Bioconversion of Corticosterone into Corticosterone-Glucoside by Glucosyltransferase
AbstractGlucosylation of the 21-hydroxyl group of glucocorticoid changes its solubility into hydrophilicity from hydrophobicity and, as with glucocorticoid glucuronides as a moving object in vivo, it is conceivable that it exhibits the same behavior. Therefore, glucosylation to the 21-hydroxyl group while maintaining the 11β-hydroxyl group is particularly important, and glucosylation of corticosterone was confirmed by high-resolution mass spectrometry and 1D (1H and 13C) and 2D (COSY, ROESY, HSQC-DEPT and HMBC) NMR. Moreover, the difference in bioactivity between corticosterone and corticosterone 21-glucoside was investigated in vitro. Corticosterone 21-glucoside showed greater neuroprotective effects against H2O2-induced cell death and reactive oxygen species (ROS) compared with corticosterone. These results for the first time demonstrate that bioconversion of corticosterone through the region-selective glucosylation of a novel compound can present structural potential for developing new neuroprotective agents. View Full-Text
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Yamaguchi, T.; Lee, J.-H.; Lim, A.-R.; Sim, J.-S.; Yu, E.-J.; Oh, T.-J. Bioconversion of Corticosterone into Corticosterone-Glucoside by Glucosyltransferase. Molecules 2018, 23, 1783.
Yamaguchi T, Lee J-H, Lim A-R, Sim J-S, Yu E-J, Oh T-J. Bioconversion of Corticosterone into Corticosterone-Glucoside by Glucosyltransferase. Molecules. 2018; 23(7):1783.Chicago/Turabian Style
Yamaguchi, Tokutaro; Lee, Joo-Ho; Lim, A-Rang; Sim, Joon-Soo; Yu, Eun-Ji; Oh, Tae-Jin. 2018. "Bioconversion of Corticosterone into Corticosterone-Glucoside by Glucosyltransferase." Molecules 23, no. 7: 1783.
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