Next Article in Journal
The Substituent Effect on the Radical Scavenging Activity of Apigenin
Previous Article in Journal
Ethanolic Extract of Origanum vulgare Suppresses Propionibacterium acnes-Induced Inflammatory Responses in Human Monocyte and Mouse Ear Edema Models
Article Menu

Export Article

Open AccessArticle
Molecules 2018, 23(8), 1988; https://doi.org/10.3390/molecules23081988

In Vivo and In Vitro Anti-Arthritic Effects of Cardenolide-Rich and Caffeoylquinic Acid-Rich Fractions of Periploca forrestii

1
State Key Laboratory of Functions and Applications of Medicinal Plants, Key Laboratory of Pharmaceutics of Guizhou Province, Guizhou Medical University, Guiyang 550004, China
2
Engineering Research Center for the Development and Applications of Ethnic Medicines and TCM (Ministry of Education), Guizhou Medical University, Guiyang 550004, China
3
School of Pharmacy, Guizhou Medical University, Guiyang 550004, China
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Received: 9 July 2018 / Revised: 5 August 2018 / Accepted: 8 August 2018 / Published: 9 August 2018
(This article belongs to the Section Natural Products Chemistry)
View Full-Text   |   Download PDF [6983 KB, uploaded 9 August 2018]   |  

Abstract

Periploca forrestii Schltr. (P. forrestii) is a species used in Traditional Chinese Medicine (TCM) known as “Miao medicine”, and has a long history of use in the treatment of rheumatism, rheumatoid arthritis (RA), and joint pain. The present study aimed to evaluate the anti-arthritis effects of the cardenolide-rich and caffeoylquinic acid-rich fractions (CDLFs and CQAFs) of P. forrestii in collagen-induced arthritic (CIA) rats, and defined the mechanisms of therapeutic action in MH7A cells treated with TNF-α. Serum rheumatoid factor (RF), TNF-α, IL-6, IL-1β, PGE2, NO, SOD, and MDA were determined by ELISA or other commercially assay kits. Histopathological changes in ankle joint tissues were examined. The mRNA expressions of IL-1β, IL-6, COX-2, and iNOS in MH7A cells were measured by qRT-PCR assays. In addition, the expressions of iNOS, COX-2, and p65 proteins, and the phosphorylation of IκBα, p38, ERK1/2, and JNK proteins in MH7A cells were analyzed by Western blot. The results showed that CDLF and CQAF could suppress the paw swelling in CIA rats at different doses (125 mg/kg, 250 mg/kg, and 500 mg/kg). Histopathological examination suggests that the CDLF and CQAF significantly relieved the damage of the structure of the ankle joint in CIA rats. In addition, serum RF, TNF-α, IL-6, IL-1β, PGE2, NO, and MDA were decreased, along with increased activity of serum SOD. Furthermore, CDLF and CQAF downregulated the expressions of IL-1β, IL-6, COX-2, iNOS, and p65, and inhibited the phosphorylation of IκBα, p38, ERK1/2, and JNK in MH7A cells treated with TNF-α. These findings demonstrated that both CDLF and CQAF exhibited anti-arthritic activity, which might be associated with their inhibitory effects on the NF-κB and MAPK signaling pathways. View Full-Text
Keywords: rheumatoid arthritis; Periploca forrestii; cardenolide-rich fraction; caffeoylquinic acid-rich fraction; mitogen-activated protein kinase; nuclear factor κB rheumatoid arthritis; Periploca forrestii; cardenolide-rich fraction; caffeoylquinic acid-rich fraction; mitogen-activated protein kinase; nuclear factor κB
Figures

Graphical abstract

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

SciFeed

Share & Cite This Article

MDPI and ACS Style

Liu, T.; Wang, X.; He, Y.-L.; Wang, Y.; Dong, L.; Ma, X.; Zheng, L.; Liu, C.-H.; Wang, G.-C.; Zheng, J.; Lan, Y.-Y.; Li, Y.-J. In Vivo and In Vitro Anti-Arthritic Effects of Cardenolide-Rich and Caffeoylquinic Acid-Rich Fractions of Periploca forrestii. Molecules 2018, 23, 1988.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top