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Int. J. Mol. Sci. 2009, 10(7), 2999-3011; doi:10.3390/ijms10072999
Article

Molecular Characterization of Tb, a New Approach for an Ancient Brucellaphage

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1 Department of Epidemiology, College of Preventive Medicine, Third Military Medical University / Gaotanyan road 30, Shapingba district, Chongqing 400038, China 2 State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Diseases Control and Prevention, China CDC / Liu zi No.5, Changping district, Beijing, 102206, China 3 Institute of Combine Injury, School of Preventive Medicine, State Key Laboratory of Trauma, Burns and Combined Injury, Third Military Medical University / Gaotanyan road 30, Shapingba district, Chongqing 400038, China
* Authors to whom correspondence should be addressed.
Received: 7 June 2009 / Accepted: 25 June 2009 / Published: 3 July 2009
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract

Tb (Tbilisi), the reference Brucellaphage strain, was classified as a member of the Podoviridae family with icosahedral capsids (57 ± 2 nm diameter) and short tails (32 ± 3 nm long). Brucellaphage DNA was double stranded and unmethylated; its molecular size was 34.5 kilobase pairs. Some sequences were found through RAPD analysis, TA cloning technology, and structural proteins were observed by using SDS-PAGE. Thus, the results have laid the foundation for the wider use of Brucellaphage’s basic mechanisms and practical applications.
Keywords: brucellaphage; molecular characterization; random amplified polymorphic DNA brucellaphage; molecular characterization; random amplified polymorphic DNA
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Zhu, C.-Z.; Xiong, H.-Y.; Han, J.; Cui, B.-Y.; Piao, D.-R.; Li, Y.-F.; Jiang, H.; Ren, Q.; Ma, X.-Y.; Chai, Y.-M.; Huang, X.; Zhao, H.-Y.; Li, L.-Y. Molecular Characterization of Tb, a New Approach for an Ancient Brucellaphage. Int. J. Mol. Sci. 2009, 10, 2999-3011.

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