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Developmental Toxicity of Ochratoxin A in Rat Embryo Midbrain Micromass Cultures
Department of Pharmacology & Toxicology, National Veterinary Research Institute, Al. Partyzantow 57, 24-100 Pulawy, Poland
* Author to whom correspondence should be addressed.
Received: 5 November 2008; in revised form: 15 December 2008 / Accepted: 18 December 2008 / Published: 27 December 2008
Abstract: Embryonic midbrain micromass cultures were exposed for five days to ochratoxin A (OTA) at seven concentrations (ranging from 0.16 to 10 ?g/mL). Cell viability was assessed in neutral red uptake test (NRU), and differentiation - by immunoenzymatic determination of structural proteins (?III-tubulin, MAP2, GFAP) expression level as well as by computer image analysis. Dose dependent decrease in cell number and differentiation was observed. Concentration-response curves were analysed and the mean inhibition concentrations (?g/mL) for cytotoxicity (IC50) and differentiation (ID50) were calculated. There were no significant differences in the sensitivity of neurons in early and late stage of differentiation and astrocytes to the toxic activity of this compound. For all endpoints ID50 value was very low (< 10 ?g/mL) so OTA was classified as a strong teratogen. IC50/ ID50 ratios <2 pointed out that with harmful action of OTA the basic cytotoxicity should be connected.
Keywords: Ochratoxin A; developmental neurotoxicity; in vitro micromass cultures; embryonic midbrain cells; computer image analysis; immunocytochemistry
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Wilk–Zasadna, I.; Minta, M. Developmental Toxicity of Ochratoxin A in Rat Embryo Midbrain Micromass Cultures. Int. J. Mol. Sci. 2009, 10, 37-49.
Wilk–Zasadna I, Minta M. Developmental Toxicity of Ochratoxin A in Rat Embryo Midbrain Micromass Cultures. International Journal of Molecular Sciences. 2009; 10(1):37-49.
Wilk–Zasadna, Iwona; Minta, Maria. 2009. "Developmental Toxicity of Ochratoxin A in Rat Embryo Midbrain Micromass Cultures." Int. J. Mol. Sci. 10, no. 1: 37-49.