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Molecules 2001, 6(2), 92-103; doi:10.3390/60100092
Article

Supercritical Fluid Extraction (SFE) of Monoterpenes from the Leaves of Melaleuca alternifolia (Tea Tree)

* , ,  and
Centre for Biostructural and Biomolecular Research, Building K12, University of Western Sydney, Hawkesbury Campus, Locked Bag 1797, Penrith South, NSW 1797, Australia
* Author to whom correspondence should be addressed.
Received: 23 November 2000 / Accepted: 30 November 2000 / Published: 16 January 2001
(This article belongs to the Special Issue RACI Natural Products Group Symposium)
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Abstract

The technique of supercritical fluid extraction (SFE) was applied to various sample matrices under a range of supercritical carbon dioxide (scCO2) densities and chamber temperatures. The purpose was to develop an effective extraction condition for the removal of eight target monoterpenes from Australian tea tree (Melaleuca alternifolia Cheel) leaves. The optimum conditions for extraction were found to be 0.25 g/mL scCO2 density at a chamber temperature of 110oC. These condition were most effective when applied to whole fresh and rehydrated whole dried leaves, where it yielded maximum recovery of target analytes with minimum change in oil composition for the extractor system employed. This study demonstrates the importance of the type of sample matrix used in SFE work, and that a different extraction protocol would need to be developed for each matrix.
Keywords: Supercritical Fluid Extraction; SFE; Melaleuca alternifolia; Australian Tea Tree Supercritical Fluid Extraction; SFE; Melaleuca alternifolia; Australian Tea Tree
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Wong, V.; Wyllie, S.G.; Cornwell, C.P.; Tronson, D. Supercritical Fluid Extraction (SFE) of Monoterpenes from the Leaves of Melaleuca alternifolia (Tea Tree). Molecules 2001, 6, 92-103.

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