Next Article in Journal
Evaluation of Sphingolipids in Wistar Rats Treated to Prolonged and Single Oral Doses of Fumonisin B1
Next Article in Special Issue
An Overview of Conventional and Emerging Analytical Methods for the Determination of Mycotoxins
Previous Article in Journal
Over-expression, Rapid Preparation and Some Properties of C-terminal BARc Region in PICK1
Previous Article in Special Issue
Impact of Deoxynivalenol on the Intestinal Microflora of Pigs
Int. J. Mol. Sci. 2009, 10(1), 37-49; doi:10.3390/ijms10010037
Article

Developmental Toxicity of Ochratoxin A in Rat Embryo Midbrain Micromass Cultures

 and *
Received: 5 November 2008; in revised form: 15 December 2008 / Accepted: 18 December 2008 / Published: 27 December 2008
View Full-Text   |   Download PDF [333 KB, uploaded 19 June 2014]   |   Browse Figures
Abstract: Embryonic midbrain micromass cultures were exposed for five days to ochratoxin A (OTA) at seven concentrations (ranging from 0.16 to 10 ?g/mL). Cell viability was assessed in neutral red uptake test (NRU), and differentiation - by immunoenzymatic determination of structural proteins (?III-tubulin, MAP2, GFAP) expression level as well as by computer image analysis. Dose dependent decrease in cell number and differentiation was observed. Concentration-response curves were analysed and the mean inhibition concentrations (?g/mL) for cytotoxicity (IC50) and differentiation (ID50) were calculated. There were no significant differences in the sensitivity of neurons in early and late stage of differentiation and astrocytes to the toxic activity of this compound. For all endpoints ID50 value was very low (< 10 ?g/mL) so OTA was classified as a strong teratogen. IC50/ ID50 ratios <2 pointed out that with harmful action of OTA the basic cytotoxicity should be connected.
Keywords: Ochratoxin A; developmental neurotoxicity; in vitro micromass cultures; embryonic midbrain cells; computer image analysis; immunocytochemistry Ochratoxin A; developmental neurotoxicity; in vitro micromass cultures; embryonic midbrain cells; computer image analysis; immunocytochemistry
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Export to BibTeX |
EndNote


MDPI and ACS Style

Wilk–Zasadna, I.; Minta, M. Developmental Toxicity of Ochratoxin A in Rat Embryo Midbrain Micromass Cultures. Int. J. Mol. Sci. 2009, 10, 37-49.

AMA Style

Wilk–Zasadna I, Minta M. Developmental Toxicity of Ochratoxin A in Rat Embryo Midbrain Micromass Cultures. International Journal of Molecular Sciences. 2009; 10(1):37-49.

Chicago/Turabian Style

Wilk–Zasadna, Iwona; Minta, Maria. 2009. "Developmental Toxicity of Ochratoxin A in Rat Embryo Midbrain Micromass Cultures." Int. J. Mol. Sci. 10, no. 1: 37-49.


Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert