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Int. J. Mol. Sci. 2010, 11(11), 4526-4538; doi:10.3390/ijms11114526
Article

Production and Characterization of Fengycin by Indigenous Bacillus subtilis F29-3 Originating from a Potato Farm

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Received: 8 October 2010; in revised form: 24 October 2010 / Accepted: 10 November 2010 / Published: 12 November 2010
(This article belongs to the Special Issue Biosurfactants)
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Abstract: Fengycin, a lipopeptide biosurfactant, was produced by indigenous Bacillus subtilis F29-3 isolated from a potato farm. Although inhibiting the growth of filamentous fungi, the fengycin is ineffective against yeast and bacteria. In this study, fengycin was isolated from fermentation broth of B. subtilis F29-3 via acidic precipitation (pH 2.0 with 5 N HCl) followed by purification using ultrafiltration and nanofiltration. The purified fengycin product was characterized qualitatively by using fast atom bombardment-mass spectrometer, Fourier transform infrared spectrometer, ultraviolet-visible spectrophotometer, 13C-nuclear magnetic resonance spectrometer and matrix assisted laser desorption ionization-time of flight, followed by quantitative analysis using reversed-phase HPLC system. This study also attempted to increase fengycin production by B. subtilis F29-3 in order to optimize the fermentation medium constituents. The fermentation medium composition was optimized using response surface methodology (RSM) to increase fengycin production from B. subtilis F29-3. According to results of the five-level four-factor central composite design, the composition of soybean meal, NaNO3, MnSO4·4H2O, mannitol-mannitol, soybean meal-mannitol, soybean meal‑soybean meal, NaNO3-NaNO3 and MnSO4·4H2O-MnSO4·4H2O significantly affected production. The simulation model produced a coefficient of determination (R2) of 0.9043, capable of accounting for 90.43% variability of the data. Results of the steepest ascent and central composite design indicated that 26.2 g/L of mannitol, 21.9 g/L of soybean meal, 3.1 g/L of NaNO3 and 0.2 g/L of MnSO4·4H2O represented the optimal medium composition, leading to the highest production of fengycin. Furthermore, the optimization strategy increased the fengycin production from 1.2 g/L to 3.5 g/L.
Keywords: fengycin; lipopeptide biosurfactants; media optimization fengycin; lipopeptide biosurfactants; media optimization
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Wei, Y.-H.; Wang, L.-C.; Chen, W.-C.; Chen, S.-Y. Production and Characterization of Fengycin by Indigenous Bacillus subtilis F29-3 Originating from a Potato Farm. Int. J. Mol. Sci. 2010, 11, 4526-4538.

AMA Style

Wei Y-H, Wang L-C, Chen W-C, Chen S-Y. Production and Characterization of Fengycin by Indigenous Bacillus subtilis F29-3 Originating from a Potato Farm. International Journal of Molecular Sciences. 2010; 11(11):4526-4538.

Chicago/Turabian Style

Wei, Yu-Hong; Wang, Li-Chuan; Chen, Wei-Chuan; Chen, Shan-Yu. 2010. "Production and Characterization of Fengycin by Indigenous Bacillus subtilis F29-3 Originating from a Potato Farm." Int. J. Mol. Sci. 11, no. 11: 4526-4538.



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