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Molecular Characterization of Tb, a New Approach for an Ancient Brucellaphage
Department of Epidemiology, College of Preventive Medicine, Third Military Medical University / Gaotanyan road 30, Shapingba district, Chongqing 400038, China
State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Diseases Control and Prevention, China CDC / Liu zi No.5, Changping district, Beijing, 102206, China
Institute of Combine Injury, School of Preventive Medicine, State Key Laboratory of Trauma, Burns and Combined Injury, Third Military Medical University / Gaotanyan road 30, Shapingba district, Chongqing 400038, China
* Authors to whom correspondence should be addressed.
Received: 7 June 2009; Accepted: 25 June 2009 / Published: 3 July 2009
Abstract: Tb (Tbilisi), the reference Brucellaphage strain, was classified as a member of the Podoviridae family with icosahedral capsids (57 ± 2 nm diameter) and short tails (32 ± 3 nm long). Brucellaphage DNA was double stranded and unmethylated; its molecular size was 34.5 kilobase pairs. Some sequences were found through RAPD analysis, TA cloning technology, and structural proteins were observed by using SDS-PAGE. Thus, the results have laid the foundation for the wider use of Brucellaphage’s basic mechanisms and practical applications.
Keywords: brucellaphage; molecular characterization; random amplified polymorphic DNA
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Zhu, C.-Z.; Xiong, H.-Y.; Han, J.; Cui, B.-Y.; Piao, D.-R.; Li, Y.-F.; Jiang, H.; Ren, Q.; Ma, X.-Y.; Chai, Y.-M.; Huang, X.; Zhao, H.-Y.; Li, L.-Y. Molecular Characterization of Tb, a New Approach for an Ancient Brucellaphage. Int. J. Mol. Sci. 2009, 10, 2999-3011.
Zhu C-Z, Xiong H-Y, Han J, Cui B-Y, Piao D-R, Li Y-F, Jiang H, Ren Q, Ma X-Y, Chai Y-M, Huang X, Zhao H-Y, Li L-Y. Molecular Characterization of Tb, a New Approach for an Ancient Brucellaphage. International Journal of Molecular Sciences. 2009; 10(7):2999-3011.
Zhu, Cai-Zhong; Xiong, Hong-Yan; Han, Jing; Cui, Bu-Yun; Piao, Dong-Ri; Li, Ya-Fei; Jiang, Hai; Ren, Qian; Ma, Xiang-Yu; Chai, Ya-Ming; Huang, Xia; Zhao, Hong-Yan; Li, Lan-Yu. 2009. "Molecular Characterization of Tb, a New Approach for an Ancient Brucellaphage." Int. J. Mol. Sci. 10, no. 7: 2999-3011.