Int. J. Mol. Sci. 2009, 10(1), 37-49; doi:10.3390/ijms10010037
Article

Developmental Toxicity of Ochratoxin A in Rat Embryo Midbrain Micromass Cultures

Received: 5 November 2008; in revised form: 15 December 2008 / Accepted: 18 December 2008 / Published: 27 December 2008
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Embryonic midbrain micromass cultures were exposed for five days to ochratoxin A (OTA) at seven concentrations (ranging from 0.16 to 10 ?g/mL). Cell viability was assessed in neutral red uptake test (NRU), and differentiation - by immunoenzymatic determination of structural proteins (?III-tubulin, MAP2, GFAP) expression level as well as by computer image analysis. Dose dependent decrease in cell number and differentiation was observed. Concentration-response curves were analysed and the mean inhibition concentrations (?g/mL) for cytotoxicity (IC50) and differentiation (ID50) were calculated. There were no significant differences in the sensitivity of neurons in early and late stage of differentiation and astrocytes to the toxic activity of this compound. For all endpoints ID50 value was very low (< 10 ?g/mL) so OTA was classified as a strong teratogen. IC50/ ID50 ratios <2 pointed out that with harmful action of OTA the basic cytotoxicity should be connected.
Keywords: Ochratoxin A; developmental neurotoxicity; in vitro micromass cultures; embryonic midbrain cells; computer image analysis; immunocytochemistry
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MDPI and ACS Style

Wilk–Zasadna, I.; Minta, M. Developmental Toxicity of Ochratoxin A in Rat Embryo Midbrain Micromass Cultures. Int. J. Mol. Sci. 2009, 10, 37-49.

AMA Style

Wilk–Zasadna I, Minta M. Developmental Toxicity of Ochratoxin A in Rat Embryo Midbrain Micromass Cultures. International Journal of Molecular Sciences. 2009; 10(1):37-49.

Chicago/Turabian Style

Wilk–Zasadna, Iwona; Minta, Maria. 2009. "Developmental Toxicity of Ochratoxin A in Rat Embryo Midbrain Micromass Cultures." Int. J. Mol. Sci. 10, no. 1: 37-49.

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