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Int. J. Mol. Sci. 2010, 11(11), 4601-4617; doi:10.3390/ijms11114601
Published: 17 November 2010
Abstract: Millions of blood products are transfused every year; many lives are thus directly concerned by transfusion. The three main labile blood products used in transfusion are erythrocyte concentrates, platelet concentrates and fresh frozen plasma. Each of these products has to be stored according to its particular components. However, during storage, modifications or degradation of those components may occur, and are known as storage lesions. Thus, biomarker discovery of in vivo blood aging as well as in vitro labile blood products storage lesions is of high interest for the transfusion medicine community. Pre-analytical issues are of major importance in analyzing the various blood products during storage conditions as well as according to various protocols that are currently used in blood banks for their preparations. This paper will review key elements that have to be taken into account in the context of proteomic-based biomarker discovery applied to blood banking.
1. Blood Components Preparation and Storage
Whole blood transfusion is nowadays realized in very limited cases. Indeed, transfusion therapy now mainly relies on administration of the blood component really needed by the patients. The three main labile blood components used in transfusion therapy are erythrocyte concentrates (ECs), platelet concentrates (PCs) and fresh frozen plasma (FFP). These products can be obtained either by processing a whole blood donation (450–500 mL), which is the most common and simplest technique, or by apheresis where only the needed component is taken from the donor, the remainder been returned to the donor . This last technique is more expensive and requires a more consistent facility.
The standard procedure of blood products preparation from whole blood donation is as follows: once collected in plastic bags containing citrate phosphate dextrose (CPD) anticoagulant, whole blood is centrifuged in order to separate blood cells according to their size and density. Red blood cells (RBCs) settle, while plasma remains on the top. White blood cells and platelets (PLTs) form a “buffy coat” layer at the interface. Finally, the three components are distributed among the sterile inter-connected blood bags by applying a semi-automated pressure to the centrifuged bag containing the original whole blood donation.
In order to prevent various post-transfusion reactions and anti-HLA allo-immunisation, whole blood donation is impoverished in white cells by filtration (erythrocyte plasticity allows to pass through the membranes, contrary to white cells that are retained). Indeed, as leukocytes could be pathogens containing cells, blood products are systematically leukodepleted. A retrospective study has demonstrated that the rates of febrile nonhemolytic transfusion reactions (FNHTRs) are decreased by a factor of 1.7 and 4.1 after RBCs and PCs transfusion respectively, since the establishment of systematic prestorage leukodepletion .
Each component obtained from whole blood has optimal storage conditions, which permits to preserve its specific activities and functions. The temperature is a particularly important storage parameter regarding the viability and the quality of products intended for transfusion. Supplemented with an additive solution, generally a saline-adenine-glucose-mannitol (SAGM) solution, RBCs can be stored for up to 42 days from +2 °C to +6 °C, in order to preserve the functionalities of erythrocytes. On the contrary, PLT are stored from +20 °C to +24 °C up to 5 days, with sufficient agitation to permit a good oxygenation and to prevent platelet aggregation. Storage at room temperature promotes bacterial proliferation, and thus increases the risk of transmitted bacteria. The dilemma is that if platelets are transfused after refrigeration at 4 °C, they are rapidly cleared from the recipient circulation . Finally, as indicated by its name, FFP has to be frozen, for an optimal storage, at least at −25 °C, for up to 36 months.
These limits depend on local legislation, and depend on the additive solutions used in erythrocyte and platelet concentrates. These three labile blood products can be further differentially processed depending on demand for particular biomedical needs (γ-irradiation, washing and so on).
2. Blood Products Storage Lesions
Even under current optimal storage conditions, modifications and/or degradation of blood components occur in blood bags. These alterations, known as “storage lesions”, affect lifespan and quality of the stored blood products . Even though it is unknown if storage lesions are consequences of the natural aging of blood components, these lesions are well described in the literature.
2.1. Erythrocyte Concentrates Storage Lesions
First, some biochemical changes related to the energy metabolism occur. It appears that components such as ATP, which is necessary for multiple cellular processes, and 2,3-DPG, which plays an important role in oxygen release, rapidly decrease during the storage. ATP level is considerably diminished after 5 weeks of storage while the 2,3-DPG is almost null after 2 weeks of storage . The low concentration of 2,3-DPG increases hemoglobin affinity for oxygen, which cannot be delivered anymore. However, these levels are rapidly recovered in blood circulation after transfusion of the erythrocyte concentrate. It is also known that intracellular sodium and potassium levels are altered through storage. Indeed, Na+/K+ pumps are inactive at 4 °C, thus allowing high sodium influx and potassium loss .
Then, RBCs storage induces biomechanical changes. The erythrocytes rheological properties, such as shape, deformability, aggregability, and intracellular viscosity are altered during storage . All these changes impact the red blood cells ability to pass through microvessels, thus altering their oxygenation capacities. This is very problematic in blood banking, and neither SAGM nor phosphate-adenine-glucose-guanosin-saline-mannitol (PAGGSM), the two mostly used additive solutions, are described to prevent these storage lesions .
Finally, modifications also take place at the protein level. Indeed, erythrocytes are subjected to oxidative lesions, which result in protein oxidative modifications [10,11], hemichrome formation and Band 3 clustering. Storage-induced protein degradation appears to be greatly reduced when oxygen is removed and blood is stored under helium . Antonelou et al. have shown that erythrocyte proteins are less oxidized when RBCs are stored in CPD-SAGM compared to storage in CPD-Adenine . Mechanisms of oxidative damage along the development of storage lesions and investigations of the effect of blood anaerobic storage conditions have been recently described by Yoshida and Shevkoplyas .
Some aspects of RBC aging occurring in vivo and in vitro during storage are similar. For example, the increase of intracellular calcium level induces microvesiculation and externalization of negatively-charged membrane phospholipids (phosphatidylserine) . RBCs clearance from blood circulation is thought to be immunologically mediated. Several studies have led to the so-called Band 3 clustering model [16,17]. Oxidized hemoglobin compounds aggregate to form hemichromes that accumulate at the inner side of erythrocytes membrane, covalently bound with cytoskeletal proteins such as spectrin, thus inducing alterations of RBC deformability. Interaction of hemichromes with cytoplasmic domains of Band 3 leads to Band 3 clustering. This conformational change of the major erythrocyte membrane protein is recognized by naturally occurring anti-Band 3 auto-antibodies (nAbs) [18–20]. Paleari et al. have demonstrated that the number of these RBC-bound IgGs increases in old population enriched fractions (through Percoll density fractionation), as well as in some selected clinical cases of patients with altered RBC survival . These observations lead to the conclusion that RBC-bound IgG is a biomarker of aging. Hemicromes, Band 3 dimerization and erythrocyte recognition by nAbs are thus additional RBCs aging markers that can be investigated.
2.2. Platelet Concentrates Storage Lesions
Platelet Storage Lesions (PSL) consist in morphological changes, platelet activation, platelet proteolysis and platelet surface receptor expression [22,23]. Changes of platelet membrane glycoproteins are also reported in numerous papers [24–26].
The normal platelet discoid shape (also referred as resting shape) is found to be lost after 5 to 7 days of storage at 22 °C. At this storage time, mainly spherical or fragmented platelets remain. Granule release and platelet activation occur during PLT storage, as indicated by the accumulation of β-thromboglobulin and platelet factor 4 in the storage medium, and the increase in surface levels of P-selectin (CD62P), respectively.
In vitro, loss of aggregation functionality is also observed. There is a significantly storage-dependant decrease of platelet aggregation response to a number of agonists used alone, such as adenosine diphosphate (ADP), epinephrine, collagen and arachidonic acid . However, these aggregation agonists seem to have a synergistic action since pairs of them have been shown to restore platelet aggregation, even after five days of storage . Shapira et al. have also shown that platelet prothrombinase activity and membrane phosphatidylserine exposure are enhanced during PCs blood banking storage .
As blood components natural aging and storage lesions can directly affect cells, and also have implications at the protein level, the analysis of biomarkers of such phenomenon must be ruled out by important pre-analytical considerations.
3. Analysis of Blood Products Aging and Storage Lesions Biomarkers: Pre-Analytical Issues
3.1. Importance of Pre-Analytics in Biomarker Discovery Field
In order to be used in medicine, a biomarker has to be both sensitive and specific. Indeed, a biomarker of a given physiological state must allow the identification of this state (sensitivity) and must not be relevant to another physiological state (specificity). However, only few of them meet all criteria making an efficient biomarker suitable for clinical uses.
In the field of biomarker discovery, an important issue is to control sample preparation steps and pre-analytical factors. Specificities of a biomarker have to be the same everywhere following the same protocol. For that reason, the standardization of procedure controlling pre-analytical steps (sampling, conservation and preparation) has to be elaborated.
As discussed before, the three main labile products are differentially processed and stored in order to maintain their viability and quality for transfusion therapy. Standards are published by competent authorities in each country. However, even if the procedure is similar, there are still minor changes applied worldwide. Indeed, blood labile products have to meet definite standards, whatever the way of production and the material used. For example, quality of platelet concentrates varies according to the platelet additive solution used .
3.2. Proteomics in Blood Transfusion
3.2.1. Proteomic Tools for Biomarker Discovery
Proteomics has been largely used in the field of biomarker discovery [30,31] as well as in transfusion medicine [32,33] since proteins are the effective form of genes, and are thus more able to inform about the physiological state. Indeed, contrary to the genome, the proteome composition fluctuates during time, changes among cell population and is physiological state-dependent.
Clinical proteomics is a major asset for diagnosis, prognosis or evolutionary biomarkers. Varieties of improved proteomic technologies allow separation, identification and quantification of proteins, here potential biomarkers. Gel-based technologies (two-dimensional gel electrophoresis, 2DE, and two-dimensional differential gel electrophoresis, 2D-DIGE), as well as chromatographic techniques (e.g., reverse phase liquid chromatography, RPLC, and strong cation exchange liquid chromatography, SCX-LC) have been developed in order to fractionate proteins or peptides from complex samples. Then, proteins can be analyzed by mass spectrometry (MS) consisting in the ionization of proteins or peptides in order to identify them according to their mass-to-charge ratio (m/z). The two commonly used sources of ionization are matrix-assisted laser desorption/ionization (MALDI) and electrospray ionization (ESI). MALDI source is preferentially combined with time-of-flight (TOF) analyzer, whereas the ESI source is coupled with quadrupole (Q), ion trap (IT), fourier transform ion cyclotron resonance (FTICR), Orbitrap or combinations of these analyzers. Peptide mass fingerprinting (PMF) or de novo sequencing by tandem mass spectrometry (MS/MS or MS2), combined with powerful bioinformatic tools allow protein identification by database interrogation. Protein quantitation [34,35] can be achieved thanks to diverse treatments before or after the sampling step  (metabolic stable isotope labeling of amino acids in cell culture, SILAC , and chemical addition of isotope-coded affinity tags, ICAT , or isobaric tags for relative and absolute quantitation, iTRAQ ), by spiking samples before MS analysis with known amount of an isotopically labeled analyte (absolute quantitation of proteins, AQUA ) and even by recently developed label-free methods, based on MS or chromatography data-processing (spectral counting  or total ion current, TIC, integration ).
Surface enhanced lazer desorption/ionization time-of-flight (SELDI-TOF), also known as ProteinChip Array technology, is widely used in biomarker discovery [43,44]. A solid-phase chromatographic surface allows sample decomplexification according to chemical or biochemical properties. Users can design their own chromatographic surface to target specific protein population. Bound proteins are then ionized and analyzed as in classical MALDI-TOF MS analysis. However, SELDI-TOF MS analysis does not lead to protein identification, but only gives a mass list of proteins showing relative abundance differences between samples. Once targeted, these proteins have to be further purified then identified by standard mass spectrometry. Caputo et al. have developed such kind of methods for protein identification on ProteinChip surfaces, i.e., on-chip digestion and MS/MS sequencing by hybrid quadrupole-TOF .
Recent advances in all these proteomic techniques have improved reproducibility, but the problem of inter-laboratory reproducibility still subsists. To ensure this reproducibility, a control of the initial state of samples, with standardization of sample collection, storage and preparation conditions, is required. According to Lehmann et al. , the following pre-analytical steps have to be controlled: the sampling procedure and the type of container, the temperature and the time before transport (if transport needed), the time between sample collection and sample processing, the process itself (centrifugation, aliquoting, type of secondary container), the storage (temperature and duration) and the pre-treatment just before proteomic analysis.
The general complexity of a proteome, and particularly the huge dynamic range of protein abundance composing the blood proteome, makes necessary the fractionation of blood samples before proteomic analysis, by depletion or fractionation processes. Then, other sample preparation can be required depending on the type of analysis to be performed, and cannot be standardized to all biomarker discovery studies.
3.2.2. Involvement of Proteomics in Blood Transfusion
22.214.171.124. Erythrocyte Proteome Investigations
Red blood cell is an abundant and easily obtained biological material having the particularity to be nucleus- and organelle-free. These characteristics have made of it a model of choice for membrane studies. Nowadays, thanks to development of proteomic tools and technologies, erythrocyte proteome begins to be well documented. Several major studies about RBC proteome have been published last decade, showing a great improvement in terms of number of identified proteins, in cytosolic as well as membrane extracts.
In 2002, a first study combining one dimensional (sodium dodecyl sulphate polyacrylamide gel electrophoresis, SDS-PAGE) and 2D electrophoresis, allows identification of 84 unique proteins from ghost preparation by MALDI-TOF MS analysis. Classic SDS-PAGE reveals 25 proteins of high hydrophobicity and high molecular weight, undetectable with 2DE . Two years later, the use of LC-MS has enabled Goodman and coworkers to identify 181 proteins after tryptic digestion of both cytoplasmic and membrane preparations . Then, Pasini et al. have increased the number of identified proteins to 566 (314 membrane-associated proteins and 252 cytoplasmic proteins), combining high-accuracy and high-sensitivity protein identification technologies to analyze trypsin-digested differentially extracted proteins from cytoplasmic and membrane fractions. They have tested different extraction methods leading to differences in number of identified proteins. This number also appeared to vary according to interrogated database . In 2008, the peptide ligand library technology  made possible the exploration of red blood cell hidden proteome by reducing the erythrocyte dynamic protein concentration range, which permits Roux-Dalvai et al. to identify 1578 soluble proteins by nano-LC-LTQ-Orbitrap MS/MS analysis . A more recent study reveals 222 identified proteins. Even though this number may appear low in comparison with other studies, the authors have set up an interesting hemoglobin depletion strategy, derived from the existing HemogloBind™ reagent . In 2010, van Gestel et al. have explored RBC membrane proteome combining blue native protein separation (BN/SDS-PAGE), CyDyes labeling-based quantitation and LC-MS/MS identification . This allowed them to identify 524 proteins, of which 155 are membrane proteins. The CyDyes labeling led to quantitation of a 40% decrease in spectrin levels in erythrocyte membrane fraction of a patient presenting RBC membrane disorder. This interesting approach thus seems to be applicable to biomarker discovery. Lately, efforts have been made in the establishment of interactome and network maps, as reported by Goodman et al.  and updated by D’Alessandro et al. .
126.96.36.199. Platelet Proteomic Analyses
Platelet quality was usually determined from measurable characteristics such as pH, platelet morphology and hypotonic shock responses. Protein analysis of platelets during storage was first achieved in the end of the 1980s by identifying variation in actin [56,57]. However, actin polymerization is linked to the method used in the preparation of PCs and it has been shown that this process is partially reversible after 1 day of storage , which is highlighting the implication of pre-analytics in biomarker discovery. Since the introduction of proteomics, new tools have been brought in order to characterize platelet storage lesions. As explained previously, lesions occur during storage of PCs and can alter several proteins and mechanisms. The proteome analysis of platelets during the period of storage may reveal different biomarkers, potentially important for improving platelet quality . Proteomic analysis of platelets in vivo or during storage was achieved at the beginning of the last decade [59,60]. Using gel-based proteomics, different groups identified several hundreds to thousands of PLT proteins [61–63]. Based on peptidic-centric methods, 641 proteins were reported by Gevaert and coworkers, including hydrophobic membrane proteins . Later, different studies have focused on changes during storage periods, typically between a 1-day and a 7-day storage. Thiele et al. have shown by DIGE and MS that 97% of cytosolic proteins do not change over a 9-day storage period . However, in the 3 remaining percent, they highlighted that, for instance, spetin 2 and gelsolin show changes in 2DE. They claimed that these proteins, affected during apoptosis, may be suitable markers for platelet alteration during storage. Using a similar approach but focusing on supernatant, it has been also shown that levels of proteins increase during storage, which may have some implications for transfusion recipients . More recently, Devine and coworkers have used complementary proteomic methods (2DE, DIGE, iTRAQ and ICAT) to investigate protein changes between days 1 and 7 of PLT storage . Hence, 503 proteins changed concentration was reported thus completing the PLT proteome.
Beyond these analyses, the impact of such potentially biomarkers has to be integrated within molecular mechanisms leading to platelet storage. As explained by Schubert and Devine, biological interpretation of proteomic data has to be taken into account . A comprehensive view of different mechanisms related to biomarkers may be required to understand how to improve platelet quality. Even though such an approach is far to be completed nowadays, changes in molecular mechanisms have been described. Schubert et al. have identified 12 proteins connected in one potential signaling pathway underlying storage lesion development . They have shown that PI3-kinase-dependent Rap1 activation leads to integrin αIIbβ3 activation and PLT degranulation. In addition, a PI3-kinase inhibitor incubated with PLT for 7 days plays a role in Rap1 activation and seems to improve PLT integrity and quality during storage. A global view, including protein-protein interactions , is one of the tools able to provide a wide picture of the storage lesions and mechanisms involved during such modifications.
All these studies have potential influence on the future of platelet quality and safety during storage, and of course, as it is explained within this review, pre-analytic considerations are inherent to these studies.
3.3. Blood Proteomics Pre-Analytical Considerations
All these blood-related studies can be impacted by pre-analytics. From sample collection to proteomic analysis, all manipulation steps are source of pre-analytical variation. The main pre-analytical considerations to take into account are resumed in Table 1.
3.3.1. Sample Collection
Blood donation conditions such as the donor position, the time of tourniquet application, and the needle bore size are important. In upright position, blood macromolecular component rates can be increased, due to a shift in body water from intravascular to interstitial compartment (macromolecules cannot pass through blood vessels and are thus concentrated) . The same effect can be noticed if the tourniquet application exceeds one minute. Indeed, proteins and macromolecules can thus accumulate upstream the tourniquet and be detected at a higher rate than normal by this effect of concentration. Moreover, Cengiz et al. have shown in a recent study that erythrocyte deformability is still altered up to 180 seconds after tourniquet removal, and erythrocyte aggregation is increased up to 30 seconds after tourniquet removal . They also confirmed that tourniquet application induces leukocytes activation.
Concerning the needle bore size, too thin needle can induce hemolysis, thus free hemoglobin and other protein concentrations increase in serum/plasma. It is not problematic when working with blood products obtained from whole blood donation because in case of large blood drawn volume, largest needles are used (16 to 19-gauge needles). In routine venipuncture, smaller blood volumes are drawn and patient comfort is of high importance, thus thinner needles are chosen (21 to 23-gauge needles). However, too thin needles (25-gauge needles) should be avoided since it can induce pre-analytical variability (hemolysis), leading to false interpretation of test results [72,73].
3.3.2. Sample Container Type
All containers have to be tested for a possible interference with analyses to be performed. Indeed, containers can salt out plastic components, which can be detected in analysis by mass spectrometry. The presence of polymers in an MS analysis induces effect of signal suppression: the high amount of ionized polymer saturates the detector, and the other sample minority components ions cannot be detected anymore. In 2004, Drake et al. have demonstrated the presence of polymers on mass spectra from extracts of saline solution incubated in different blood sample containers, from two different manufacturers .
Investigating serum/plasma proteomes, Hsieh et al. have shown that there was no significant difference between samples collected in glass red-top tubes and samples collected in serum separator tiger-top tubes . However, comparing the nature of the anticoagulant used, they have found high significant differences in plasma protein profiles. Most common anticoagulants are EDTA, heparin and sodium citrate. Their different action ways lead to these differences in proteome composition. Moreover, they are also known to interfere with subsequent analysis. EDTA is known to interfere in enzymatic activity assays, whereas heparin is not advised for some ELISA-based assay kit.
3.3.3. Sample Processing and Handling
First of all, the time between blood donation and preparation of the labile products is of high importance. Banks et al. have shown in 2005 that this duration induces differences in SELDI-TOF profiles of plasma collected with different anticoagulants and serum samples .
A study of West-Nielson et al., based on principal component analysis (PCA) of MALDI-TOF spectra, reveals that there was no significant difference between spectra of serum from blood left to clot up to 24 h at 4 °C and from blood left to clot up to 4 h at 24 °C . However, when blood is left to clot for longer times at 24 °C (8 h to 24 h), clearly changes appear in mass spectra, which was confirmed later . In 2007, Timms et al. have shown that both transport time and temperature play a role on SELDI-TOF spectra profiles of sera .
Studying RBC microparticles, Rubin et al. have encountered some pre-analytical caveats. Indeed, our group has shown that sample processing temperature and vortexing duration affect microparticles counting . Dealing with centrifugation force (1500 or 3000 × g) and duration (15 vs. 30 minutes), serum proteome profiles do not appear to be affected .
Protein degradations, such as fragmentation by proteolysis, and protein modification, such as oxidation, have been described as storage lesions (see part 2). However, these alterations can be induced during sample manipulation, and cannot be differentiated from storage lesions. The addition of protease inhibitors to prevent handling-related proteolysis is thus of high importance. If protease inhibitors are omitted, it has been shown that low molecular weight compounds appear and accumulate during sample preparation . It is necessary to prevent proteolytic cleavages because generated peptides could thus be mistaken for potential biomarker. Indeed, analyzing peptidome variations of pathological sera, Davis et al. have shown that modifications in low molecular weight compounds reveal disease-induced hemostatic dysregulation rather than direct protease activity of a given pathology . Indirectly pathologically-induced peptidome variations are not specific enough to be considered as good biomarkers and have thus to be avoided by use of protease inhibitors, even if it is not known whether all protease activities are repressed.
RBCs possess a set of enzymes responsible for antioxidant defense (catalase, superoxide dismutase, peroxiredoxins and glutathione peroxidase). However, these enzymes can be inactivated by excess of reactive oxygen species (ROS), and additional protein oxidation can thus occur during sample processing. In order to prevent this phenomenon, particularly prior to cystein oxidation assessment in stored RBC redox proteomic studies, cell-permeable cystein-specific reagents, such as iodoacetamide (IAA) or N-ethylmaleimide (NEM) alkylating agents, are commonly used [82,83].
3.3.4. Sample Storage
Storage conditions are important to preserve quality of labile blood products and to ensure the efficiency of transfusion therapy. Thus, it is known that erythrocyte concentrates are preferentially stored at 4 °C whereas platelet concentrates are stored at 22 °C to prevent cold activation of platelets. Finally, fresh frozen plasma is stored at −25 °C at least.
Thus, whole blood storage cannot ensure optimal preservation of each blood components. In labile blood products aging field, samples are ECs, PCs or FFPs, and are stored according to blood banking legislation. In pathological-case biomarker research, samples are either serum or plasma, and are not subject to any storage legislation. Several studies aimed at investigating the reproducibility of analysis after storage at −20/−30 °C or −80 °C, and after freeze-thaw cycles [75,84,85]. It appears that storage at −20 °C instead of the recommended −80 °C storage does not induce loss of information. Samples stored at −20/−30 °C can thus be used for biomarker discovery .
Some proteins have been shown to be highly temperature-sensitive: cryoproteins. Described for the first time in the 1930’s by Wintrobe  as myeloma’s patient sera precipitating at cold temperature, cryoproteins are serum or plasma proteins having the property to precipitate below the physiological temperature. Most of the time composed of immunoglobulins (mainly IgM) these precipitates are called cryoglobulins (CG), and are classified under three categories according to the universally adopted classification of Brouet . Other proteins have this particularity to precipitate at low temperature, as cryofibrinogen (CF) (for instance, see the study on clinical cases of patients with either CF alone or combined CF plus CG ) and cold agglutinins. Proteomic techniques are well adapted to determine the protein composition of cryoprecipitates . Cryoproteins are potential biomarkers, and pre-analytical precautions have to be taken for their investigation. Indeed, blood must be sampled in anticoagulant-free, 37 °C pre-heated container, handled and centrifuged at 37 °C, in order to prevent cryoprotein elimination by precipitation before analysis.
Pre-analytics is of very high importance for all kind of test, assay and discovery field, and more importantly in biomarker discovery field, due to the direct clinical implications. Uncontrolled pre-analytical parameters may lead to false interpretation of results.
In clinical proteomics, reproducibility is indispensable and is straightly correlated with pre-analytical considerations. Standardization of sample harvesting, process, and storage has to be set up in order to minimize pre-analytical variations. Biomarker discovery is such a complicated field that there is no time to waste in wondering if an observed protein variation is due to different physiological states or simply to differences in sample handling or storage. Many small-scale standardized protocols exist in laboratories and lots of pre-analytical parameters have been investigated so far. Time is now about setting up worldwide-scale standardization of sample handling, process and storage.
The human proteome organization (HUPO) has set up the plasma proteome project (HPPP), in order to perform a comprehensive analysis of plasma and serum protein constituents in people. A specimen collection and handling committee (SCHC) was created to evaluate pre-analytical variables that can potentially impact experiment results. In this purpose, Rai et al. have conducted in 2005 a proteomic study, comparing serum and plasma analyses, evaluating storage and handling conditions as well as the use of protease inhibitors . They were thus able to present general recommendations helping researchers to set up more robust plasma proteome studies. This is an important step towards the establishment of standardized protocols for proteome studies, which is primordial in the biomarker discovery field.
This kind of initiative is important for the transfusion medicine community and for the whole clinical medicine community as well. Reviews about importance of the pre-analytics in many fields are regularly published nowadays, which means that procedures standardization is a real contemporary thought.
We would like to thank the Service de Transfusion Sanguine de la Croix Rouge Suisse for the grant supporting this work.
- Council of Europe. Guide to the Preparation, Use and Quality Assurance of Blood Components. Recommendation n° R (95) 15 on the Preparation, Use and Quality Assurance of Blood Components, 14th ed ed.; Council of Europe Press: Strasbourg, France, 2008.
- Yazer, MH; Podlosky, L; Clarke, G; Nahirniak, SM. The effect of prestorage WBC reduction on the rates of febrile nonhemolytic transfusion reactions to platelet concentrates and RBC. Transfusion 2004, 44, 10–15.
- Snyder, EL; Rinder, HM. Platelet storage-time to come in from the cold? N. Engl. J. Med 2003, 348, 2032–2033.
- Zubair, AC. Clinical impact of blood storage lesions. Am. J. Hematol 2010, 85, 117–122.
- van de Watering, LMG; Brand, A. Effects of storage of red cells. Transfus. Med. Hemother 2008, 35, 359–367.
- Kor, DJ; van Buskirk, CM; Gajic, O. Red blood cell storage lesion. Bosn. J. Basic Med. Sci 2009, 9, S21–S27.
- Bennett-Guerrero, E; Veldman, TH; Doctor, A; Telen, MJ; Ortel, TL; Reid, TS; Mulherin, MA; Zhu, HM; Buck, RD; Califf, RM; McMahon, TJ. Evolution of adverse changes in stored RBCs. Proc. Natl. Acad. Sci. USA 2007, 104, 17063–17068.
- Berezina, TL; Zaets, SB; Morgan, C; Spillert, CR; Kamiyama, M; Spolarics, Z; Deitch, EA; Machiedo, GW. Influence of storage on red blood cell rheological properties. J. Surg. Res 2002, 102, 6–12.
- Zehnder, L; Schulzki, T; Goede, JS; Hayes, J; Reinhart, WH. Erythrocyte storage in hypertonic (SAGM) or isotonic (PAGGSM) conservation medium: Influence on cell properties. Vox Sang 2008, 95, 280–287.
- Kriebardis, AG; Antonelou, MH; Stamoulis, KE; Economou-Petersen, E; Margaritis, LH; Papassideri, IS. Progressive oxidation of cytoskeletal proteins and accumulation of denatured hemoglobin in stored red cells. J. Cell. Mol. Med 2007, 11, 148–155.
- Kanias, T; Acker, JP. Biopreservation of red blood cells - the struggle with hemoglobin oxidation. FEBS J 2010, 277, 343–356.
- D’Amici, GM; Rinalducci, S; Zolla, L. Proteomic analysis of RBC membrane protein degradation during blood storage. J. Proteome Res 2007, 6, 3242–3255.
- Antonelou, MH; Kriebardis, AG; Stamoulis, KE; Economou-Petersen, E; Margaritis, LH; Papassideri, IS. Red blood cell aging markers during storage in citrate-phosphate-dextrose-saline-adenine-glucose-mannitol. Transfusion 2010, 50, 376–389.
- Yoshida, T; Shevkoplyas, SS. Anaerobic storage of red blood cells. Blood Transfus 2010, 8, 220–236.
- Tissot, J-D; Rubin, O; Canellini, G. Analysis and clinical relevance of microparticles from red blood cells. Curr. Opin. Hematol 2010, 17, 571–577.
- Kay, MM; Wyant, T; Goodman, J. Autoantibodies to band 3 during aging and disease and aging interventions. Ann. N. Y. Acad. Sci 1994, 719, 419–447.
- Hornig, R; Lutz, HU. Band 3 protein clustering on human erythrocytes promotes binding of naturally occurring anti-band 3 and anti-spectrin antibodies. Exp. Gerontol 2000, 35, 1025–1044.
- Kay, MM; Goodman, SR; Sorensen, K; Whitfield, CF; Wong, P; Zaki, L; Rudloff, V. Senescent cell antigen is immunologically related to band 3. Proc. Natl. Acad. Sci. USA 1983, 80, 1631–1635.
- Kay, MM. Localization of senescent cell antigen on band 3. Proc. Natl. Acad. Sci. USA 1984, 81, 5753–5757.
- Kay, MM; Flowers, N; Goodman, J; Bosman, G. Alteration in membrane protein band 3 associated with accelerated erythrocyte aging. Proc. Natl. Acad. Sci. USA 1989, 86, 5834–5838.
- Paleari, R; Ceriotti, F; Azzario, F; Maccioni, L; Galanello, R; Mosca, A. Experiences in the measurement of RBC-bound IgG as markers of cell age. Bioelectrochemistry 2004, 62, 175–179.
- Seghatchian, J; Krailadsiri, P. The platelet storage lesion. Transf. Med. Rev 1997, 11, 130–144.
- Shrivastava, M. The platelet storage lesion. Transfus. Apher. Sci 2009, 41, 105–113.
- George, JN; Pickett, EB; Heinz, R. Platelet membrane glycoprotein changes during the preparation and storage of platelet concentrates. Transfusion 1988, 28, 123–126.
- Bessos, H; Seghatchian, MJ; Cutts, M; Murphy, WG. Glycoprotein-IB and glycoprotein-IIB/IIIA in the quality assessment of platelet concentrates during storage. Blood Coagul. Fibrinolysis 1992, 3, 633–636.
- Jaremo, P; Rubachdahlberg, E; Solum, NO. Correlation of light transmission changes to changes of platelet glycoprotein-IB during storage of platelet concentrates. Thromb. Res 1993, 69, 467–477.
- Diminno, G; Silver, MJ; Murphy, S. Stored human-platelets retain full aggregation potential in response to pairs of aggregating agents. Blood 1982, 59, 563–568.
- Shapira, S; Friedman, Z; Shapiro, H; Presseizen, K; Radnay, J; Ellis, MH. The effect of storage on the expression of platelet membrane phosphatidylserine and the subsequent impact on the coagulant function of stored platelets. Transfusion 2000, 40, 1257–1263.
- van der Meer, PF; Kerkhoffs, JL; Curvers, J; Scharenberg, J; de Korte, D; Brand, A; de Wildt-Eggen, J. In vitro comparison of platelet storage in plasma and in four platelet additive solutions, and the effect of pathogen reduction: A proposal for an in vitro rating system. Vox Sang 2010, 98, 517–524.
- Hale, JE; Gelfanova, V; Ludwig, JR; Knierman, MD. Application of proteomics for discovery of protein biomarkers. Brief. Funct. Genomic Proteomic 2003, 2, 185–193.
- Aebersold, R; Anderson, L; Caprioli, R; Druker, B; Hartwell, L; Smith, R. Perspective: A program to improve protein biomarker discovery for cancer. J. Proteome Res 2005, 4, 1104–1109.
- Thadikkaran, L; Siegenthaler, MA; Crettaz, D; Queloz, PA; Schneider, P; Tissot, JD. Recent advances in blood-related proteomics. Proteomics 2005, 5, 3019–3034.
- Queloz, PA; Thadikkaran, L; Crettaz, D; Rossier, JS; Barelli, S; Tissot, JD. Proteomics and transfusion medicine: Future perspectives. Proteomics 2006, 6, 5605–5614.
- Simpson, KL; Whetton, AD; Dive, C. Quantitative mass spectrometry-based techniques for clinical use: Biomarker identification and quantification. J. Chromatogr. B 2009, 877, 1240–1249.
- Anderson, NL; Anderson, NG; Pearson, TW; Borchers, CH; Paulovich, AG; Patterson, SD; Gillette, M; Aebersold, R; Carr, SA. A human proteome detection and quantitation project. Mol. Cell. Proteomics 2009, 8, 883–886.
- Kline, KG; Sussman, MR. Protein quantitation using isotope-assisted mass spectrometry. Ann. Rev. Biophys 2010, 39, 291–308.
- Ong, SE; Blagoev, B; Kratchmarova, I; Kristensen, DB; Steen, H; Pandey, A; Mann, M. Stable isotope labeling by amino acids in cell culture, SILAC, as a simple and accurate approach to expression proteomics. Mol. Cell. Proteomics 2002, 1, 376–386.
- Gygi, SP; Rist, B; Gerber, SA; Turecek, F; Gelb, MH; Aebersold, R. Quantitative analysis of complex protein mixtures using isotope-coded affinity tags. Nat. Biotechnol 1999, 17, 994–999.
- Ross, PL; Huang, YN; Marchese, JN; Williamson, B; Parker, K; Hattan, S; Khainovski, N; Pillai, S; Dey, S; Daniels, S; Purkayastha, S; Juhasz, P; Martin, S; Bartlet-Jones, M; He, F; Jacobson, A; Pappin, DJ. Multiplexed protein quantitation in Saccharomyces cerevisiae using amine-reactive isobaric tagging reagents. Mol. Cell. Proteomics 2004, 3, 1154–1169.
- Gerber, SA; Rush, J; Stemman, O; Kirschner, MW; Gygi, SP. Absolute quantification of proteins and phosphoproteins from cell lysates by tandem MS. Proc. Natl. Acad. Sci. USA 2003, 100, 6940–6945.
- Liu, H; Sadygov, RG; Yates, JR, III. A model for random sampling and estimation of relative protein abundance in shotgun proteomics. Anal. Chem 2004, 76, 4193–4201.
- Asara, JM; Christofk, HR; Freimark, LM; Cantley, LC. A label-free quantification method by MS/MS TIC compared to SILAC and spectral counting in a proteomics screen. Proteomics 2008, 8, 994–999.
- Issaq, HJ; Veenstra, TD; Conrads, TP; Felschow, D. The SELDI-TOF MS approach to proteomics: Protein profiling and biomarker identification. Biochem. Biophys. Res. Commun 2002, 292, 587–592.
- De Bock, M; de Seny, D; Meuwis, MA; Chapelle, JP; Louis, E; Malaise, M; Merville, MP; Fillet, M. Challenges for biomarker discovery in body fluids using SELDI-TOF-MS. J. Biomed. Biotechnol 2010, 2010, 906082.
- Caputo, E; Moharram, R; Martin, BM. Methods for on-chip protein analysis. Anal. Biochem 2003, 321, 116–124.
- Lehmann, S; Roche, S; Allory, Y; Barthelaix, A; Beaudeux, J-L; Berger, F; Betsou, F; Borg, J; Dupuy, A; Garin, J; Quillard, M; Lizard, G; Peoc’h, K; Riviere, M; Ducoroy, P. Recommandations préanalytiques pour les analyses de protéomique clinique des fluides biologiques. Ann. Biol. Clin 2009, 67, 629–639.
- Low, TY; Seow, TK; Chung, MCM. Separation of human erythrocyte membrane associated proteins with one-dimensional and two-dimensional gel electrophoresis followed by identification with matrix-assisted laser desorption/ionization-time of flight mass spectrometry. Proteomics 2002, 2, 1229–1239.
- Kakhniashvili, DG; Bulla, LA; Goodman, SR. The human erythrocyte proteome - Analysis by ion trap mass spectrometry. Mol. Cell. Proteomics 2004, 3, 501–509.
- Pasini, EM; Kirkegaard, M; Mortensen, P; Lutz, HU; Thomas, AW; Mann, M. In-depth analysis of the membrane and cytosolic proteome of red blood cells. Blood 2006, 108, 791–801.
- Boschetti, E; Righetti, PG. The art of observing rare protein species in proteomes with peptide ligand libraries. Proteomics 2009, 9, 1492–1510.
- Roux-Dalvai, F; de Peredo, AG; Simo, C; Guerrier, L; Bouyssiee, D; Zanella, A; Citterio, A; Burlet-Schiltz, O; Boschetti, E; Righetti, PG; Monsarrat, B. Extensive analysis of the cytoplasmic proteome of human erythrocytes using the peptide ligand library technology and advanced mass spectrometry. Mol. Cell. Proteomics 2008, 7, 2254–2269.
- Alvarez-Llamas, G; de la Cuesta, F; Barderas, MG; Darde, VM; Zubiri, I; Caramelo, C; Vivanco, F. A novel methodology for the analysis of membrane and cytosolic sub-proteomes of erythrocytes by 2-DE. Electrophoresis 2009, 30, 4095–4108.
- van Gestel, RA; van Solinge, WW; van der Toorn, HW; Rijksen, G; Heck, AJ; van Wijk, R; Slijper, M. Quantitative erythrocyte membrane proteome analysis with Blue-native/SDS PAGE. J. Proteomics 2010, 73, 456–465.
- Goodman, SR; Kurdia, A; Ammann, L; Kakhniashvili, D; Daescu, O. The human red blood cell proteome and interactome. Exp. Biol. Med. (Maywood) 2007, 232, 1391–1408.
- D’Alessandro, A; Righetti, PG; Zoha, L. The red blood cell proteome and interactome: An update. J. Proteome Res 2010, 9, 144–163.
- Snyder, EL; Dunn, BE; Giometti, CS; Napychank, PA; Tandon, NN; Ferri, PM; Hofmann, JP. Protein-changes occurring during storage of platelet concentrates - a two-dimensional gel-electrophoretic analysis. Transfusion 1987, 27, 335–341.
- Snyder, EL; Horne, WC; Napychank, P; Heinemann, FS; Dunn, B. Calcium-dependent proteolysis of actin during storage of platelet concentrates. Blood 1989, 73, 1380–1385.
- Estebanell, E; Diaz-Ricart, M; Lozano, M; Mazzara, R; Escolar, G; Ordinas, A. Cytoskeletal reorganization after preparation of platelet concentrates, using the buffy coat method, and during their storage. Haematologica 1998, 83, 112–117.
- Schubert, P; Devine, DV. Proteomics meets blood banking: Identification of protein targets for the improvement of platelet quality. J. Proteomics 2010, 73, 436–444.
- Egidi, MG; D’Alessandro, A; Mandarello, G; Zolla, L. Troubelshooting in platelet storage temperature and new perspectives through peoteomics. Blood Transfus 2010, 8, s73–s81.
- Marcus, K; Immler, D; Sternberger, J; Meyer, HE. Identification of platelet proteins separated by two-dimensional gel electrophoresis and analyzed by matrix assisted laser desorption/ioniztion-time of flight-mass spectrometry and detection of tyrosine-phosphorylated proteins. Electrophoresis 2000, 21, 2622–2636.
- O’Neill, EE; Brock, CJ; von Kriegsheim, AF; Pearce, AC; Dwek, RA; Watson, SP; Hebestreit, HF. Towards complete analysis of the platelet proteome. Proteomics 2002, 2, 288–305.
- Garcia, A; Prabhakar, S; Brock, CJ; Pearce, AC; Dwek, RA; Watson, SP; Hebestreit, HF; Zitzmann, N. Extensive analysis of the human platelet proteome by two-dimensional gel electrophoresis and mass spectrometry. Proteomics 2004, 4, 656–668.
- Martens, L; van Damme, P; van Damme, J; Staes, A; Timmerman, E; Ghesquiere, B; Thomas, GR; Vandekerckhove, J; Gevaert, K. The human platelet proteome mapped by peptide-centric proteomics: A functional protein profile. Proteomics 2005, 5, 3193–3204.
- Thiele, T; Steil, L; Gebhard, S; Scharf, C; Hammer, E; Brigulla, M; Lubenow, N; Clemetson, KJ; Volker, U; Greinacher, A. Profiling of alterations in platelet proteins during storage of platelet concentrates. Transfusion 2007, 47, 1221–1233.
- Glenister, KM; Payne, KA; Sparrow, RL. Proteomic analysis of supernatant from pooled buffy-coat platelet concentrates throughout 7-day storage. Transfusion 2008, 48, 99–107.
- Thon, JN; Schubert, P; Duguay, M; Serrano, K; Lin, SJ; Kast, J; Devine, DV. Comprehensive proteomic analysis of protein changes during platelet storage requires complementary proteomic approaches. Transfusion 2008, 48, 425–435.
- Schubert, P; Thon, JN; Walsh, GM; Chen, CHI; Moore, ED; Devine, DV; Kast, J. A signaling pathway contributing to platelet storage lesion development: Targeting PI3-kinase-dependent Rap1 activation slows storage-induced platelet deterioration. Transfusion 2009, 49, 1944–1955.
- Qureshi, AH; Chaoji, V; Maiguel, D; Faridi, MH; Barth, CJ; Salem, SM; Singhal, M; Stoub, D; Krastins, B; Ogihara, M; Zaki, MJ; Gupta, V. Proteomic and phospho-proteomic profile of human platelets in basal, resting state: Insights into integrin signaling. PLoS One 2009, 4, e7627.
- Maw, GJ; Mackenzie, IL; Taylor, NA. Redistribution of body fluids during postural manipulations. Acta Physiol. Scand 1995, 155, 157–163.
- Cengiz, M; Ulker, P; Meiselman, HJ; Baskurt, OK. Influence of tourniquet application on venous blood sampling for serum chemistry, hematological parameters, leukocyte activation and erythrocyte mechanical properties. Clin. Chem. Lab. Med 2009, 47, 769–776.
- Ernst, DJ; Ernst, C. Phlebotomy tools of the trade. Home Healthc. Nurse 2002, 20, 151–153.
- Lippi, G; Salvagno, GL; Montagnana, M; Brocco, G; Guidi, GC. Influence of the needle bore size used for collecting venous blood samples on routine clinical chemistry testing. Clin. Chem. Lab. Med 2006, 44, 1009–1014.
- Drake, SK; Bowen, RAR; Remaley, AT; Hortin, GL. Potential interferences from blood collection tubes in mass spectrometric analyses of serum polypeptides. Clin. Chem 2004, 50, 2398–2401.
- Hsieh, SY; Chen, RK; Pan, YH; Lee, HL. Systematical evaluation of the effects of sample collection procedures on low-molecular-weight serum/plasma proteome profiling. Proteomics 2006, 6, 3189–3198.
- Banks, RE; Stanley, AJ; Cairns, DA; Barrett, JH; Clarke, P; Thompson, D; Selby, PJ. Influences of blood sample processing on low-molecular-weight proteome identified by surface-enhanced laser desorption/ionization mass spectrometry. Clin. Chem 2005, 51, 1637–1649.
- West-Nielsen, M; Hogdall, EV; Marchiori, E; Hogdall, CK; Schou, C; Heegaard, NHH. Sample handling for mass spectrometric proteomic investigations of human sera. Anal. Chem 2005, 77, 5114–5123.
- Timms, JF; Arslan-Low, E; Gentry-Maharaj, A; Luo, Z; T’Jampens, D; Podust, VN; Ford, J; Fung, ET; Gammerman, A; Jacobs, I; Menon, U. Preanalytic influence of sample handling on SELDI-TOF serum protein profiles. Clin. Chem 2007, 53, 645–656.
- Rubin, O; Crettaz, D; Tissot, JD; Lion, N. Pre-analytical and methodological challenges in red blood cell microparticle proteomics. Talanta 2010, 82, 1–8.
- Olivieri, E; Herbert, B; Righetti, PG. The effect of protease inhibitors on the two-dimensional electrophoresis pattern of red blood cell membranes. Electrophoresis 2001, 22, 560–565.
- Davis, MT; Patterson, SD. Does the serum peptidome reveal hemostatic dysregulation? In Systems Biology: Applications and Perspectives; Bringmann, P, Butcher, EC, Parry, G, Weiss, B, Eds.; Springer: New York, NY, USA, 2007; Volume 61, pp. 23–44.
- Cox, AG; Peskin, AV; Paton, LN; Winterbourn, CC; Hampton, MB. Redox potential and peroxide reactivity of human peroxiredoxin 3. Biochemistry 2009, 48, 6495–6501.
- Le Moan, N; Tacnet, F; Toledano, MB. Protein-thiol oxidation, from single proteins to proteome-wide analyses. Methods Mol. Biol 2009, 476, 175–192.
- Traum, AZ; Wells, MP; Aivado, M; Libermann, TA; Ramoni, MF; Schachter, AD. SELDI-TOF MS of quadruplicate urine and serum samples to evaluate changes related to storage conditions. Proteomics 2006, 6, 1676–1680.
- Ulmert, D; Becker, C; Nilsson, JA; Piironen, T; Bjork, T; Hugosson, J; Berglund, G; Lilja, H. Reproducibility and accuracy of measurements of free and total prostate-specific antigen in serum vs plasma after long-term storage at-20 degrees C. Clin. Chem 2006, 52, 235–239.
- Insenser, M; Martínez-García, MÁ; Nieto, RM; San-Millán, JL; Escobar-Morreale, HF. Impact of the storage temperature on human plasma proteomic analysis: Implications for the use of human plasma collections in research. Proteomics Clin. Appl 2010, 4, 739–744.
- Wintrobe, MM; Buell, MV. Hyperproteinemia associated with multiple myeloma - With report of a case in which an extraordinary hyperproteinemia was associated with thrombosis of the retinal veins and symptoms suggesting Raynaud’s disease. Bull. Johns Hopkins Hosp 1933, 52, 156–165.
- Brouet, J-C; Clauvel, J-P; Danon, F; Klein, M; Seligmann, M. Biologic and clinical significance of cryoglobulins: A report of 86 cases. Am. J. Med 1974, 57, 775–788.
- Blain, H; Cacoub, P; Musset, L; Costedoat-Chalumeau, N; Silberstein, C; Chosidow, O; Godeau, P; Frances, C; Piette, JC. Cryofibrinogenaemia: A study of 49 patients. Clin. Exp. Immunol 2000, 120, 253–260.
- Robert, D; Barelli, S; Crettaz, D; Bart, PA; Schifferli, JA; Betticher, D; Tissot, JD. Clinical proteomics: Study of a cryogel. Proteomics 2006, 6, 3958–3960.
- Rai, AJ; Gelfand, CA; Haywood, BC; Warunek, DJ; Yi, J; Schuchard, MD; Mehigh, RJ; Cockrill, SL; Scott, GB; Tammen, H; Schulz-Knappe, P; Speicher, DW; Vitzthum, F; Haab, BB; Siest, G; Chan, DW. HUPO Plasma Proteome Project specimen collection and handling: towards the standardization of parameters for plasma proteome samples. Proteomics 2005, 5, 3262–3277.
|Table 1. Main pre-analytical conditions to respect for proteomic analyses of labile blood products and blood samples in biomarker discovery field.|
|Labile blood products||Blood sample for pathological-case biomarker research||References|
|Sampling||sample obtention||donor position||resting position|||
|tourniquet application||less than 30 seconds if possible, no more than 1 minute|||
|needle bore size||avoid too thin needle (21–23 gauge needles are preferred)||[70,71]|
|container type||material||approved plastic bags||vacuette®-like blood collection tubes||[72,73]|
|anticoagulant content||Citrate Phosphate Dextrose (CPD)||depends on analyses|||
|processing and handling||time between sampling and processing||up to 24 h at +4 °C or up to 4 h at +24 °C||[74,75]|
|transport time and temperature||controlled transport at +4 °C, must be as brief as possible|||
|centrifugation||prefer centrifugation at +4 °C, up to 3000 × g|||
|use of protease inhibitors||required to avoid proteolysis||[78,79]|
|use of antioxidant reagents||needed in case of redox proteomic studies||[80,81]|
|storage||temperature||original sample||+4 °C||+22 °C||−25 °C||−20/−30 °C or −80 °C||[73,82,83]|
|proteinic extracts||−20/−30 °C and −80 °C stored extracts are both suitable|||
|freeze/thaw cycles||better only once, but several cycles do not affect proteomic patterns||[73,82,83]|
|case of cryoproteins||always work at 37 °C (pre-heated containers)|||
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