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Molecules 2018, 23(9), 2195; https://doi.org/10.3390/molecules23092195

Organ-Specific Oxidative Events under Restrictive Versus Full Reperfusion Following Hemorrhagic Traumatic Shock in Rats

1
Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, AUVA Research Center for Traumatology, 1200 Vienna, Austria
2
Thermo Fisher Scientific, BRAHMS Biomarkers, Research Department, 16761 Hennigsdorf, Germany
*
Author to whom correspondence should be addressed.
Received: 25 July 2018 / Revised: 17 August 2018 / Accepted: 28 August 2018 / Published: 30 August 2018
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Abstract

Background aim: Reperfusion after hemorrhagic traumatic shock (HTS) is often associated with complications that are partly ascribed to the formation of reactive oxygen species (ROS). The aim of our study was to compare the effects of restrictive reperfusion (RR) to rapid full reperfusion (FR) on ROS formation and/or oxidative events. Materials and methods: Anesthetized male rats were randomly subjected to HTS followed by FR (75 mL/kg/h) or RR (30 mL/kg/h for 40 min, followed by 75 mL/kg/h) with Ringer’s solution (n = 8/group). Compartment-specific ROS formation was determined by infusion of ROS scavenger 1-hydroxy-3-carboxy-2,2,5,5-tetramethyl-pyrrolidine hydrochloride (CP-H) during resuscitation, followed by electron paramagnetic resonance spectroscopy. Sham-operated animals (n = 8) served as controls. The experiment was terminated 100 min post-shock. Results: Mean arterial pressure was significantly higher in the FR compared to the RR group during early reperfusion. Only RR animals, not FR animals, showed significantly higher ROS concentrations in erythrocytes (1951 ± 420 vs. 724 ± 75 AU) and in liver (474 ± 57 vs. 261 ± 21 AU) compared to sham controls. This was accompanied by elevated alanine aminotransferase and creatinine levels in RR animals compared to both shams and FR animals, while lipid peroxidation products (thiobarbituric acid reactive substances) were significantly increased only in the kidney in the FR group (p < 0.05). RR animals showed significantly higher plasma peroxiredoxin-4 values when compared to the FR group (20 ± 2 vs. 14 ± 0.5 RLU). Conclusion: Restrictive reperfusion after HTS is associated with increased ROS formation in erythrocytes and liver compared to sham controls. Moreover, the restrictive reperfusion is associated with a more pronounced injury to the liver and kidney, which is likely mediated by other than lipid peroxidation process and/or oxidative stress reactions. View Full-Text
Keywords: reactive oxygen species; peroxidation; ischemia; reperfusion injury; organ failure reactive oxygen species; peroxidation; ischemia; reperfusion injury; organ failure
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Penzenstadler, C.; Zifko, A.; Jafarmadar, M.; Schulte, J.; Struck, J.; Stainer, M.; Kozlov, A.; Bahrami, S. Organ-Specific Oxidative Events under Restrictive Versus Full Reperfusion Following Hemorrhagic Traumatic Shock in Rats. Molecules 2018, 23, 2195.

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