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Molecules 2018, 23(1), 16; doi:10.3390/molecules23010016

Characterization of Polyphenolic Content in the Aquatic Plants Ruppia cirrhosa and Ruppia maritima —A Source of Nutritional Natural Products

1
Department of Chemistry, University of Bergen, Allégt. 41, N-5007 Bergen, Norway
2
Department of Geography, University of Bergen, Fosswinckelsft. 6, N-5020 Bergen, Norway
*
Author to whom correspondence should be addressed.
Received: 10 November 2017 / Revised: 18 December 2017 / Accepted: 20 December 2017 / Published: 22 December 2017
(This article belongs to the Section Natural Products)
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Abstract

Herein, the polyphenolic content in extracts of Ruppia cirrhosa (Petagna) Grande and Ruppia maritima L.was fully characterized for the first time. High amounts of the main compound chicoric acid (CA) (≤30.2 ± 4.3 mg/g) were found in both Ruppia species. In addition, eight flavonoids, namely the 3-O-glucopyranosides and 3-O-galactopyranosides, as well as malonylated 3-O-glycosides of quercetin and isorhamnetin, were isolated and identified. The antioxidant activity of Ruppia cirrhosa extracts and isolated compounds was investigated spectrophotometrically by a 1,1-diphenyl-2-picrylhydrazyl (DPPH·) radical scavenging assay. IC50 values were 31.8–175.7 μg/mL for Ruppia cirrhosa extracts and 12.1–88.4 μg/mL for isolated flavonoids. Both individual and total phenolic and flavonoid content were quantified in crude extracts using analytical HPLC. The relative high amount of total flavonoids ranged from 5.9 to 14.7 mg/g in both species, with concentrations of individual flavonoids ranging from 0.4 to 2.9 mg/g dry weight. The content of chicoric acid was twofold more in Ruppia maritima than in Ruppia cirrhosa. Seasonal variation of the quantitative content in Ruppia cirrhosa was examined. Total flavonoid content ranged from 8.4 mg/g in October to 14.7 mg/g in August, whereas the highest concentration of chicoric acid was observed in March (29.2 mg/g). View Full-Text
Keywords: Ruppiaceae; chicoric acid; flavonoids; NMR characterization; quantification; antioxidant assay Ruppiaceae; chicoric acid; flavonoids; NMR characterization; quantification; antioxidant assay
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Hasle Enerstvedt, K.; Lundberg, A.; Jordheim, M. Characterization of Polyphenolic Content in the Aquatic Plants Ruppia cirrhosa and Ruppia maritima —A Source of Nutritional Natural Products. Molecules 2018, 23, 16.

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