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Matrix-Assisted Laser Desorption/Ionization Imaging Mass Spectrometry
Department of Molecular Anatomy, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka 431-3192, Japan
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Received: 10 November 2010; in revised form: 25 November 2010 / Accepted: 27 November 2010 / Published: 7 December 2010
(This article belongs to the Special Issue Biomarkers
Abstract: Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is a powerful tool that enables the simultaneous detection and identification of biomolecules in analytes. MALDI-imaging mass spectrometry (MALDI-IMS) is a two-dimensional MALDI-mass spectrometric technique used to visualize the spatial distribution of biomolecules without extraction, purification, separation, or labeling of biological samples. MALDI-IMS has revealed the characteristic distribution of several biomolecules, including proteins, peptides, amino acids, lipids, carbohydrates, and nucleotides, in various tissues. The versatility of MALDI-IMS has opened a new frontier in several fields such as medicine, agriculture, biology, pharmacology, and pathology. MALDI-IMS has a great potential for discovery of unknown biomarkers. In this review, we describe the methodology and applications of MALDI-IMS for biological samples.
Keywords: imaging mass spectrometry; matrix-assisted laser desorption/ionization mass spectrometry; biomarker; protein; lipids
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Zaima, N.; Hayasaka, T.; Goto-Inoue, N.; Setou, M. Matrix-Assisted Laser Desorption/Ionization Imaging Mass Spectrometry. Int. J. Mol. Sci. 2010, 11, 5040-5055.
Zaima N, Hayasaka T, Goto-Inoue N, Setou M. Matrix-Assisted Laser Desorption/Ionization Imaging Mass Spectrometry. International Journal of Molecular Sciences. 2010; 11(12):5040-5055.
Zaima, Nobuhiro; Hayasaka, Takahiro; Goto-Inoue, Naoko; Setou, Mitsutoshi. 2010. "Matrix-Assisted Laser Desorption/Ionization Imaging Mass Spectrometry." Int. J. Mol. Sci. 11, no. 12: 5040-5055.