Next Article in Journal
Previous Article in Journal
Int. J. Mol. Sci. 2010, 11(12), 5056-5064; doi:10.3390/ijms11125056
Technical Note

A Simple and Rapid Method for DNA Isolation from Xylophagous Insects

1,* , 1
, 2
 and 2
Received: 8 October 2010; in revised form: 22 November 2010 / Accepted: 30 November 2010 / Published: 7 December 2010
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
View Full-Text   |   Download PDF [320 KB, updated 19 June 2014; original version uploaded 19 June 2014]
Abstract: Published methods to isolate DNA from insects are not always effective in xylophagous insects because they have high concentrations of phenolics and other secondary plant compounds in their digestive tracts. A simple, reliable and labor-effective cetyltrimethylammonium bromide-polyvinylpyrrolidone (CTAB-PVP) method for isolation of high quality DNA from xylophagous insects is described. This method was successfully applied to PCR and restriction analysis, indicating removal of common inhibitors. DNA isolated by the CTAB-PVP method could be used in most molecular analyses.
Keywords: Cerambycidae; DNA isolation; PCR; Oncideres albomarginata chamela; phenolics; restriction digestion; xylophagous insects Cerambycidae; DNA isolation; PCR; Oncideres albomarginata chamela; phenolics; restriction digestion; xylophagous insects
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Export to BibTeX |
EndNote


MDPI and ACS Style

Calderón-Cortés, N.; Quesada, M.; Cano-Camacho, H.; Zavala-Páramo, G. A Simple and Rapid Method for DNA Isolation from Xylophagous Insects. Int. J. Mol. Sci. 2010, 11, 5056-5064.

AMA Style

Calderón-Cortés N, Quesada M, Cano-Camacho H, Zavala-Páramo G. A Simple and Rapid Method for DNA Isolation from Xylophagous Insects. International Journal of Molecular Sciences. 2010; 11(12):5056-5064.

Chicago/Turabian Style

Calderón-Cortés, Nancy; Quesada, Mauricio; Cano-Camacho, Horacio; Zavala-Páramo, Guadalupe. 2010. "A Simple and Rapid Method for DNA Isolation from Xylophagous Insects." Int. J. Mol. Sci. 11, no. 12: 5056-5064.



Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert