Comprehensive Metabolomics Analysis of Xueshuan Xinmaining Tablet in Blood Stasis Model Rats Using UPLC-Q/TOF-MS
AbstractBlood stasis syndrome (BSS) is one of the most common Chinese medicine patterns in coronary heart disease. Our previous work proved that Xueshuan Xinmaining Tablet (XXT) could treat blood stasis through regulating the expression of F13a1, Car1 and Tbxa2r. In the current study, the effect and mechanism of XXT on BSS was comprehensively and holistically investigated based on a metabolomics approach. Urine and plasma samples of 10 BBS rats treated with XXT (XT), 9 BSS model rats (BM) and 11 normal control (NC) rats were collected and then determined by UPLC-Q/TOP-MS. Multivariate analyses were applied to distinguish differentiate urinary and plasma metabolite patterns between three groups. Results showed that a clear separation of three groups was achieved. XT group was located between BM group and NC group, and showing a tendency of recovering to NC group, which was consistent with the results of hemorheological studies. Some significantly changed metabolites like cortexolone, 3α,21-dihydroxy-5β-pregnane-11,20-dione and 19S-hete and leukotriene A4, chiefly involved in steroid hormone biosynthesis, arachidonic acid metabolism and lipid metabolism, were found and identified to explain the mechanism. These potential markers and their corresponding pathways will help explain the mechanism of BSS and XXT treatment. This work also proves that metabolomics is effective in traditional Chinese medicinal research. View Full-Text
- Supplementary File 1:
PDF-Document (PDF, 655 KB)
Share & Cite This Article
Tan, J.; Wang, C.; Zhu, H.; Zhou, B.; Xiong, L.; Wang, F.; Li, P.; Liu, J. Comprehensive Metabolomics Analysis of Xueshuan Xinmaining Tablet in Blood Stasis Model Rats Using UPLC-Q/TOF-MS. Molecules 2018, 23, 1650.
Tan J, Wang C, Zhu H, Zhou B, Xiong L, Wang F, Li P, Liu J. Comprehensive Metabolomics Analysis of Xueshuan Xinmaining Tablet in Blood Stasis Model Rats Using UPLC-Q/TOF-MS. Molecules. 2018; 23(7):1650.Chicago/Turabian Style
Tan, Jing; Wang, Cuizhu; Zhu, Hailin; Zhou, Baisong; Xiong, Lingxin; Wang, Fang; Li, Pingya; Liu, Jinping. 2018. "Comprehensive Metabolomics Analysis of Xueshuan Xinmaining Tablet in Blood Stasis Model Rats Using UPLC-Q/TOF-MS." Molecules 23, no. 7: 1650.
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.