Int. J. Mol. Sci. 2009, 10(12), 5217-5223; doi:10.3390/ijms10125217
Article

Enzymatic Reactions in Near Critical CO2: The Effect of Pressure on Phenol Removal by Tyrosinase

1email, 1email, 2email, 3email, 1email and 1,* email
Received: 14 August 2009; in revised form: 9 November 2009 / Accepted: 16 November 2009 / Published: 1 December 2009
(This article belongs to the Special Issue Supercritical Carbon Dioxide)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: The use of enzymes in supercritical CO2 (SCCO2) has received extensive attention in recent years. Biocatalysts have the advantage of substrate specificity and SCCO2 offers several advantages over liquid solvents. This work deals with the utilization of SCCO2 as a medium for the enzymatic removal of phenol from aqueous solutions using tyrosinase. Since the presence of oxygen is crucial for the enzyme-catalyzed oxidation, the substantial solvating power of SCCO2 makes it a promising medium for such reactions. The conversion of phenol was higher at 10 MPa. Under near critical conditions (7 MPa, 35 ºC), the addition of air at 5 × 105 Pa of pressure improved phenol removal.
Keywords: tyrosinase; supercritical CO2; enzymatic reaction; phenol
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MDPI and ACS Style

Amaral, P.; Garcia, D.; Cardoso, M.; Mendes, M.; Coelho, M.A.; Pessoa, F. Enzymatic Reactions in Near Critical CO2: The Effect of Pressure on Phenol Removal by Tyrosinase. Int. J. Mol. Sci. 2009, 10, 5217-5223.

AMA Style

Amaral P, Garcia D, Cardoso M, Mendes M, Coelho MA, Pessoa F. Enzymatic Reactions in Near Critical CO2: The Effect of Pressure on Phenol Removal by Tyrosinase. International Journal of Molecular Sciences. 2009; 10(12):5217-5223.

Chicago/Turabian Style

Amaral, Priscilla; Garcia, Daniela; Cardoso, Miguel; Mendes, Marisa; Coelho, Maria Alice; Pessoa, Fernando. 2009. "Enzymatic Reactions in Near Critical CO2: The Effect of Pressure on Phenol Removal by Tyrosinase." Int. J. Mol. Sci. 10, no. 12: 5217-5223.

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