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Molecules 2018, 23(6), 1495; https://doi.org/10.3390/molecules23061495

Evaluation of the Effects of Isolated Lignin on Cellulose Enzymatic Hydrolysis of Corn Stover Pretreatment by NaOH Combined with Ozone

1
College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China
2
Bio-Pharmaceutical College, Beijing City University, Beijing 100094, China
*
Author to whom correspondence should be addressed.
Received: 30 May 2018 / Revised: 16 June 2018 / Accepted: 17 June 2018 / Published: 20 June 2018
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Abstract

In this experiment, corn stover was treated with optimal combined pretreatment conditions: 2% NaOH at 80 °C treated 2 h combined with initial pH 9 at the ozone concentration of 78 mg/mL treated 25 min. The effect of lignin removal rate on the enzymatic hydrolysis degree of cellulose during the treatment process was studied. At the same time, the lignin in the optimal pretreated corn stover was separated and extracted by enzymatic acidolysis, and its structure and connection were characterized. The results showed that the alkali combined with ozone pretreatment improved the enzymatic hydrolysis degree of the cellulose while exfoliating and degrading the macromolecular lignin into small molecules. The stable crosslink structure of the lignin-cellulose-hemicellulose was destroyed, and the lignocellulosic structure changed in favor of the enzymatic hydrolysis of the cellulose. View Full-Text
Keywords: alkaline combined with ozone; pretreatment; enzymatic hydrolysis; lignin alkaline combined with ozone; pretreatment; enzymatic hydrolysis; lignin
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Fang, S.; Wang, W.; Tong, S.; Zhang, C.; Liu, P. Evaluation of the Effects of Isolated Lignin on Cellulose Enzymatic Hydrolysis of Corn Stover Pretreatment by NaOH Combined with Ozone. Molecules 2018, 23, 1495.

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