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Molecules 2018, 23(9), 2258; https://doi.org/10.3390/molecules23092258

Separation and Analysis of Aspirin and Metformin HCl Using Green Subcritical Water Chromatography

Department of Chemistry, East Carolina University, Greenville, NC 27858, USA
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Received: 9 August 2018 / Revised: 31 August 2018 / Accepted: 3 September 2018 / Published: 5 September 2018
(This article belongs to the Section Analytical Chemistry)
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Abstract

Organic solvents are widely used in pharmaceutical and chemical industry for chromatographic separations. In recent years, subcritical water chromatography (SBWC) has shown ability in replacing hazardous organic solvents used in traditional high-performance liquid chromatography (HPLC). In this work, a pain killer—aspirin—and an antidiabetic drug—metformin HCl—were successfully separated on an XBridge C18 column using no organic solvents in the subcritical water chromatography mobile phase. Both traditional HPLC and subcritical water chromatography were used for comparison purposes. SBWC separation of metformin HCl and aspirin were achieved at 95 °C and 125 °C, respectively. The recovery for both active pharmaceutical ingredients (APIs) obtained by SBWC is 99% in comparing with the stated content of each drug. The relative standard deviation is less than 1% for SBWC assays developed in this work. This level of accuracy and precision achieved by SBWC is the same as that resulted by the traditional HPLC analysis. View Full-Text
Keywords: aspirin; metformin HCl; high performance liquid chromatography; HPLC; subcritical water chromatography; SBWC aspirin; metformin HCl; high performance liquid chromatography; HPLC; subcritical water chromatography; SBWC
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Doctor, N.; Yang, Y. Separation and Analysis of Aspirin and Metformin HCl Using Green Subcritical Water Chromatography. Molecules 2018, 23, 2258.

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