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Production and Characterization of Fengycin by Indigenous Bacillus subtilis F29-3 Originating from a Potato Farm
Graduate School of Biotechnology and Bioengineering, Yuan Ze University, Chung-Li, Taoyuan 320, Taiwan
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Received: 8 October 2010; in revised form: 24 October 2010 / Accepted: 10 November 2010 / Published: 12 November 2010
Abstract: Fengycin, a lipopeptide biosurfactant, was produced by indigenous Bacillus subtilis F29-3 isolated from a potato farm. Although inhibiting the growth of filamentous fungi, the fengycin is ineffective against yeast and bacteria. In this study, fengycin was isolated from fermentation broth of B. subtilis F29-3 via acidic precipitation (pH 2.0 with 5 N HCl) followed by purification using ultrafiltration and nanofiltration. The purified fengycin product was characterized qualitatively by using fast atom bombardment-mass spectrometer, Fourier transform infrared spectrometer, ultraviolet-visible spectrophotometer, 13C-nuclear magnetic resonance spectrometer and matrix assisted laser desorption ionization-time of flight, followed by quantitative analysis using reversed-phase HPLC system. This study also attempted to increase fengycin production by B. subtilis F29-3 in order to optimize the fermentation medium constituents. The fermentation medium composition was optimized using response surface methodology (RSM) to increase fengycin production from B. subtilis F29-3. According to results of the five-level four-factor central composite design, the composition of soybean meal, NaNO3, MnSO4·4H2O, mannitol-mannitol, soybean meal-mannitol, soybean meal‑soybean meal, NaNO3-NaNO3 and MnSO4·4H2O-MnSO4·4H2O significantly affected production. The simulation model produced a coefficient of determination (R2) of 0.9043, capable of accounting for 90.43% variability of the data. Results of the steepest ascent and central composite design indicated that 26.2 g/L of mannitol, 21.9 g/L of soybean meal, 3.1 g/L of NaNO3 and 0.2 g/L of MnSO4·4H2O represented the optimal medium composition, leading to the highest production of fengycin. Furthermore, the optimization strategy increased the fengycin production from 1.2 g/L to 3.5 g/L.
Keywords: fengycin; lipopeptide biosurfactants; media optimization
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Wei, Y.-H.; Wang, L.-C.; Chen, W.-C.; Chen, S.-Y. Production and Characterization of Fengycin by Indigenous Bacillus subtilis F29-3 Originating from a Potato Farm. Int. J. Mol. Sci. 2010, 11, 4526-4538.
Wei Y-H, Wang L-C, Chen W-C, Chen S-Y. Production and Characterization of Fengycin by Indigenous Bacillus subtilis F29-3 Originating from a Potato Farm. International Journal of Molecular Sciences. 2010; 11(11):4526-4538.
Wei, Yu-Hong; Wang, Li-Chuan; Chen, Wei-Chuan; Chen, Shan-Yu. 2010. "Production and Characterization of Fengycin by Indigenous Bacillus subtilis F29-3 Originating from a Potato Farm." Int. J. Mol. Sci. 11, no. 11: 4526-4538.