherence. Phenolic esters have attracted considerable interest due to the potential they provide for peroxidase catalysed cross-linking of cell wall polysaccharides [14,15]. Dimers of ferulic and p-coumaric acids can be generated enzymatically by peroxidase (5,5’-diferulic acid) or through photochemical reactions (truxillic and trucinic acids) . In particular, feruloyl residues undergo radical coupling reactions that result in cross-linking between polysaccharides (intra-/intermolecularly) (Figure 2) [4,17], between polysaccharides and lignin [18,19] and, between polysaccharides and proteins .
Formation of oxidatively coupled ferulate dimers was first described in 1971 . Since then, a diverse range of dehydrodiferulates have been detected in various plant materials and were characterized as 8-5′, 5-5′, 8-O-4′, 8-8′, and 4-O-5′ coupled dehydrodimers [22–30]. In addition, certain specific isomers of triferulic acid [31–35] and tetraferulic acid  have been isolated and structurally elucidated from primary cell walls in grass and cereal species, particularly from maize bran.
Cross-linking of wall polysaccharides is of considerable interest in food chemistry, food technology and nutritional sciences, but also in neighbouring disciplines like agricultural chemistry and plant physiology . Ferulate dimers have been considered responsible for phenomena such as terminating the expansion of cell wall growth , cell stiffening , cell wall adhesion influencing the cripness and softening fruits and vegetables [38–41], gelling properties of pectins and arabinoxylans [39,42,43], insolubility of cereal dietary fibers , and limited cell wall degradability by ruminants [3,13,44,45]. Cross-linking by dehydrodiferulates also contributes to plants’ defences to pest and diseases as revised in detail in the current review.
3. Role of Dehydrodiferulates Compounds in Maize Resistance to Pests
3.1. Corn Borer Resistance
Lepidopteran stalk boring larvae cause significant economic losses to maize (Zea mays L.) production throughout the world. One of the major corn borer pests is the European corn borer (ECB) [Ostrinia nubilalis (Hübner)] . This pest reduces maize yield not only damaging kernels by direct feeding, but also tunneling stalks which cause plant lodging at harvest. ECB also acts as a vector for several Fusarium stalk-rot fungi which lead to a further destruction of the stalk. Infestation begins at an early phenological stage of the maize plant. First generation larvae fed on the whorl leaf tissues of juvenile plants, while subsequent generations attack maize at its reproductive stage. The main importance is on later generations larvae that feed on the stalk.
In studies of borer resistance, the content of isomers of dehydrodiferulic acids (DFAs) in corn leaves was highly and negatively correlated across genotypes of maize with field leaf damage caused by ECB . In a later study, those authors suggest that early cell wall fortification through phenolic cross-linking of hemicellulose by diferulic acid increases leaf tissue toughness, and therefore, decreases neonate larvae penetration into leaf tissues . Additional confirmation was provided by the observation that DFA content of maize increased in different tissues over cycles of selection for borer resistance in the Iowa stiff stalk synthetic BS9 . High fibre content alone may increase the bulk density of the tissue, but in presence of phenolic-carbohydrate complexes, fibre strength may increase, providing a tougher physical barrier to restrict insect penetration and render nutrients within the tissues less accessible . Besides, in the same study, both DFA and p-coumaric acids were negatively related with damage parameters for rind, node and pith tissue .
Ramputh  extended previous research with the observation that resistance to important tropical and subtropical stem borers could be sensitive to early cell wall fortification by phenolic cross-linking. Host plant resistance in maize to the southwestern corn borer (Diatraea grandiosella Dyar) and the sugarcane borer [Diatraea saccharalis (Fabricius)] was investigated in relation to bound phenolics in the leaf cell wall. In that study, dehydrodiferulic acids were found to be significantly correlated to tissue toughness and significant negative correlations were found between DFA and leaf feeding damage by both insects.
The oxidative potential of H2O2 to contribute to cell wall strengthening could retard insect feeding through the oxidative cross linking of specific cell wall structural proteins , polymerization of coniferyl alcohol affecting lignin formation  and cell wall peroxidase mediated cross linking of hemicellulose microfibrils with diferulic acid DFA . A molecular transformation of maize was employed to increase cell wall hydrogen peroxide in order to enhance DFA formation and European corn borer resistance . Corn transformed with a wheat oxalate oxidase gene (germin) under the control of the rice promoter elements (pActOXO) expressed the enzyme activity constitutively. Field testing showed that the transgenic maize exhibited more resistance to ECB than the non-transgenic counterpart. However, the transformation did not increase DFA formation as predicted, and resistance was associated with the direct effects of H2O2 on insect physiology. In a subsequent study was found that transgenic oxalate oxidase elicits defence responses by generation of H2O2, increasing soluble phenolics contents, and activating jasmonic acid signaling .
Recent studies evaluated the putative role of dehydrodiferulate contents in maize pith and leaf-sheaths in the resistance against the Mediterranean corn borer (MCB) (Sesamia nonagrioides Lef.) [56–58]. Three hydroxycinnamates, p-coumaric, trans-ferulic, and cis-ferulic acids, and three dehydrodiferulates, 8-5′, 8-O-4′, and 8-5′ b (benzofuran form), were identified . The amount of all these compounds in the pith was correlated with the resistance level in the genotypes, with the resistant inbreds having the highest concentrations . In addition, significant negative correlations were found between larvae weight reared on leaf-sheaths and diferulic acid content for six genotypes of the seven evaluated .
The genotypes used in those studies were a limited number of inbred lines with diverse background and perhaps exhibiting different mechanisms of defence. Therefore, the real relationship between DFA content and resistance was not established because it could be biased by background differences among genotypes. In order to remove this bias, several cycles of selection for MCB resistance derived from the synthetic population EPS12 were evaluated for cell-wall phenolic concentrations in the pith tissues . In this sense, higher concentrations of total DFAs were associated with less tunnel length and number of larvae per stem . Santiago and co-workers  showed new and concrete evidence that the cell-wall bound phenolics could have a significant role in the resistance to the MCB, although current development of divergent recurrent selection cycles maximizing differences for DFA concentrations will definitively clarify the role of DFAs in the resistance to the Mediterranean corn borer larvae. In 2007, the divergent selection for total DFAs content in the pith was initiated from the F2 derived from the cross of two inbred lines, CO125 (high DFA content) and PB130 (low DFA content).
3.2. Maize Weevil Resistance
The maize weevil (MW) (Sitophilus zeamais Motsch.) is a destructive insect feeding on stored maize throughout the world . Subsistence farmers in tropical and subtropical agroecosystems often experience grain damage exceeding 30% during on-farm storage . Various biochemical and physical characteristics have been identified as mechanisms of kernel resistance to MW [61–65]. Phenolic acids have been studied extensively as biochemical components correlated with resistance and found to act in two ways: through mechanical resistance (cell wall bound hydroxycinnamic acids) and antibiosis (phenolic acid amides) in the pericarp and aleurone layer, respectively . Arnason and coworkers  has demonstrated that MW resistant genotypes have higher concentrations of total DFAs in the whole kernel. These dimers probably contribute to the observed correlations between phenolic acid content and grain hardness [65,66].
To understand the importance of dehydrodiferulates in MW resistance, a detailed analysis of DFAs in the pericarp was conducted by García-Lara and co-workers . The analysis revealed the presence of three major simple phenolic acids (p-coumaric acid, cis-ferulic acid and trans-ferulic acid) and four isomers of DFAs (8-5′ DFA, 5-5′ DFA, 8-O-4′ DFA, 8-5′b DFA). In that study, DFA accounted for 8% of the total phenolic acid content, with resistant genotypes having a three-fold higher level than susceptible genotypes. On the basis of step-wise regression models, 5-5′ DFA, 8-O-4′ DFA, p-coumaric acid, and trans-ferulic acid were the most important phenolic acids in explaining phenotypic variance for MW resistance. Besides, weevil susceptibility was negatively correlated with total DFAs. In conclusion, maize genotypes with elevated levels of cell wall cross-linking components in the pericarp were more resistant to MW .
4. Role of Dehydrodiferulates Compounds in Maize Resistance to Fungal Diseases
4.1. Gibberella Ear Rot Resistance
Fusarium graminearum Schwabe, [teleomorph Gibberella zeae (Schwein.) Petch], is responsible for one of the major fungal diseases occurring sporadically in cool and temperate maize cultivation areas . This disease known as Gibberella ear rot, is a cause of great concern because of the ability of this mold to produce mycotoxins, such as trichothecenes and zearalenone that affect grain quality and also represent a potential risk for livestock and human health [69–71].
Previous studies on maize indicate that phenolic compounds such as inducible flavones in silks  and structural phenolics in kernels [73,74] may be involved in maize resistance to ear rot. Attending to these results, Bily and co-workers  determined the concentrations of dehydrodimers of ferulic acid in the pericarp and aleurone tissues of some maize genotypes of varying susceptibility. Significant negative correlations were found between disease severity and total DFAs content, and even stronger negative correlations were observed between total DFA and ergosterol, a fungal component of the cell membrane which is an indicator of fungal biomass in infected plant tissues . DFAs are known to stabilize the wall at the early stage of plant development . It is also known that DFAs impede in vitro cell-wall degradation by fungal hydrolases, such as Aspergillus esterases . In Bily’s study , initial penetration in the ears was mechanically facilitated, but mycelial progression on intact neighboring grains was reduced in maize genotypes with high levels of DFAs. Besides, F. graminearum, as many other pathogenic fungi, has a large spectrum of extracellular enzymatic activities [78,79], and the activity of fungal esterases can release free forms of ferulic ester from maize tissues. Once released, free ferulate may inhibit the ability of Fusarium to produce mycotoxins .
4.2. Gibberella Stalk Rot Resistance
Fusarium graminearum is only one of many species responsible for stalk rot, but is considered to possess the highest pathogenicity (the ability to cause disease) and aggressiveness (the amount of disease caused) [81,82]. These fungal organisms decay pith tissue in the lower stalk internodes, which can often result in premature plant death, poor kernel filling and/or lodging. Besides, trichothecenes such as deoxynivalenol (DON) is water soluble, and translocation in the phloem as a bulk flow of solution is assumed to occur [81,83]. DON produced in the stalk or ear of maize can be found in tissues not invaded by F. graminearum [84,85].
Phenolic compounds have been shown to inhibit in vitro growth of several fungal genera [86,87]; significant inhibitory effects were reported for Fusarium species [73,88,89]. In addition, evidence strongly suggests that esterification of phenols to cell-wall materials is a common theme in the expression of resistance [73–75,90]. Therefore, Santiago and co-workers  tried to determine if a relationship exists between phenolic compounds and resistance to stalk infection by F. graminearum monitoring field-grown inbred lines of maize after artificial inoculation. Reinforcing the possible role of DFAs in fungal resistance, significant negative correlations between DFAs contents and disease severity ratings four days after inoculation were found. The abundance of DFAs in cell walls makes polysaccharides less sensitive to the cell wall-degrading enzymes of pathogens [92,93]. In this case, the DFAs could function in the pith tissues as preformed resistance barriers prior to infection. Low contents of DFAs at early stages of infection would leave time for the pathogen to develop abundantly and to produce toxins, breaking a possible resistance response and leading to typical symptoms of disease.
In summary, cross-linking of plant cell wall polymers by dehydrodiferulates contributes consistently to the maize’s defence mechanisms against pests and diseases. Dehydrodiferulate cross-links are involved in the defence mechanisms against insects such as the European and Mediterranean corn borer, the southwestern corn borer, the sugarcane borer, and the maize weevil. In addition, DFA cross-links are also discussed to be involved in genetic resistance of maize to Gibberella ear rot and stalk rot. These compounds have been related with resistance in a wide range of maize tissues as leaf, kernel, rind, or pith. The role of dehydrodiferulates in the cell wall fortification has been noted in the literature, and this structural resistance could be more difficult to overcome than an antibiotic defence. Resistance against insects and fungi due to dehydrodiferulates content could go hand in hand. Quantitative trait loci mapping for these cell wall components could be a useful tool for enhancing resistance to pest and diseases in the future.
|ECB||european corn borer|
|MCB||mediterranean corn borer|
The authors acknowledge all workers and collaborators who participated in pest and diseases research studies. Most of the latest corn borer studies by the authors’ group were supported by the Plan Nacional I+D+I (AGL2003-00961, AGL2006-1314, AGL2009-09611). R. Santiago acknowledges a “Juan de la Cierva” postdoctoral contract partially financed by the European Social Fund.
References and Notes
- Carpita, NC; Defernez, M; Findlay, K; Wells, B; Shoue, DA; Catchpole, G; Wilson, RH; McCann, MC. Cell wall architecture of the elongating maize coleoptile. Plant Physiol 2001, 127, 551–565. [Google Scholar]
- Ishii, T. Structure and functions of feruloylated polysaccharides. Plant Sci 1997, 127, 111–127. [Google Scholar]
- Hatfield, RD; Wilson, JR; Mertens, DR. Composition of cell walls isolated from cell types of grain sorghum stems. J. Sci. Food Agric 1999, 79, 891–899. [Google Scholar]
- Saulnier, L; Thibault, JF. Ferulic acid and diferulic acids as components of sugar-beet pectins and maize bran heteroxylans. J. Sci. Food Agric 1999, 79, 396–402. [Google Scholar]
- Ralph, J; Hatfield, RD; Quideau, S; Helm, RF; Grabber, JH; Jung, HJG. Pathway of p-coumaric acid incorporation into maize lignin as revealed by NMR. J. Am. Chem. Soc 1994, 116, 9448–9456. [Google Scholar]
- Grabber, JH; Quideau, S; Ralph, J. p-Coumaroylated syringyl units in maize lignin; implications for β-ether cleavage by thioacidolysis. Phytochemistry 1996, 43, 1189–1194. [Google Scholar]
- Lu, F; Ralph, J. Detection and determination of p-coumaroylated units in lignins. J. Agric. Food Chem 1999, 47, 1988–1992. [Google Scholar]
- Morrison, TA; Jung, HJG; Buxton, DR; Hatfield, RD. Cell-wall composition of maize internodes of varying maturity. Crop Sci 1998, 38, 455–460. [Google Scholar]
- Vailhe, MAB; Provan, GJ; Scobbie, L; Chesson, A; Maillot, MP; Cornu, A; Besle, JM. Effect of phenolic structures on the degradability of cell walls isolated from newly extended apical internode of tall fescue (Festuca arundinacea Schreb.). J. Agric. Food Chem 2000, 48, 618–623. [Google Scholar]
- Migne, C; Prensier, G; Utille, JP; Angibeaud, P; Cornu, A; Grenet, E. Immunocytochemical localisation of p-coumaric acid and feruloyl-arabinose in the cell walls of maize stem. J. Sci. Food Agric 1998, 78, 373–381. [Google Scholar]
- MacAdam, JW; Grabber, JH. Relationship of growth cessation with the formation of diferulate cross-links and p-coumaroylated lignins in tall fescue. Planta 2002, 215, 785–793. [Google Scholar]
- Jung, HJG. Maize stem tissues: Ferulate deposition in developing internode cell walls. Phytochemistry 2003, 63, 543–549. [Google Scholar]
- Grabber, JH; Ralph, J; Lapierre, C; Barrière, Y. Genetic and molecular basis of grass cell wall degradability. I. Lignin-cell wall matrix interactions. Comptes Rendus Biol 2004, 327, 455–465. [Google Scholar]
- Biggs, KJ; Fry, SC. Phenolic cross-linking in the plant cell wall. In Physiology of Expansion During Plant Growth: Proceedings of the Second Annual Pennsylvania State Symposium in Plant Physiology; Cosgrove, DJ, Knieval, DP, Eds.; American Society of Plant Physiologists: Rockville, PA, USA, 1987; pp. 46–57. [Google Scholar]
- Brett, CT; Waldron, KW. Cell walls in diet and health. In Physiology and Biochemistry of Plant Cell Walls; Black, M, Charlwood, BV, Eds.; Chapman and Hall: London, UK, 1996; pp. 222–238. [Google Scholar]
- Bunzel, M. Chemistry and occurrence of hydroxycinnamate oligomers. Phytochem Rev 2009. doi 10.1007/s11101-009-9139-3.. [Google Scholar]
- Allerdings, E; Ralph, J; Schatz, PF; Gniechwitz, D; Steinhart, H; Bunzel, M. Isolation and structural identification of diarabinosyl 8-O-4-dehydrodiferulate from maize bran insoluble fibre. Phytochemistry 2005, 66, 113–124. [Google Scholar]
- Ralph, J; Grabber, JH; Hatfield, RD. Lignin-ferulate crosslinks in grasses: Active incorporation of ferulate polysaccharide esters into ryegrass lignins. Carbohydr. Res 1995, 275, 167–178. [Google Scholar]
- Grabber, JH; Ralph, J; Hatfield, RD. Cross-linking of maize walls by ferulate dimerization and incorporation into lignin. J. Agric. Food Chem 2000, 48, 6106–6113. [Google Scholar]
- Piber, M; Koehler, P. Identification of dehydro-ferulic acidtyrosine in rye and wheat: Evidence for a covalent cross-link between arabinoxylans and proteins. J. Agric. Food Chem 2005, 53, 5276–5284. [Google Scholar]
- Geissmann, T; Neukom, H. Vernetzung von Phenolcarbonsäureestern von Polysacchariden durch oxydative phenolische Kupplung. Helv. Chim. Acta 1971, 54, 1108–1112. [Google Scholar]
- Markwalder, HU; Neukom, H. Diferulic acid as a possible cross-link in hemicelluloses from wheat germ. Phytochemistry 1976, 15, 836–837. [Google Scholar]
- Nordkvist, E; Salomonsson, AC; Aman, P. Distribution of insoluble bound phenolic acids in barley grain. J. Sci. Food Agric 1984, 35, 657–661. [Google Scholar]
- Kamisaka, S; Takeda, S; Takahashi, K; Shibata, K. Diferulic and ferulic acids in the cell wall of oat coleoptiles—their relationships to mechanical properties of the cell wall. Physiol. Plant 1990, 78, 1–7. [Google Scholar]
- Ralph, J; Quideau, S; Grabber, JH; Hatfield, RD. Identification and synthesis of new ferulic acid dehydrodimers present in grass cell walls. J. Chem. Soc. Perkin Trans 1994, 1, 3485–3498. [Google Scholar]
- Parr, AJ; Waldron, KW; Ng, A; Parker, ML. The wall-bound phenolics of Chinese water chestnut (Eleocharis dulcis). J. Sci. Food Agric 1996, 71, 501–507. [Google Scholar]
- Parr, AJ; Ng, A; Waldron, KW. Ester-linked phenolic components of carrot cell walls. J. Agric. Food Chem 1997, 45, 2468–2471. [Google Scholar]
- Saulnier, L; Thibault, JF. Ferulic acid and diferulic acids as components of sugar-beet pectins and maize bran heteroxylans. J. Sci. Food Agric 1999, 79, 396–402. [Google Scholar]
- Bunzel, M; Ralph, J; Marita, J; Steinhart, H. Identification of 4-O-5-coupled diferulic acid from insoluble cereal fiber. J. Agric. Food Chem 2000, 48, 3166–3169. [Google Scholar]
- Bunzel, M; Ralph, J; Marita, JM; Hatfield, RD; Steinhart, H. Diferulates as structural components in soluble and insoluble cereal dietary fibre. J. Sci. Food Agric 2001, 81, 653–660. [Google Scholar]
- Bunzel, M; Funk, C; Steinhart, H. Semipreparative isolation of dehydrodiferulic and dehydrotriferulic acids as standard substances from maize bran. J. Sep. Sci 2004, 27, 1080–1086. [Google Scholar]
- Bunzel, M; Ralph, J; Funk, C; Steinhart, H. Structural elucidation of new ferulic acid-containing phenolic dimers and trimers isolated from maize bran. Tetrahedron Lett 2005, 46, 5845–5850. [Google Scholar]
- Bunzel, M; Ralph, J; Brüning, P; Steinhart, H. Structural identification of dehydrotriferulic and dehydrotetraferulic acids from insoluble maize bran fiber. J. Agric. Food Chem 2006, 54, 6409–6418. [Google Scholar]
- Rouau, X; Cheynier, V; Surget, A; Gloux, D; Barron, C; Meudec, E; Louis-Montero, J; Criton, M. A dehydrotrimer of ferulic acid from maize bran. Phytochemistry 2003, 63, 899–903. [Google Scholar]
- Funk, C; Ralph, J; Steinhart, H; Bunzel, M. Isolation and structural characterisation of 8–O–4/8–O–4- and 8–8/8–O–4-coupled dehydrotriferulic acids from maize bran. Phytochemistry 2005, 66, 363–371. [Google Scholar]
- Ralph, J; Bunzel, M; Marita, JM; Hatfield, RD; Lu, F; Kim, H; Schatz, PF; Grabber, JH; Steinhart, H. Peroxidase-dependent cross-linking reactions of p-hydroxycinnamates in plant cell walls. Phytochem. Rev 2004, 3, 79–96. [Google Scholar]
- Zarra, I; Sanchez, M; Queijeiro, E; Pena, MJ; Revilla, G. The cell wall stiffening mechanism in Pinus pinaster Aiton: Regulation by apoplastic levels of ascorbate and hydrogen peroxide. J. Sci. Food Agric 1999, 79, 416–420. [Google Scholar]
- Waldron, KW; Smith, AC; Parr, AJ; Ng, A; Parker, ML. New approaches to understanding and controlling cell separation in relation to fruit and vegetable texture. Trends Food Sci. Technol 1997, 8, 213–221. [Google Scholar]
- Ng, A; Harvey, AJ; Parker, ML; Smith, AC; Waldron, KW. Effect of oxidative coupling on the thermal stability of texture and cell wall chemistry of beet boot (Beta vulgaris). J. Agric. Food Chem 1998, 46, 3365–3370. [Google Scholar]
- Parker, CC; Parker, ML; Smith, AC; Waldron, KW. Thermal stability of texture in Chinese water chestnut may be dependent on 8, 80-diferulic acid (aryltetralyn form). J. Agric. Food Chem 2003, 51, 2034–2039. [Google Scholar]
- Waldron, KW; Parker, ML; Smith, AC. Plant cell walls and food quality. Comp. Rev. Food Sci. Food Safety 2003, 2, 128–146. [Google Scholar]
- Robertson, JA; Faulds, CB; Smith, AC; Waldron, KW. Peroxidase-mediated oxidative cross-linking and its potential to modify mechanical properties in water-soluble polysaccharide extracts and cereal grain residues. J. Agric. Food Chem 2008, 56, 1720–1726. [Google Scholar]
- Oosterveld, A; Beldman, G; Voragen, AGJ. Oxidative cross-linking of pectic polysaccharides from sugar beet pulp. Carbohydr. Res 2000, 328, 199–207. [Google Scholar]
- Grabber, JH. How do lignin composition, structure, and cross-linking affect degradability? A review of cell wall model studies. Crop Sci 2005, 45, 820–831. [Google Scholar]
- Jung, HJG; Casler, MD. Maize stem tissues: Impact of development on cell wall degradability. Crop Sci 2006, 46, 1801–1809. [Google Scholar]
- Gianessi, L; Sankula, S; Reigner, N. Plant Biotechnology: Potential impact for improving pest management in european agriculture, maize case study. The National Center for Food and Agricultural Policy, 2003. Full report available: http://www.ncfap.org/documents/ExecutiveSummaryJune.pdf (accessed November 12, 2009).. [Google Scholar]
- Bergvinson, DJ; Arnason, JT; Hamilton, RI; Mihm, JA; Jewell, DC. Determining leaf toughness and its role in maize resistance to the European corn borer (Lepidoptera: Pyralidae). J. Econ. Entomol 1994, 87, 1743–1748. [Google Scholar]
- Bergvinson, DJ; Hamilton, RI; Arnason, JT. Leaf profile of maize resistance factors to European corn borer, Ostrinia nubilalis. J. Chem. Ecol 1995, 21, 343–354. [Google Scholar]
- Bergvinson, DJ; Arnason, JT; Hamilton, RI. Phytochemical changes during recurrent selection for resistance to the European corn borer. Crop Sci 1997, 37, 1567–1572. [Google Scholar]
- Ramputh, AI. Soluble and cell wall Bound phenolic-mediated insect resistance in corn and sorghum; Ottawa-Carleton Institute of Biology: Ottawa, ON, Canada, 2002. [Google Scholar]
- Brisson, LF; Tenhaken, R; Lamb, C. Function of oxidative cross-linking of cell wall structural proteins in plant disease resistance. Plant Cell 1994, 6, 1703–1712. [Google Scholar]
- Richardson, A; Stewart, D; McDougall, GJ. Identification and partial characterization of a coniferyl alcohol oxidase from lignifying xylem of Sitka spruce (Picea sitchensis). Planta 1997, 203, 35–43. [Google Scholar]
- Fry, SC. Cross-linking of matrix polymers in the growing cell walls of angiosperms. Ann. Rev. Plant Physiol 1986, 37, 165–186. [Google Scholar]
- Ramputh, AI; Arnason, JT; Cass, L; Simmonds, JA. Reduced herbivory of the European corn borer (Ostrinia nubilalis) on corn transformed with germin, a wheat oxalate oxidase gene. Plant Sci 2002, 162, 431–440. [Google Scholar]
- Mao, J; Burt, AJ; Ramputh, A-I; Simmonds, J; Cass, L; Hubbard, K; Miller, S; Altosaar, I; Arnason, JT. Diverted secondary metabolism and improved resistance to European corn borer (Ostrinia nubilalis) in maize (Zea mays L.) transformed with wheat oxalate oxidase. J. Agric. Food Chem 2007, 55, 2582–2589. [Google Scholar]
- Santiago, R; Butrón, A; Arnason, JT; Reid, LM; Souto, XC; Malvar, RA. Putative role of pith cell wall phenylpropanoids in Sesamia nonagrioides (Lepidoptera: Noctuidae) resistance. J. Agric. Food Chem 2006, 54, 2274–2279. [Google Scholar]
- Santiago, R; Butrón, A; Reid, LM; Arnason, JT; Sandoya, G; Souto, XC; Malvar, RA. Diferulate content of maize sheaths is associated with resistance to the Mediterranean corn borer Sesamia nonagrioides (Lepidoptera: Noctuidae). J. Agric. Food Chem 2006, 54, 9140–9144. [Google Scholar]
- Santiago, R; Sandoya, G; Butrón, A; Barros, J; Malvar, RA. Changes in phenolic concentrations during recurrent selection for resistance to the Mediterranean corn borer (Sesamia nonagrioides Lef.). J. Agric. Food Chem 2008, 56, 8017–8022. [Google Scholar]
- Markham, RH; Bosque-Pérez, NA; Borgemeister, C; Meikle, WG. Developing pest management strategies for the maize weevil, Sitophilus zeamais, and the large grain borer, Prostephanus truncatus, in the humid and sub-humid tropics. FAO Plant Prot. Bull 1994, 42, 125–136. [Google Scholar]
- Tigar, BJ; Osborne, PE; Key, GE; Flores-S, ME; Vazquez, AM. Insect pest associated with rural maize stores in Mexico with particular reference to Prostephanus truncatus (Coleoptera: Bostrichidae). J. Stored Prod. Res 1994, 30, 267–281. [Google Scholar]
- Dobie, P. The contribution of the Tropical Stored Products Centre to the study of insect resistance in stored maize. Trop. Stored Prod. Inf 1977, 34, 7–22. [Google Scholar]
- Serratos, A; Arnason, JT; Nozzolillo, C; Lambert, JDH; Philogène, BJR; Fulcher, G; Davidson, K; Peacock, L; Atkinson, J; Morand, P. Factors contributing to resistance of exotic maize populations to maize weevil, Sitophilus zeamais. J. Chem. Ecol 1987, 13, 751–762. [Google Scholar]
- Tipping, PW; Legg, DE; Rodriguez, JG; Poneleit, CG. Influence of maize pericarp surface relief on the resistance to the maize weevil. J. Kans. Entomol. Soc 1988, 61, 237–241. [Google Scholar]
- Arnason, JT; Baum, B; Gale, J; Lambert, JDH; Bergvinson, D; Philogène, BJR; Serratos, JA; Mihm, JA; Jewell, DC. Variation in resistance of Mexican landraces of maize to maize weevil Sitophilus zeamais, in relation to taxonomic and biochemical parameters. Euphytica 1994, 74, 227–236. [Google Scholar]
- Arnason, JT; Conilh de Beyssac, B; Philogène, BJR; Bergvinson, D; Serratos, JA; Mihm, JA. Mechanism of resistance in maize grain to the maize weevil and the larger grain borer. In Insect Resistance Maize: Recent Advances and Utilization; Proceedings of an International Symposium; Mexico, DF, Ed.; Mexico, 1997; pp. 91–95. [Google Scholar]
- Classen, D; Arnason, JT; Serratos, JA; Lambert, JDH; Nozzolillo, C; Philogène, BJR. Correlation of phenolic acid content of maize to resistance to Sitophilus zeamais, the maize weevil, in CIMMYT’s collections. J. Chem. Ecol 1990, 16, 301–315. [Google Scholar]
- García-Lara, S; Bergvinson, D; Burt, AJ; Ramputh, A-I; Díaz-Pontones, DM; Arnason, JT. The role of pericarp cell wall components in maize weevil resistance. Crop Sci 2004, 44, 1546–1552. [Google Scholar]
- Sutton, JC. Epidemiology of wheat head blight and maize ear rot caused by Fusarium graminearum. Can. J. Plant Pathol 1982, 4, 195–209. [Google Scholar]
- Mirocha, CJ; Pathre, SY; Schauerhamer, B; Christensen, CM. Natural occurrence of Fusarium toxins in feedstuff. Appl. Environ. Microbiol 1976, 32, 553–556. [Google Scholar]
- Pitt, JI. Toxigenic fungi and mycotoxins. Br. Med. Bull 2000, 56, 184–192. [Google Scholar]
- Steyn, PS; Stander, MA. Mycotoxins as causal factors of diseases in humans. J. Toxicol. Toxin Rev 1999, 18, 229–243. [Google Scholar]
- Reid, LM; Mather, DE; Arnason, JT; Hamilton, RI; Bolton, AT. Changes in phenolic constituents of maize silk infected with Fusarium graminearum. Can. J. Bot 1992, 70, 1697–1702. [Google Scholar]
- Assabgui, RA; Reid, LM; Hamilton, RI; Arnason, JT. Correlation of kernel (E)-ferulic acid content of maize with resistance to Fusarium graminearum. Phytopathology 1993, 83, 949–953. [Google Scholar]
- Miller, JD; Miles, M; Davis, AF. Kernel concentrations of 4-acetylbenzoxazolin-2-one and diferuloylputrescine in maize genotypes and Gibberella ear rot. J. Agric. Food Chem 1997, 45, 4456–4459. [Google Scholar]
- Bily, AC; Reid, LM; Taylor, JH; Johnston, D; Malouin, C; Buró, AJ; Bakan, B; Regnault-Roger, C; Pauls, KP; Arnason, JT; Philogène, BJR. Dehydrodimers of ferulic acid in maize grain pericarp and aleurone: Resistance factors to Fusarium graminearum. Phytopathology 2003, 93, 712–719. [Google Scholar]
- Grabber, JH; Hatfield, RH; Ralph, J. Diferulate cross-links impede the enzymatic degradation of non-lignified maize walls. J. Sci. Food Agric 1998, 77, 193–200. [Google Scholar]
- Obel, N; Porchia, AC; Scheller, HV. Dynamic changes in cell wall polysaccharides during wheat seedling development. Phytochemistry 2002, 60, 603–610. [Google Scholar]
- Manka, M. Cellulolytic and pectolytic activity of Fusarium isolates pathogenic to corn seedlings. Acta Microbiol. Pol 1980, 30, 25–32. [Google Scholar]
- Huang, Z; White, DG; Payne, GA. Corn seed proteins inhibitory to Aspergillus flavus and aflatoxin biosynthesis. Phytopathology 1997, 87, 622–627. [Google Scholar]
- Bakan, B; Bily, AC; Melcion, D; Cahagnier, B; Regnault-Roger, C; Philogène, BJR; Richard-Molard, D. Possible role of plant phenolics in the production of trichothecenes by Fusarium graminearum strains on different fractions of maize kernels. J. Agric. Food Chem 2003, 51, 2826–2831. [Google Scholar]
- Snijders, CH. A breeding for resistance to Fusarium in wheat and maize. In Mycotoxins in Grain: Compounds Other than Aflatoxin; Miller, JD, Trenholm, HL, Eds.; Eagan Press: St. Paul, MN, USA, 1994. [Google Scholar]
- Gilberton, RL; Brown, WM; Ruppel, EG. Prevalence and virulence of Fusarium spp. associated with stalk rot of corn in Colorado. Plant Dis 1985, 69, 1065–1068. [Google Scholar]
- Marasas, WFO; Nelson, PE; Tousson, TA. Toxigenic Fusarium Species: Identity and Mycotoxicology; The Pennsylvania State University Press: Pennsylvania, USA, 1984. [Google Scholar]
- Miller, JD; Young, JC; Trenholm, HL. Fusarium toxins in field corn. I. Time course of fungal growth and production of deoxynivalenol and other mycotoxins. Can. J. Bot 1983, 61, 3080–3087. [Google Scholar]
- Young, JC; Miller, JD. Appearance of fungus, ergosterol and Fusarium mycotoxins in the husk, axial stem and stalk after ear inoculation of field corn. Can. J. Plant Sci 1985, 65, 47–53. [Google Scholar]
- Purushothaman, D. Changes in phenolic compounds in rice cultivars as influenced by Xanthomonas oryzae. Il Riso 1976, 25, 88–91. [Google Scholar]
- Baranowski, JD; Davidson, PM; Nagel, CW; Branen, AL. Inhibition of Saccharomyces cerevisiae by naturally occurring hydroxycinnamates. J. Food Sci 1980, 45, 592–594. [Google Scholar]
- Kasenberg, TR; Traquair, JA. Effects of phenolics on growth of Fusarium oxysporum f. spradicis-lycopersici in vitro. Can. J. Bot 1988, 66, 1174–1177. [Google Scholar]
- McKeehen, JD; Busch, RH; Fulcher, RG. Evaluation of wheat (Triticum aestivum L.) phenolic acids during grain development and their contribution to Fusarium resistance. J. Agric. Food Chem 1999, 47, 1476–1482. [Google Scholar]
- Fry, SC. Intracellular feruloylation of pectic polysaccharides. Planta 1987, 171, 205–211. [Google Scholar]
- Santiago, R; Reid, LM; Arnason, JT; Zhu, XY; Martinez, N; Malvar, RA. Phenolics in maize genotypes differing in susceptibility to Gibberella stalk rot (Fusarium graminearum Schwabe). J. Agric. Food Chem 2007, 55, 5186–5193. [Google Scholar]
- Ikegawa, T; Mayama, S; Nakayashiki, H; Kato, H. Accumulation of diferulic acid during the hypersensitive response of oat leaves to Puccinia coronata f. sp. avena and its role in the resistance of oat tissues to cell wall degrading enzymes. Physiol. Mol. Plant Pathol 1996, 48, 245–256. [Google Scholar]
- Matern, U; Grimmig, B. Polyphenols in plant pathology. In Polyphenolic Phenomena; Scalbert, A, Ed.; INRA Editions: Paris, France, 1993. [Google Scholar]
© 2010 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).