In Vitro Efficacy of Myxococcus fulvus ANSM068 to Biotransform Aflatoxin B₁

Abstract: Aflatoxin B₁ (AFB₁) is commonly found in cereals and animal feeds and causes a significant threat to the food industry and animal production. Several microbial isolates with high AFB₁ transformation ability have been identified in our previous studies. The aim of this research was to characterize one of those isolates, Myxococcus fulvus ANSM068, and to explore its biotransformation mechanism. The bacterial isolate of M. fulvus ANSM068, isolated from deer feces, was able to transform AFB₁ by 80.7% in liquid VY/2 medium after incubation at 30 °C for 72 h. The supernatant of the bacterial culture was more effective in transforming AFB₁ as compared to the cells alone and the cell extract. The transformation activity was significantly reduced and eradicated after the culture supernatant was treated with proteinase K, proteinase K plus SDS and heating. Culture conditions, including nitrogen source, initial pH and incubation temperature were evaluated for an optimal AFB₁ transformation. Liquid chromatography mass spectrometry (LCMS) analyses showed that AFB₁ was transformed to a structurally different compound. Infrared analysis (IR) indicated that the lactone ring on the AFB₁ molecule was modified by the culture supernatant. Chromatographies on DEAE-Ion exchange and Sephadex-Molecular sieve and SDS-PAGE electrophoresis were used to determine active components from the culture supernatant, indicating that enzyme(s) were responsible for the AFB₁ biotransformation. This is the first report on AFB₁ transformation by a strain of myxobacteria through enzymatic reaction(s).