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Molecules 2018, 23(3), 576; https://doi.org/10.3390/molecules23030576

GC-MS Analysis of the Composition of the Essential Oil from Dendranthema indicum Var. Aromaticum Using Three Extraction Methods and Two Columns

1
School of Traditional Chinese MateriaMedica, Shenyang Pharmaceutical University, Shenyang 110016, China
2
Fushun Drug Inspection and Testing Center, Fushun 113006, China
*
Authors to whom correspondence should be addressed.
Received: 24 January 2018 / Revised: 23 February 2018 / Accepted: 23 February 2018 / Published: 4 March 2018
(This article belongs to the Collection Recent Advances in Flavors and Fragrances)
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Abstract

Dendranthema indicum var. aromaticum, which is an aromatic plant with a strong and special fragrance throughout the whole plant, is used for the treatment of colds and headaches, and as a mosquito repellant in Shennongjia, Hubei province, China. To analyze the composition of the essential oil from this medicinal herb, we developed a gas chromatography-mass Spectrometry (GC-MS) method including microwave-assisted extraction, hydrodistillation and direct headspace analysis in two different stationary phase columns. In total, 115 volatile compounds were identified, of which 90 compounds were identified using Rxi-5MS and 78 using HP-INNOWAX. Our results revealed that the oil was mainly composed of five categories of compound: oxygenated monoterpenes (28.76–78.10%), oxygenated sesquiterpenes (4.27–38.06%), sesquiterpenes (3.22–11.57%), fatty hydrocarbons (1.65–9.81%) and monoterpenes (0–3.32%). The major constituents are α-thujone, β-thujone, cis-sabinol, sabinyl acetate and (-)-neointermedeol.However, the essential oil composition in the published literature differs significantly. Therefore, a cluster analysis was carried out using the top ten compositions in the reported literature as well as this study, using Minitab software. To provide detailed information on plant origin, the ITS1-5.8s-ITS2 region was amplified and sequenced (Accession No. MF668250). Besides, in order to provide a macroscopic view of the chemical composition, the biosynthetic pathway of the main components was summarized according to the Kyoto Encyclopedia of Genes and Genomes (KEGG) database and the published literatures. View Full-Text
Keywords: Dendranthema indicum var. aromaticum; essential oil composition; GC-MS; ITS1-5.8s-ITS2; biosynthetic pathway Dendranthema indicum var. aromaticum; essential oil composition; GC-MS; ITS1-5.8s-ITS2; biosynthetic pathway
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Fan, S.; Chang, J.; Zong, Y.; Hu, G.; Jia, J. GC-MS Analysis of the Composition of the Essential Oil from Dendranthema indicum Var. Aromaticum Using Three Extraction Methods and Two Columns. Molecules 2018, 23, 576.

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