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Molecules 2018, 23(7), 1829; https://doi.org/10.3390/molecules23071829

Decomposing the Mechanism of Qishen Granules in the Treatment of Heart Failure by a Quantitative Pathway Analysis Method

1,†
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2,†
,
1,†
,
1
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1
,
3,4
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1,* , 2,* and 1,*
1
School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China
2
School of Life Sciences and Technology, Tongji University, Shanghai 200092, China
3
Key Laboratory of Computational Biology, CAS-MPG Partner Institute for Computational Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China
4
School of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China
These authors contributed equally to this study.
*
Authors to whom correspondence should be addressed.
Received: 24 June 2018 / Revised: 17 July 2018 / Accepted: 18 July 2018 / Published: 23 July 2018
(This article belongs to the Special Issue Natural Product Pharmacology and Medicinal Chemistry)
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Abstract

Qishen granules (QSG) have beneficial therapeutic effects for heart failure, but the effects of decomposed recipes, including Wenyang Yiqi Huoxue (WYH) and Qingre Jiedu (QJ), are not clear. In this study, the efficacy of WYH and QJ on heart failure is evaluated by using transverse aortic constriction (TAC) induced mice and the significantly changed genes in heart tissues were screened with a DNA array. Furthermore, a new quantitative pathway analysis tool is developed to evaluate the differences of pathways in different groups and to identify the pharmacological contributions of the decomposed recipes. Finally, the related genes in the significantly changed pathways are verified by a real-time polymerase chain reaction and a Western blot. Our data show that both QJ and WYH improve the left ventricular ejection fraction, which explain their contributions to protect against heart failure. In the energy metabolism, QJ achieves the therapeutic effects of QSG through nicotinamide nucleotide transhydrogenase (Nnt)-mediated mechanisms. In ventricular remodeling and inflammation reactions, QJ and WYH undertake the therapeutic effects through 5′-nucleotidase ecto (Nt5e)-mediated mechanisms. Together, QJ and WYH constitute the therapeutic effects of QSG and play important roles in myocardial energy metabolism and inflammation, which can exert therapeutic effects for heart failure. View Full-Text
Keywords: Qishen granules; heart failure; quantitative pathway analysis; decomposing formula; Wenyang Yiqi Huoxue; Qingre Jiedu Qishen granules; heart failure; quantitative pathway analysis; decomposing formula; Wenyang Yiqi Huoxue; Qingre Jiedu
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Ren, W.; Gao, S.; Zhang, H.; Ren, Y.; Yu, X.; Lin, W.; Guo, S.; Zhu, R.; Wang, W. Decomposing the Mechanism of Qishen Granules in the Treatment of Heart Failure by a Quantitative Pathway Analysis Method. Molecules 2018, 23, 1829.

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