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Molecules 2018, 23(8), 1854; https://doi.org/10.3390/molecules23081854

Supercritical CO2 Extraction of Nannochloropsis sp.: A Lipidomic Study on the Influence of Pretreatment on Yield and Composition

Flemish Institute for Technological Research (VITO), Boeretang 200, 2400 Mol, Belgium
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Academic Editor: Yu Yang
Received: 11 June 2018 / Revised: 10 July 2018 / Accepted: 17 July 2018 / Published: 25 July 2018
(This article belongs to the Special Issue Sub- and Supercritical Fluids and Green Chemistry)
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Abstract

Algal lipids have gained wide interest in various applications ranging from biofuels to nutraceuticals. Given their complex nature composed of different lipid classes, a deep knowledge between extraction conditions and lipid characteristics is essential. In this paper, we investigated the influence of different pretreatments on lipid extraction with supercritical CO2 by a lipidomic approach. Pretreatment was found to double the total extraction yield, thereby reaching 23.1 wt.% comparable to the 26.9 wt.% obtained with chloroform/methanol. An increase in acylglycerides was concurrently observed, together with a nearly doubling of free fatty acids indicative of partial hydrolysis. Moreover, an alteration in the distribution of glyco- and phospholipids was noted, especially promoting digalactosyldiglycerides and phosphatidylcholine as compared to monogalactosyldiglycerides and phosphatidylglycerol. At optimized conditions, supercritical CO2 extraction provided a lipid extract richer in neutral lipids and poorer in phospholipids as compared to chloroform/methanol, though with a very similar fatty acid distribution within each lipid class. View Full-Text
Keywords: Nannochloropsis sp.; supercritical CO2 extraction; microalgae; pretreatment; lipidomic; UHPLC-PDA-HR-MS; glycolipids; phospholipids; glycerides; mass transfer limitations Nannochloropsis sp.; supercritical CO2 extraction; microalgae; pretreatment; lipidomic; UHPLC-PDA-HR-MS; glycolipids; phospholipids; glycerides; mass transfer limitations
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Elst, K.; Maesen, M.; Jacobs, G.; Bastiaens, L.; Voorspoels, S.; Servaes, K. Supercritical CO2 Extraction of Nannochloropsis sp.: A Lipidomic Study on the Influence of Pretreatment on Yield and Composition. Molecules 2018, 23, 1854.

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