Next Article in Journal
Analysis of Cooperativity by Isothermal Titration Calorimetry
Next Article in Special Issue
Molecular Toxicology of Substances Released from Resin–Based Dental Restorative Materials
Previous Article in Journal
RNA Relics and Origin of Life
Previous Article in Special Issue
The Evaluation of the Possibilities of Using PLGA Co-Polymer and Its Composites with Carbon Fibers or Hydroxyapatite in the Bone Tissue Regeneration Process – in Vitro and in Vivo Examinations
Article Menu

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2009, 10(8), 3442-3456; doi:10.3390/ijms10083442

The Role of Bloom Index of Gelatin on the Interaction with Retinal Pigment Epithelial Cells

1
Institute of Biochemical and Biomedical Engineering, Chang Gung University, Taoyuan 33302, Taiwan
2
Biomedical Engineering Research Center, Chang Gung University, Taoyuan 33302, Taiwan
3
Molecular Medicine Research Center, Chang Gung University, Taoyuan 33302, Taiwan
Received: 29 June 2009 / Revised: 29 July 2009 / Accepted: 31 July 2009 / Published: 3 August 2009
(This article belongs to the Special Issue Biocompatibility of Materials)
View Full-Text   |   Download PDF [355 KB, uploaded 19 June 2014]   |  

Abstract

Biocompatible materials are of considerable interest in the development of cell/drug delivery carriers for therapeutic applications. This paper investigates the effects of the Bloom index of gelatin on its interaction with retinal pigment epithelial (RPE) cells. Following two days of culture of ARPE-19 cells with gelatin samples G75-100, G175, and G300, the in vitro biocompatibility was determined by cell proliferation and viability assays, and glutamate uptake measurements, as well as cytokine expression analyses. The mitochondrial dehydrogenase activity in the G300 groups was significantly lower than that of G75-100 and G175 groups. The Live/Dead assays also showed that the gelatin samples G300 induced mild cytotoxicity. In comparison with the treatment of gelatins with low Bloom index, the exposure to high Bloom strength gelatins markedly reduced the glutamate uptake capacity of ARPE-19 cells. One possible explanation for these observations is that the presence of gelatin samples G300 with high viscosity in the medium may affect the nutrient availability to cultured cells. The analyses of pro-inflammatory cytokine IL-6 expression at both mRNA and protein levels showed that the gelatins with low Bloom index caused less cellular inflammatory reaction and had more acceptable biocompatibility than their high Bloom strength counterparts. These findings suggest that the Bloom index gives influence on cellular responses to gelatin materials. View Full-Text
Keywords: gelatin; Bloom index; in vitro biocompatibility; retinal pigment epithelial cells gelatin; Bloom index; in vitro biocompatibility; retinal pigment epithelial cells
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Lai, J.Y. The Role of Bloom Index of Gelatin on the Interaction with Retinal Pigment Epithelial Cells. Int. J. Mol. Sci. 2009, 10, 3442-3456.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top