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Int. J. Mol. Sci. 2009, 10(5), 2010-2018; doi:10.3390/ijms10052010
Article

Soluble Tumor Necrosis Factor Receptor Mediates Cell Proliferation on Lipopolysaccharide-Stimulated Cultured Human Decidual Stromal Cells

1
, 1
 and 2,*
Received: 1 April 2009; in revised form: 20 April 2009 / Accepted: 27 April 2009 / Published: 4 May 2009
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract: The tumor necrosis factor-alpha (TNF-α) cytokine receptor system modulates apoptosis in many cell types, so we have investigated the role of sTNFR1 in bacterial lipopolysaccharide (LPS)-induced cell death in cultured human decidual stromal cells, hypothesizing that sTNFR1 might play a central role in this action. In this work we characterized in vitro decidual stromal cell viability with LPS treatment and LPS and sTNFR1 co-treatment. We found that LPS treatment induced decidual stromal cell death in a dose-dependent manner and that sTNFR1 blocked the effect of the LPS treatment. There was a significant proliferation among cells co-incubated with LPS at 10 μg/mL and sTNFR1 at 0.1 μg/mL compared with LPS and sTNFR1 at 0.01, 0.05, 0.2 and 0.5 μg/mL (p < 0.01). This study demonstrated that LPS led to decidual stromal cell death in vitro but sTNFR1 down-regulates the cell death due to LPS under the same conditions. Taken together, these results suggested that sTNFR1 could participate in a protective mechanism against endotoxin.
Keywords: Decidual stromal cell (DSC); soluble tumor necrosis factor receptor 1 (sTNFR1); membrane tumor necrosis factor receptor 1 (mTNFR1); pregnancy; lipopolysaccharide (LPS) Decidual stromal cell (DSC); soluble tumor necrosis factor receptor 1 (sTNFR1); membrane tumor necrosis factor receptor 1 (mTNFR1); pregnancy; lipopolysaccharide (LPS)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Yu, X.-W.; Zhang, X.-W.; Li, X. Soluble Tumor Necrosis Factor Receptor Mediates Cell Proliferation on Lipopolysaccharide-Stimulated Cultured Human Decidual Stromal Cells. Int. J. Mol. Sci. 2009, 10, 2010-2018.

AMA Style

Yu X-W, Zhang X-W, Li X. Soluble Tumor Necrosis Factor Receptor Mediates Cell Proliferation on Lipopolysaccharide-Stimulated Cultured Human Decidual Stromal Cells. International Journal of Molecular Sciences. 2009; 10(5):2010-2018.

Chicago/Turabian Style

Yu, Xue-Wen; Zhang, Xin-Wen; Li, Xu. 2009. "Soluble Tumor Necrosis Factor Receptor Mediates Cell Proliferation on Lipopolysaccharide-Stimulated Cultured Human Decidual Stromal Cells." Int. J. Mol. Sci. 10, no. 5: 2010-2018.


Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert