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A Simple and Rapid Method for DNA Isolation from Xylophagous Insects
Center for Ecosystems Research (CIEco), National Autonomous University of Mexico (UNAM), Morelia, Mexico
Multidisciplinary Center of Biotechnology Studies (CMEB), Michoacan University of San Nicolas of Hidalgo (UMSNH), Morelia, Mexico
* Author to whom correspondence should be addressed.
Received: 8 October 2010; in revised form: 22 November 2010 / Accepted: 30 November 2010 / Published: 7 December 2010
Abstract: Published methods to isolate DNA from insects are not always effective in xylophagous insects because they have high concentrations of phenolics and other secondary plant compounds in their digestive tracts. A simple, reliable and labor-effective cetyltrimethylammonium bromide-polyvinylpyrrolidone (CTAB-PVP) method for isolation of high quality DNA from xylophagous insects is described. This method was successfully applied to PCR and restriction analysis, indicating removal of common inhibitors. DNA isolated by the CTAB-PVP method could be used in most molecular analyses.
Keywords: Cerambycidae; DNA isolation; PCR; Oncideres albomarginata chamela; phenolics; restriction digestion; xylophagous insects
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MDPI and ACS Style
Calderón-Cortés, N.; Quesada, M.; Cano-Camacho, H.; Zavala-Páramo, G. A Simple and Rapid Method for DNA Isolation from Xylophagous Insects. Int. J. Mol. Sci. 2010, 11, 5056-5064.
Calderón-Cortés N, Quesada M, Cano-Camacho H, Zavala-Páramo G. A Simple and Rapid Method for DNA Isolation from Xylophagous Insects. International Journal of Molecular Sciences. 2010; 11(12):5056-5064.
Calderón-Cortés, Nancy; Quesada, Mauricio; Cano-Camacho, Horacio; Zavala-Páramo, Guadalupe. 2010. "A Simple and Rapid Method for DNA Isolation from Xylophagous Insects." Int. J. Mol. Sci. 11, no. 12: 5056-5064.