Int. J. Mol. Sci. 2010, 11(10), 3965-3976; doi:10.3390/ijms11103965
Article

Distribution of Endogenous Farnesyl Pyrophosphate and Four Species of Lysophosphatidic Acid in Rodent Brain

1 The Department of Psychological and Brain Sciences, Indiana University Bloomington, IN 47405, USA 2 The Gill Center for Biomolecular Science, Bloomington IN, 47405, USA 3 The Kinsey Institute for Research in Sex, Gender and Reproduction, Bloomington IN 47405, USA These authors contributed equally to this work.
* Author to whom correspondence should be addressed.
Received: 24 August 2010; in revised form: 12 October 2010 / Accepted: 13 October 2010 / Published: 15 October 2010
(This article belongs to the Special Issue Biomarkers)
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Abstract: Lysophosphatidic acid (LPA) is the umbrella term for lipid signaling molecules that share structural homology and activate the family of LPA receptors. Farnesyl Pyrophosphate (FPP) is commonly known as an intermediate in the synthesis of steroid hormones; however, its function as a signaling lipid is beginning to be explored. FPP was recently shown to an activator of the G-protein coupled receptor 92 (also known as LPA5) of the calcium channel TRPV3. The LPA receptors (including GPR92) are associated with the signal transduction of noxious stimuli, however, very little is known about the distribution of their signaling ligands (LPAs and FPP) in the brain. Here, using HPLC/MS/MS, we developed extraction and analytical methods for measuring levels of FPP and 4 species of LPA (palmitoyl, stearoyl, oleoyl and arachidonoyl-sn-glycerol-3 phosphate) in rodent brain. Relative distributions of each of the five compounds was significantly different across the brain suggesting divergent functionality for each as signaling molecules based on where and how much of each is being produced. Brainstem, midbrain, and thalamus contained the highest levels measured for each compound, though none in the same ratios while relatively small amounts were produced in cortex and cerebellum. These data provide a framework for investigations into functional relationships of these lipid ligands in specific brain areas, many of which are associated with the perception of pain.
Keywords: LPA; FPP; GPR92; TRPV3, LC/MS/MS; pain

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MDPI and ACS Style

Lee, S.H.; Raboune, S.; Walker, J.M.; Bradshaw, H.B. Distribution of Endogenous Farnesyl Pyrophosphate and Four Species of Lysophosphatidic Acid in Rodent Brain. Int. J. Mol. Sci. 2010, 11, 3965-3976.

AMA Style

Lee SH, Raboune S, Walker JM, Bradshaw HB. Distribution of Endogenous Farnesyl Pyrophosphate and Four Species of Lysophosphatidic Acid in Rodent Brain. International Journal of Molecular Sciences. 2010; 11(10):3965-3976.

Chicago/Turabian Style

Lee, Sung Ha; Raboune, Siham; Walker, J. Michael; Bradshaw, Heather B. 2010. "Distribution of Endogenous Farnesyl Pyrophosphate and Four Species of Lysophosphatidic Acid in Rodent Brain." Int. J. Mol. Sci. 11, no. 10: 3965-3976.

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