Int. J. Mol. Sci. 2009, 10(7), 2999-3011; doi:10.3390/ijms10072999
Article

Molecular Characterization of Tb, a New Approach for an Ancient Brucellaphage

Received: 7 June 2009; Accepted: 25 June 2009 / Published: 3 July 2009
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Tb (Tbilisi), the reference Brucellaphage strain, was classified as a member of the Podoviridae family with icosahedral capsids (57 ± 2 nm diameter) and short tails (32 ± 3 nm long). Brucellaphage DNA was double stranded and unmethylated; its molecular size was 34.5 kilobase pairs. Some sequences were found through RAPD analysis, TA cloning technology, and structural proteins were observed by using SDS-PAGE. Thus, the results have laid the foundation for the wider use of Brucellaphage’s basic mechanisms and practical applications.
Keywords: brucellaphage; molecular characterization; random amplified polymorphic DNA
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MDPI and ACS Style

Zhu, C.-Z.; Xiong, H.-Y.; Han, J.; Cui, B.-Y.; Piao, D.-R.; Li, Y.-F.; Jiang, H.; Ren, Q.; Ma, X.-Y.; Chai, Y.-M.; Huang, X.; Zhao, H.-Y.; Li, L.-Y. Molecular Characterization of Tb, a New Approach for an Ancient Brucellaphage. Int. J. Mol. Sci. 2009, 10, 2999-3011.

AMA Style

Zhu C-Z, Xiong H-Y, Han J, Cui B-Y, Piao D-R, Li Y-F, Jiang H, Ren Q, Ma X-Y, Chai Y-M, Huang X, Zhao H-Y, Li L-Y. Molecular Characterization of Tb, a New Approach for an Ancient Brucellaphage. International Journal of Molecular Sciences. 2009; 10(7):2999-3011.

Chicago/Turabian Style

Zhu, Cai-Zhong; Xiong, Hong-Yan; Han, Jing; Cui, Bu-Yun; Piao, Dong-Ri; Li, Ya-Fei; Jiang, Hai; Ren, Qian; Ma, Xiang-Yu; Chai, Ya-Ming; Huang, Xia; Zhao, Hong-Yan; Li, Lan-Yu. 2009. "Molecular Characterization of Tb, a New Approach for an Ancient Brucellaphage." Int. J. Mol. Sci. 10, no. 7: 2999-3011.

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