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Molecules 2018, 23(1), 237; https://doi.org/10.3390/molecules23010237

HnRNPA1 Specifically Recognizes the Base of Nucleotide at the Loop of RNA G-Quadruplex

Division of Chemistry, Department of Medical Sciences, Faculty of Medicine, University of Miyazaki, 5200 Kihara, Kiyotake, Miyazaki 889-1692, Japan
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Received: 13 December 2017 / Revised: 5 January 2018 / Accepted: 16 January 2018 / Published: 22 January 2018
(This article belongs to the Special Issue G-Quadruplex Ligands and Cancer)
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Abstract

Human telomere RNA performs various cellular functions, such as telomere length regulation, heterochromatin formation, and end protection. We recently demonstrated that the loops in the RNA G-quadruplex are important in the interaction of telomere RNA with heterogeneous nuclear ribonucleoprotein A1 (hnRNPA1). Here, we report on a detailed analysis of hnRNPA1 binding to telomere RNA G-quadruplexes with a group of loop variants using an electrophoretic mobility shift assay (EMSA) and circular dichroism (CD) spectroscopy. We found that the hnRNPA1 binds to RNA G-quadruplexes with the 2’-O-methyl and DNA loops, but fails to bind with the abasic RNA and DNA loops. These results suggested that hnRNPA1 binds to the loop of the RNA G-quadruplex by recognizing the base of the loop’s nucleotides. The observation provides the first evidence that the base of the loop’s nucleotides is a key factor for hnRNPA1 specifically recognizing the RNA G-quadruplex. View Full-Text
Keywords: hnRNPA1; RNA G-quadruplex; telomere RNA; loop of RNA G-quadruplex; base hnRNPA1; RNA G-quadruplex; telomere RNA; loop of RNA G-quadruplex; base
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Liu, X.; Xu, Y. HnRNPA1 Specifically Recognizes the Base of Nucleotide at the Loop of RNA G-Quadruplex. Molecules 2018, 23, 237.

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