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Molecules 2018, 23(9), 2211; https://doi.org/10.3390/molecules23092211

Residue Depletion of Florfenicol and Florfenicol Amine in Broiler Chicken Claws and a Comparison of Their Concentrations in Edible Tissues Using LC–MS/MS

1
Department of Preventive Medicine, Faculty of Veterinary and Animal Sciences, University of Chile, Av. Santa Rosa, 11735 La Pintana, Santiago, Chile
2
Laboratory of Veterinary Pharmacology, Faculty of Veterinary and Animal Sciences, University of Chile, Av. Santa Rosa, 11735 La Pintana, Santiago, Chile
*
Author to whom correspondence should be addressed.
Academic Editors: Patricia Regal and Carlos M. Franco
Received: 19 July 2018 / Revised: 23 August 2018 / Accepted: 26 August 2018 / Published: 31 August 2018
(This article belongs to the Special Issue Trends in Veterinary Drug Analysis: Multiresidue and Omic Approaches)
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Abstract

Antimicrobial residues might persist in products and by-products destined for human or animal consumption. Studies exploring the depletion behavior of florfenicol residues in broiler chicken claws are scarce, even though claws can enter the food chain directly or indirectly. Hence, this study intended to assess the concentrations of florfenicol (FF) and florfenicol amine (FFA)—its active metabolite—in chicken claws from birds that were treated with a therapeutic dose of florfenicol. Furthermore, concentrations of these analytes in this matrix were compared with their concentrations in edible tissues at each sampling point. A group of 70 broiler chickens were raised under controlled conditions and used to assess residue depletion. Sampling points were on days 5, 10, 20, 25, 30, 35, and 40 after ceasing treatment, thus extending beyond the withdrawal period established for muscle tissue (30 days). Analytes were extracted using HPLC-grade water and acetone, and dichloromethane was used for the clean-up stage. Liquid chromatography coupled to mass spectroscopy detection (LC–MS/MS) was used to detect and quantify the analytes. The analytical methodology developed in this study was validated in-house and based on the recommendations described in the Commission Decision 2002/657/EC from the European Union. Analyte concentrations were calculated by linear regression analysis of calibration curves that were fortified using an internal standard of chloramphenicol-d5 (CAF-d5). The depletion time of FF and FFA was set at 74 days in claws, based on a 95% confidence level and using the limit of detection (LOD) as the cut-off point. Our findings show that FF and FFA can be found in chicken claws at higher concentrations than in muscle and liver samples at each sampling point. View Full-Text
Keywords: florfenicol; florfenicol amine; antimicrobial residues; muscle; liver; chicken claws; LC–MS/MS florfenicol; florfenicol amine; antimicrobial residues; muscle; liver; chicken claws; LC–MS/MS
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Pokrant, E.; Riquelme, R.; Maddaleno, A.; San Martín, B.; Cornejo, J. Residue Depletion of Florfenicol and Florfenicol Amine in Broiler Chicken Claws and a Comparison of Their Concentrations in Edible Tissues Using LC–MS/MS. Molecules 2018, 23, 2211.

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