Molecules 2018, 23(1), 23; doi:10.3390/molecules23010023
Analysis of Chemical Variations between Crude and Salt-Processed Anemarrhenae rhizoma Using Ultra-High-Performance Liquid Chromatography–Mass Spectrometry Methods
De Ji 1,2,3,*
, Xiaonan Su 1,2,3, Ziyan Huang 1,2,3, Lialin Su 1,2,3, Lin Li 1,2,3,*
and Tulin Lu 1,2,3
, Xiaonan Su 1,2,3, Ziyan Huang 1,2,3, Lialin Su 1,2,3, Lin Li 1,2,3,*
and Tulin Lu 1,2,31
College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China
2
State Key Laboratory Cultivation Base for TCM Quality and Efficacy, Nanjing University of Chinese Medicine, Nanjing 210023, China
3
Key Research Laboratory of Chinese Medicine Processing of Jiangsu Province, Nanjing University of Chinese Medicine, Nanjing 210023, China
*
Authors to whom correspondence should be addressed.
Received: 17 November 2017 / Revised: 16 December 2017 / Accepted: 18 December 2017 / Published: 22 December 2017
(This article belongs to the Section Analytical Chemistry)
Abstract
The present study was designed to systematically investigate the chemical profile differences between crude Anemarrhenae rhizoma (CAR) and salt-processed Anemarrhenae rhizoma (SAR). Ultra-high-performance liquid chromatography–quadrupole time-of-flight mass spectrometry (UHPLC–QTOF-MS), coupled with multivariate statistical analysis was used for the discrimination of chemical profiles and the identification of the differentiation of the chemical constitutions of CAR and SAR. In addition, seven main constituents of CAR and SAR were simultaneously determined by ultra-high-performance liquid chromatography–quadrupole mass spectrometry (UHPLC–MS) for analyzing the content variations. A total of 24 components were found to be the main contributors to the significant difference between CAR and SAR. The structures of the marker compounds were identified based on their chromatographic behaviors, intact precursor ions, and characteristic MS fragmentation patterns. The potential structural transformation mechanism of furostanol saponins during salt processing was explored. The results may provide a scientific foundation for deeply elucidating the processing mechanism of Anemarrhenae rhizoma. View Full-TextKeywords:
Anemarrhenae rhizoma; chemical profile; UPLC–QTOF-MS; salt processing; furostanol saponins
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Ji, D.; Su, X.; Huang, Z.; Su, L.; Li, L.; Lu, T. Analysis of Chemical Variations between Crude and Salt-Processed Anemarrhenae rhizoma Using Ultra-High-Performance Liquid Chromatography–Mass Spectrometry Methods. Molecules 2018, 23, 23.
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