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Molecules 2018, 23(1), 114; doi:10.3390/molecules23010114

Characterization of New Bioactive Enzyme Inhibitors from Endophytic Bacillus amyloliquefaciens RWL-1

School of Applied Biosciences, Kyungpook National University, Daegu 41566, Korea
UoN Chair of Oman’s Medicinal Plants & Marine Natural Products, University of Nizwa, Nizwa 616, Oman
Department of Chemistry, University of Sargodha, Sub-Campus Mianwali, Mianwali 42200, Pakistan
School of Agricultural Civil & Bio-Industrial Machinery Engineering, Kyungpook National University, Daegu 41566, Korea
Author to whom correspondence should be addressed.
Received: 24 November 2017 / Revised: 3 January 2018 / Accepted: 4 January 2018 / Published: 5 January 2018
(This article belongs to the Section Metabolites)
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Endophytic bacteria are known to produce a wide array of bioactive secondary metabolites with beneficial effects on human health. In the current study, a novel endophytic bacterial strain, Bacillus amyloliquefaciens RWL-1, was isolated from the seeds of Oryza sativa. Initially, the crude extract of RWL-1 was assessed for potential biological effects of enzyme inhibition and cytotoxicity and was found to exhibit a broad spectrum inhibition for α-glucosidase (37 ± 0.09%) and urease (49.4 ± 0.53%). The screening results were followed by bioassay-guided isolation of secondary metabolite(s) from RWL-1. Extensive chromatographic and spectrophotometry analyses revealed the presence of compound 1 (S)-2-hydroxy-N-((S)-1-((S)-8-hydroxy-1-oxoisochroman-3-yl)-3-methylbutyl)-2-((S)-5-oxo-2,5-dihydrofuran-2-yl)acetamide. Further bioassays of compound 1 showed significant inhibition of α-glucosidase (52.98 ± 0.8%) and urease (51.27 ± 1.0%), compared with positive control values of 79.14 ± 1.9% and 88.24 ± 2.2%, and negative controls (0.08 ± 0.1% and 0.05 ± 0.01%), respectively. The current study suggests that bacterial endophytes are a rich source of novel bioactive compounds with high therapeutic value. View Full-Text
Keywords: endophytes; biological potential; secondary metabolites; α-glucosidase; urease endophytes; biological potential; secondary metabolites; α-glucosidase; urease

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Shahzad, R.; Latif Khan, A.; Ali, L.; Bilal, S.; Imran, M.; Choi, K.-S.; Al-Harrasi, A.; Lee, I.-J. Characterization of New Bioactive Enzyme Inhibitors from Endophytic Bacillus amyloliquefaciens RWL-1. Molecules 2018, 23, 114.

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