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Molecules 2017, 22(9), 1489; doi:10.3390/molecules22091489

Quantitative Analysis, Extraction Optimization, and Biological Evaluation of Cudrania tricuspidata Leaf and Fruit Extracts

Department of Pharmacy, College of Pharmacy, Mokpo National University, Muan-gun, Jeonnam 58554, Korea
Laboratory of Toxicology, College of Pharmacy, Chosun University, Dong-gu, Gwangju 61452, Korea
Department of Nursing, Dongshin University, Naju-si, Jeonnam 58245, Korea
Department of Business Administration, Mokpo National University, Muan-gun, Jeonnam 58554, Korea
Department of Manufacturing Pharmacy, College of Pharmacy, Pusan National University, Geumjeong-gu, Busan 46241, Korea
These authors contributed equally to this work.
Authors to whom correspondence should be addressed.
Received: 22 August 2017 / Accepted: 5 September 2017 / Published: 7 September 2017
(This article belongs to the Section Natural Products)
View Full-Text   |   Download PDF [1765 KB, uploaded 7 September 2017]   |  


Cudrania tricuspidata Bureau (Moraceae) shows numerous pharmacological effects and has been used in traditional herbal remedies for inflammation, gastritis, tumors, and liver diseases. However, no validated analytical method for the standardization and optimization of the biological properties of C. tricuspidata preparations has been reported. We developed and validated a reverse-phase high-performance liquid chromatography (HPLC) method for the separation and quantification of active markers. Ethanolic extracts of C. tricuspidata leaves were prepared and evaluated for chemical profiles and biological activities. The 80% ethanolic extract demonstrated the greatest antioxidant activity and phenolic content, while the 100% ethanolic extract had the greatest total flavonoid content and xanthine oxidase (XO) inhibitory activity. The validated HPLC method confirmed that chlorogenic acid, rutin, and kaempferol were present in C. tricuspidata leaf extracts. We postulated that the antioxidant and anti-hyperuricemic/gout effects of C. tricuspidata extract could be attributed to these marker compounds. Our results suggested that the flavonoid-rich fraction of the leaf extract may be utilized for the treatment and prevention of hyperuricemia-related diseases, and the validated method and marker compounds could be applied for the quality control of C. tricuspidata preparations. View Full-Text
Keywords: C. tricuspidata Bureau; simultaneous analysis; HPLC; xanthine oxidase; hyperuricemia C. tricuspidata Bureau; simultaneous analysis; HPLC; xanthine oxidase; hyperuricemia

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Song, S.-H.; Ki, S.H.; Park, D.-H.; Moon, H.-S.; Lee, C.-D.; Yoon, I.-S.; Cho, S.-S. Quantitative Analysis, Extraction Optimization, and Biological Evaluation of Cudrania tricuspidata Leaf and Fruit Extracts. Molecules 2017, 22, 1489.

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