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Molecules 2018, 23(6), 1384; https://doi.org/10.3390/molecules23061384

Comparative Proteomic Analysis of Rana chensinensis Oviduct

1
Practice Innovations Center, Changchun University of Chinese Medicine, Changchun 130117, China
2
School of Clinical Medicine, Changchun University of Chinese Medicine, Changchun 130117, China
3
Jilin Science Service Center, Changchun 130021, China
4
Jilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun 130117, China
5
College of Pharmacy, Changchun University of Chinese Medicine, Changchun 130117, China
*
Authors to whom correspondence should be addressed.
Received: 8 March 2018 / Revised: 31 May 2018 / Accepted: 5 June 2018 / Published: 8 June 2018
(This article belongs to the Special Issue Mass Spectrometric Proteomics)
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Abstract

As one of most important traditional Chinese medicine resources, the oviduct of female Rana chensinensis (Chinese brown frog) was widely used in the treatment of asthenia after sickness or delivery, deficiency in vigor, palpitation, and insomnia. Unlike other vertebrates, the oviduct of Rana chensinensis oviduct significantly expands during prehibernation, in contrast to the breeding period. To explain this phenomenon at the molecular level, the protein expression profiles of Rana chensinensis oviduct during the breeding period and prehibernation were observed using isobaric tags for relative and absolute quantitation (iTRAQ) technique. Then, all identified proteins were used to obtain gene ontology (GO) annotation. Ultimately, KEGG (Kyoto Encyclopedia of Genes and Genomes) enrichment analysis was performed to predict the pathway on differentially expressed proteins (DEPs). A total of 4479 proteins were identified, and 312 of them presented different expression profiling between prehibernation and breeding period. Compared with prehibernation group, 86 proteins were upregulated, and 226 proteins were downregulated in breeding period. After KEGG enrichment analysis, 163 DEPs were involved in 6 pathways, which were lysosome, RNA transport, glycosaminoglycan degradation, extracellular matrix (ECM)–receptor interaction, metabolic pathways and focal adhesion. This is the first report on the protein profiling of Rana chensinensis oviduct during the breeding period and prehibernation. Results show that this distinctive physiological phenomenon of Rana chensinensis oviduct was mainly involved in ECM–receptor interaction, metabolic pathways, and focal adhesion. View Full-Text
Keywords: iTRAQ; proteomics; Rana chensinensis oviduct; regulation; differentially expressed proteins iTRAQ; proteomics; Rana chensinensis oviduct; regulation; differentially expressed proteins
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Su, H.; Zhang, H.; Wei, X.; Pan, D.; Jing, L.; Zhao, D.; Zhao, Y.; Qi, B. Comparative Proteomic Analysis of Rana chensinensis Oviduct. Molecules 2018, 23, 1384.

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