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Int. J. Mol. Sci. 2010, 11(12), 5056-5064; doi:10.3390/ijms11125056

A Simple and Rapid Method for DNA Isolation from Xylophagous Insects

1
Center for Ecosystems Research (CIEco), National Autonomous University of Mexico (UNAM), Morelia, Mexico
2
Multidisciplinary Center of Biotechnology Studies (CMEB), Michoacan University of San Nicolas of Hidalgo (UMSNH), Morelia, Mexico
*
Author to whom correspondence should be addressed.
Received: 8 October 2010 / Revised: 22 November 2010 / Accepted: 30 November 2010 / Published: 7 December 2010
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract

Published methods to isolate DNA from insects are not always effective in xylophagous insects because they have high concentrations of phenolics and other secondary plant compounds in their digestive tracts. A simple, reliable and labor-effective cetyltrimethylammonium bromide-polyvinylpyrrolidone (CTAB-PVP) method for isolation of high quality DNA from xylophagous insects is described. This method was successfully applied to PCR and restriction analysis, indicating removal of common inhibitors. DNA isolated by the CTAB-PVP method could be used in most molecular analyses. View Full-Text
Keywords: Cerambycidae; DNA isolation; PCR; Oncideres albomarginata chamela; phenolics; restriction digestion; xylophagous insects Cerambycidae; DNA isolation; PCR; Oncideres albomarginata chamela; phenolics; restriction digestion; xylophagous insects
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Calderón-Cortés, N.; Quesada, M.; Cano-Camacho, H.; Zavala-Páramo, G. A Simple and Rapid Method for DNA Isolation from Xylophagous Insects. Int. J. Mol. Sci. 2010, 11, 5056-5064.

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