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Int. J. Mol. Sci. 2010, 11(12), 5040-5055; doi:10.3390/ijms11125040
Review

Matrix-Assisted Laser Desorption/Ionization Imaging Mass Spectrometry

* ,
,
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Department of Molecular Anatomy, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka 431-3192, Japan
* Author to whom correspondence should be addressed.
Received: 10 November 2010 / Revised: 25 November 2010 / Accepted: 27 November 2010 / Published: 7 December 2010
(This article belongs to the Special Issue Biomarkers)
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Abstract

Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is a powerful tool that enables the simultaneous detection and identification of biomolecules in analytes. MALDI-imaging mass spectrometry (MALDI-IMS) is a two-dimensional MALDI-mass spectrometric technique used to visualize the spatial distribution of biomolecules without extraction, purification, separation, or labeling of biological samples. MALDI-IMS has revealed the characteristic distribution of several biomolecules, including proteins, peptides, amino acids, lipids, carbohydrates, and nucleotides, in various tissues. The versatility of MALDI-IMS has opened a new frontier in several fields such as medicine, agriculture, biology, pharmacology, and pathology. MALDI-IMS has a great potential for discovery of unknown biomarkers. In this review, we describe the methodology and applications of MALDI-IMS for biological samples.
Keywords: imaging mass spectrometry; matrix-assisted laser desorption/ionization mass spectrometry; biomarker; protein; lipids imaging mass spectrometry; matrix-assisted laser desorption/ionization mass spectrometry; biomarker; protein; lipids
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Zaima, N.; Hayasaka, T.; Goto-Inoue, N.; Setou, M. Matrix-Assisted Laser Desorption/Ionization Imaging Mass Spectrometry. Int. J. Mol. Sci. 2010, 11, 5040-5055.

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