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Molecules 2018, 23(7), 1663; https://doi.org/10.3390/molecules23071663

Antiadipogenic Effects of Loganic Acid in 3T3-L1 Preadipocytes and Ovariectomized Mice

1
Department of Medical Genetics, Ajou University School of Medicine, Suwon 16499, Korea
2
Department of Biomedical Sciences, Ajou University Graduate School of Medicine, Suwon 16499, Korea
3
Nine B Company, Daejeon 34121, Korea
4
International Biological Material Research Center, Korea Research Institute of Bioscience & Biotechnology (KRIBB), Daejeon 34141, Korea
5
Institute of Medicinal Plants, Yunnan Academy of Agricultural Sciences, Kunming 650200, China
6
Natural Products Research Institute, Gyeonggi Institute of Science & Technology Promotion, Suwon 16229, Korea
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Received: 30 April 2018 / Revised: 27 June 2018 / Accepted: 6 July 2018 / Published: 9 July 2018
(This article belongs to the Collection Herbal Medicine Research)
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Abstract

Obesity is caused by an excess storage of body fat, resulting from a chronic imbalance between energy intake and expenditure. Gentiana lutea L. (GL) root has been reported to reduce lipid accumulation in the aortic wall of diabetic rats. Here, we performed fractionation and isolation of the bioactive constituent(s) that may be responsible for the antiadipogenic effects of the GL root extract. A single compound, loganic acid, was identified as a candidate component in the 30% ethanol extract of GL. Loganic acid treatment significantly decreased the adipocyte differentiation of 3T3-L1 preadipocytes in a dose-dependent manner. The expression of key adipogenesis-related genes such as adiponectin (Adipoq), peroxisome proliferator-activated receptor gamma (Pparg), lipoprotein lipase (Lpl), perilipin1 (Plin1), fatty acid binding protein 4 (Fabp4), glucose transporter type 4 (Slc2a4), CCAAT/enhancer-binding protein alpha (Cebpa), and tumor necrosis factor-alpha (Tnf) were significantly reduced following treatment with loganic acid. In vivo experiments in an ovariectomy-induced obesity mouse model showed that loganic acid (oral administration with 10 and 50 mg/kg/day) significantly inhibited body weight gain, total fat increase, fatty hepatocyte deposition in the liver, and adipocyte enlargement in the abdominal visceral fat tissues. These results suggest that loganic acid in the GL root extract has antiadipogenic effects in vitro and in vivo. Loganic acid may be beneficial for the prevention and treatment of obesity, particularly in menopausal obese women. View Full-Text
Keywords: obesity; adipogenesis; Gentiana lutea L.; loganic acid; 3T3-L1 cell; ovariectomized mouse obesity; adipogenesis; Gentiana lutea L.; loganic acid; 3T3-L1 cell; ovariectomized mouse
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Park, E.; Kim, J.; Yeo, S.; Kim, G.; Ko, E.-H.; Lee, S.W.; Li, W.Y.; Choi, C.W.; Jeong, S.-Y. Antiadipogenic Effects of Loganic Acid in 3T3-L1 Preadipocytes and Ovariectomized Mice. Molecules 2018, 23, 1663.

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