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Molecules 2018, 23(7), 1826; https://doi.org/10.3390/molecules23071826

Assessing the Antioxidant Properties of Larrea tridentata Extract as a Potential Molecular Therapy against Oxidative Stress

1
Department of Biology, University of Massachusetts, Amherst, MA 01003-9297, USA
2
Department of Chemistry and Biochemistry, Border Biomedical Research Center, The University of Texas at El Paso, El Paso, TX 79968, USA
3
Department of Biology, Border Biomedical Research Center, The University of Texas at El Paso, El Paso, TX 79968, USA
*
Authors to whom correspondence should be addressed.
Received: 19 June 2018 / Revised: 13 July 2018 / Accepted: 18 July 2018 / Published: 23 July 2018
(This article belongs to the Special Issue Directed Drug Design and Molecular Therapy)
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Abstract

Oxidative stress has been linked to neurodegenerative diseases such as Huntington’s, Parkinson’s, Alzheimer’s and amyotrophic lateral sclerosis diseases. Larrea tridentata (LT) also known as Creosote Bush is an evergreen shrub found in the Chihuahuan desert which has been used medicinally by Native American tribes in southwestern North America and the Amerindians of South America. However, studies of the antioxidant capacity of the crude extract of LT towards the discovery of novel molecular therapies bearing antioxidants and drug-like properties are lacking. In this study, we assessed the antioxidant properties of Larrea tridentata, collected specifically from the Chihuahuan desert in the region of El Paso del Norte, TX, USA. LT phytochemicals were obtained from three different extracts (ethanol; ethanol: water (60:40) and water). Then the extracts were evaluated in eight different assays (DPPH, ABTS, superoxide; FRAP activity, nitric oxide, phenolic content, UV visible absorption and cytotoxicity in non-cancerous HS27 cells). The three extracts were not affecting the HS27 cells at concentrations up to 120 µg/mL. Among the three extracts, we found that the mixture of ethanol: water (60:40) LT extract has the most efficient antioxidant properties (IC50 (DPPH at 30 min) = 111.7 ± 3.8 μg/mL; IC50 (ABTS) = 8.49 ± 2.28 μg/mL; IC50 (superoxide) = 0.43 ± 0.17 μg/mL; IC50 (NO) = 230.4 ± 130.4 μg/mL; and the highest phenolic content was estimated to 212.46 ± 7.05 mg GAE/L). In addition, there was a strong correlation between phenolic content and the free-radical scavenging activity assays. HPLC-MS study identified nine compounds from the LT-ethanol: water extract including Justicidin B and Beta peltain have been previously reported as secondary metabolites of Larrea tridentata. View Full-Text
Keywords: oxidative stress; Larrea tridentata extract; antioxidant in vitro assays; cytotoxicity assay oxidative stress; Larrea tridentata extract; antioxidant in vitro assays; cytotoxicity assay
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Skouta, R.; Morán-Santibañez, K.; Valenzuela, C.A.; Vasquez, A.H.; Fenelon, K. Assessing the Antioxidant Properties of Larrea tridentata Extract as a Potential Molecular Therapy against Oxidative Stress. Molecules 2018, 23, 1826.

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