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Antioxidant Activities of Extract and Fractions from the Hypocotyls of the Mangrove Plant Kandelia candel
Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems, School of Life Sciences, Xiamen University, Xiamen 361005, China
* Author to whom correspondence should be addressed.
Received: 30 September 2010; in revised form: 17 October 2010 / Accepted: 19 October 2010 / Published: 21 October 2010
Abstract: The antioxidant activities of 70% acetone extract (70% AE) from the hypocotyls of the mangrove plant Kandelia candel and its fractions of petroleum ether (PF), ethyl acetate (EF), water (WF), and the LF (WF fraction further purified through a Sephadex LH‑20 column), were investigated by the 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging and ferric reducing/antioxidant power (FRAP) assays. The results showed that all the extract and fractions possessed potent antioxidant activity. There was a significant linear correlation between the total phenolics concentration and the ferric reducing power or free radical scavenging activity of the extract and fractions. Among the extract and fractions, the LF fraction exhibits the best antioxidant performance. The MALDT-TOF MS and HPLC analyses revealed that the phenolic compounds associated with the antioxidant activity of the LF fraction contains a large number of procyanidins and a small amount of prodelphinidins, and the epicatechin is the main extension unit.
Keywords: Kandelia candel; hypocotyl; antioxidant activity; MALDI-TOF MS; HPLC
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Wei, S.-D.; Zhou, H.-C.; Lin, Y.-M. Antioxidant Activities of Extract and Fractions from the Hypocotyls of the Mangrove Plant Kandelia candel. Int. J. Mol. Sci. 2010, 11, 4080-4093.
Wei S-D, Zhou H-C, Lin Y-M. Antioxidant Activities of Extract and Fractions from the Hypocotyls of the Mangrove Plant Kandelia candel. International Journal of Molecular Sciences. 2010; 11(10):4080-4093.
Wei, Shu-Dong; Zhou, Hai-Chao; Lin, Yi-Ming. 2010. "Antioxidant Activities of Extract and Fractions from the Hypocotyls of the Mangrove Plant Kandelia candel." Int. J. Mol. Sci. 11, no. 10: 4080-4093.