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Int. J. Mol. Sci. 2010, 11(12), 5040-5055; doi:10.3390/ijms11125040
Review

Matrix-Assisted Laser Desorption/Ionization Imaging Mass Spectrometry

* ,
,
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Department of Molecular Anatomy, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka 431-3192, Japan
* Author to whom correspondence should be addressed.
Received: 10 November 2010 / Revised: 25 November 2010 / Accepted: 27 November 2010 / Published: 7 December 2010
(This article belongs to the Special Issue Biomarkers)
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Abstract

Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is a powerful tool that enables the simultaneous detection and identification of biomolecules in analytes. MALDI-imaging mass spectrometry (MALDI-IMS) is a two-dimensional MALDI-mass spectrometric technique used to visualize the spatial distribution of biomolecules without extraction, purification, separation, or labeling of biological samples. MALDI-IMS has revealed the characteristic distribution of several biomolecules, including proteins, peptides, amino acids, lipids, carbohydrates, and nucleotides, in various tissues. The versatility of MALDI-IMS has opened a new frontier in several fields such as medicine, agriculture, biology, pharmacology, and pathology. MALDI-IMS has a great potential for discovery of unknown biomarkers. In this review, we describe the methodology and applications of MALDI-IMS for biological samples.
Keywords: imaging mass spectrometry; matrix-assisted laser desorption/ionization mass spectrometry; biomarker; protein; lipids imaging mass spectrometry; matrix-assisted laser desorption/ionization mass spectrometry; biomarker; protein; lipids
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Zaima, N.; Hayasaka, T.; Goto-Inoue, N.; Setou, M. Matrix-Assisted Laser Desorption/Ionization Imaging Mass Spectrometry. Int. J. Mol. Sci. 2010, 11, 5040-5055.

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