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Molecules 2000, 5(12), 1259-1264; doi:10.3390/51201259

An Efficient Synthetic Strategy for the Preparation of Nucleic Acid-Encoded Peptide and Protein Libraries for In Vitro Evolution Protocols

Phylos, Inc., 128 Spring Street, Lexington, MA 02421, USA
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Received: 6 November 2000 / Accepted: 6 November 2000 / Published: 18 December 2000
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Abstract

We describe an improved synthetic strategy for the preparation of nucleic acid encoded peptide and protein libraries. A solid-phase format was used to prepare and purify a novel type of mRNA-template for in vitro mRNA-protein fusion synthesis. The present protocol simplifies and accelerates the preparation of fusion libraries and should prove most useful for in vitro protein evolution procedures which involve repetitive cycles of fusion library preparation and selection.
Keywords: Nucleic acid-encoded libraries; mRNA-protein fusion; PROfusionTM; in vitro selection; puromycin; psoralen Nucleic acid-encoded libraries; mRNA-protein fusion; PROfusionTM; in vitro selection; puromycin; psoralen
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Kurz, M.; Kuang, G.; Lohse, P.A. An Efficient Synthetic Strategy for the Preparation of Nucleic Acid-Encoded Peptide and Protein Libraries for In Vitro Evolution Protocols. Molecules 2000, 5, 1259-1264.

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