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Int. J. Mol. Sci. 2010, 11(2), 647-655; doi:10.3390/ijms11020647

Isolation of Sulfur Reducing and Oxidizing Bacteria Found in Contaminated Drywall

RealTime Laboratories, LLC, 13016 Bee Street #203, Dallas, TX 79234, USA
PRO-LAB, Weston, FL 33326, USA
Department of Microbiology and Immunology, Texas Tech University Health Sciences Center, Lubbock, TX 79430, USA
Neuro-Test, Inc., Pasadena, CA 91107, USA
Embark Scientific, LLC, Austin, TX 78757, USA
Author to whom correspondence should be addressed.
Received: 22 January 2010 / Accepted: 29 January 2010 / Published: 5 February 2010
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
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Drywall from China has been reported to release sulfur producing products which are corrosive to metals, result in noxious odors, and represent a significant health risk. It has been reported that these emissions produce medical symptoms such as respiratory or asthma type problems, sinusitis, gastrointestinal disorders, and vision problems in home owners and their household pets. We report here a method of identifying a causative agent for these emissions by sampling affected gypsum wallboard and subjecting those samples to Real Time Polymerase Chain Reaction [RT-PCR] studies. Specific DNA probes and primers have been designed and patented that detect a specific iron and sulfur reducing bacterium (i.e., Thiobacillus ferrooxidans). One hundred percent of affected drywall samples obtained from homes located in the southeastern United States tested positive for the presence of T. ferrooxidans. All negative controls consisting of unaffected wallboard and internal controls, Geotrichum sp., tested negative within our limits of detection. View Full-Text
Keywords: Chinese drywall; Thiobacillus ferrooxidans; sulfur reducing bacteria; sulfur emissions Chinese drywall; Thiobacillus ferrooxidans; sulfur reducing bacteria; sulfur emissions

This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Hooper, D.G.; Shane, J.; Straus, D.C.; Kilburn, K.H.; Bolton, V.; Sutton, J.S.; Guilford, F.T. Isolation of Sulfur Reducing and Oxidizing Bacteria Found in Contaminated Drywall. Int. J. Mol. Sci. 2010, 11, 647-655.

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