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Int. J. Mol. Sci. 2009, 10(5), 2010-2018; https://doi.org/10.3390/ijms10052010

Soluble Tumor Necrosis Factor Receptor Mediates Cell Proliferation on Lipopolysaccharide-Stimulated Cultured Human Decidual Stromal Cells

1
Center of Maternal and Child Health, First Affiliated Hospital of Medical School in Xi’an Jiaotong University, Xi’an, 710061, P. R. China
2
Center of Molecular Biological Medicine, First Affiliated Hospital of Medical School in Xi’an Jiaotong University, Xi’an, 710061, P. R. China
*
Author to whom correspondence should be addressed.
Received: 1 April 2009 / Revised: 20 April 2009 / Accepted: 27 April 2009 / Published: 4 May 2009
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
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Abstract

The tumor necrosis factor-alpha (TNF-α) cytokine receptor system modulates apoptosis in many cell types, so we have investigated the role of sTNFR1 in bacterial lipopolysaccharide (LPS)-induced cell death in cultured human decidual stromal cells, hypothesizing that sTNFR1 might play a central role in this action. In this work we characterized in vitro decidual stromal cell viability with LPS treatment and LPS and sTNFR1 co-treatment. We found that LPS treatment induced decidual stromal cell death in a dose-dependent manner and that sTNFR1 blocked the effect of the LPS treatment. There was a significant proliferation among cells co-incubated with LPS at 10 μg/mL and sTNFR1 at 0.1 μg/mL compared with LPS and sTNFR1 at 0.01, 0.05, 0.2 and 0.5 μg/mL (p < 0.01). This study demonstrated that LPS led to decidual stromal cell death in vitro but sTNFR1 down-regulates the cell death due to LPS under the same conditions. Taken together, these results suggested that sTNFR1 could participate in a protective mechanism against endotoxin. View Full-Text
Keywords: Decidual stromal cell (DSC); soluble tumor necrosis factor receptor 1 (sTNFR1); membrane tumor necrosis factor receptor 1 (mTNFR1); pregnancy; lipopolysaccharide (LPS) Decidual stromal cell (DSC); soluble tumor necrosis factor receptor 1 (sTNFR1); membrane tumor necrosis factor receptor 1 (mTNFR1); pregnancy; lipopolysaccharide (LPS)
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Yu, X.-W.; Zhang, X.-W.; Li, X. Soluble Tumor Necrosis Factor Receptor Mediates Cell Proliferation on Lipopolysaccharide-Stimulated Cultured Human Decidual Stromal Cells. Int. J. Mol. Sci. 2009, 10, 2010-2018.

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