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Molecules 2017, 22(9), 1421; doi:10.3390/molecules22091421

Sensitive and Selective Detection of Oxo-Form Organophosphorus Pesticides Based on CdSe/ZnS Quantum Dots

1
State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macau 999078, China
2
State Key Laboratory of Hydraulics and Mountain River Engineering, Sichuan University, Chengdu 610000, Sichuan, China
3
School of Chemistry and Chemical Engineering, Chongqing University, Chongqing 400015, China
*
Authors to whom correspondence should be addressed.
Received: 9 August 2017 / Revised: 25 August 2017 / Accepted: 25 August 2017 / Published: 28 August 2017
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Abstract

A rapid, sensitive and enzyme-based optical biosensor was applied for the determination of seven organophosphorus pesticides (OPPs), including the oxo forms (malaoxon, paraoxon, dibrom, and dichlorvos), the thio forms (malathion and parathion) and the mixed form (demeton) in Panax ginseng. The principal of the proposed method is that the fluorescence quenching effect of quantum dots (QDs) can be observed by enzyme-generated H2O2. The active centers of acetylcholinesterase (AChE) could be inhibited in the presence of pesticides, which caused decrease of the generated H2O2. Then, the inhibition efficiency of pesticide to AChE activity could be evaluated by measuring the fluorescence changes. Different from biosensors based on immobilized enzyme or self-assembling technique, the proposed biosensor demonstrated a good selectivity for the detection of oxo forms of OPPs. In the present study, the important experimental conditions of the proposed biosensor were investigated. Under the optimized conditions (incubation temperature, 35 °C; incubation time, 20 min; pH value, 8.0; detection time, 30 min; AChE concentration, 40.9 U/L; and choline oxidase (ChOx) concentration, 637.5 U/L), the limit of detection for the investigated oxo-form OPPs was no more than 0.05 μM, which suggested that the proposed method could be used for sensitive and selective determination of trace amounts of OPPs residues in real samples with complex matrices. View Full-Text
Keywords: organophosphorus pesticide; quantum dots; acetylcholinesterase; biosensor; Panax ginseng organophosphorus pesticide; quantum dots; acetylcholinesterase; biosensor; Panax ginseng
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Wei, J.; Cao, J.; Hu, H.; Yang, Q.; Yang, F.; Wan, J.; Su, H.; He, C.; Li, P.; Wang, Y. Sensitive and Selective Detection of Oxo-Form Organophosphorus Pesticides Based on CdSe/ZnS Quantum Dots. Molecules 2017, 22, 1421.

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