Molecules 2000, 5(12), 1259-1264; doi:10.3390/51201259
Article

An Efficient Synthetic Strategy for the Preparation of Nucleic Acid-Encoded Peptide and Protein Libraries for In Vitro Evolution Protocols

Received: 6 November 2000; Accepted: 6 November 2000 / Published: 18 December 2000
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: We describe an improved synthetic strategy for the preparation of nucleic acid encoded peptide and protein libraries. A solid-phase format was used to prepare and purify a novel type of mRNA-template for in vitro mRNA-protein fusion synthesis. The present protocol simplifies and accelerates the preparation of fusion libraries and should prove most useful for in vitro protein evolution procedures which involve repetitive cycles of fusion library preparation and selection.
Keywords: Nucleic acid-encoded libraries; mRNA-protein fusion; PROfusionTM; in vitro selection; puromycin; psoralen
PDF Full-text Download PDF Full-Text [79 KB, uploaded 18 June 2014 19:25 CEST]

Export to BibTeX |
EndNote


MDPI and ACS Style

Kurz, M.; Kuang, G.; Lohse, P.A. An Efficient Synthetic Strategy for the Preparation of Nucleic Acid-Encoded Peptide and Protein Libraries for In Vitro Evolution Protocols. Molecules 2000, 5, 1259-1264.

AMA Style

Kurz M, Kuang G, Lohse PA. An Efficient Synthetic Strategy for the Preparation of Nucleic Acid-Encoded Peptide and Protein Libraries for In Vitro Evolution Protocols. Molecules. 2000; 5(12):1259-1264.

Chicago/Turabian Style

Kurz, Markus; Kuang, Guannan; Lohse, Peter A. 2000. "An Efficient Synthetic Strategy for the Preparation of Nucleic Acid-Encoded Peptide and Protein Libraries for In Vitro Evolution Protocols." Molecules 5, no. 12: 1259-1264.

Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert