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Molecules 2017, 22(9), 1396; doi:10.3390/molecules22091396

Isolation, Characterization and Bioactivities of an Extracellular Polysaccharide Produced from Streptomyces sp. MOE6

1
Department of Biochemistry, University of Missouri, Columbia, MO 65211, USA
2
Department of Microbiology and Immunology, Faculty of Pharmacy, Cairo University, Cairo 11562, Egypt
3
Department of Chemistry of Natural and Microbial Products, National Research Center, Cairo 12622, Egypt
*
Author to whom correspondence should be addressed.
Received: 8 July 2017 / Revised: 18 August 2017 / Accepted: 20 August 2017 / Published: 24 August 2017
(This article belongs to the Special Issue Advances in Natural Polysaccharides Research)
View Full-Text   |   Download PDF [3164 KB, uploaded 24 August 2017]   |  

Abstract

A Streptomyces strain was isolated from soil and the sequence of 1471 nucleotides of its 16S rDNA showed 99% identity to Streptomyces sp. HV10. This newly isolated Streptomyces strain produced an extracellular polysaccharide (EPS) composed mainly of glucose and mannose in a ratio of 1:4.1, as was characterized by Fourier transform infrared spectroscopy (FTIR), HPLC and 1H-NMR. The antioxidant activities of the partially purified MOE6-EPS were determined by measuring the hydroxyl free radical scavenging activity and the scavenging of 2,2-diphenyl-2-picryl-hydrazyl (DPPH) radicals. In addition, the partially purified MOE6-EPS showed high ferrous ion (Fe2+) chelation activity which is another antioxidant activity. Interestingly, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays that were colorimetric assays for NAD(P)H-dependent cellular oxidoreductases and a proxy of the number of viable cells, showed that the partially purified MOE6-EPS inhibited the proliferation of the human breast cancer cells (MDA-MB-231). The scratch wound assay showed that MOE6-EPS reduced the migration of mouse breast cancer cells (4T1). This study reports the production of EPS from Streptomyces species with promising antioxidant, metal chelating and mammalian cell inhibitory activities. View Full-Text
Keywords: Streptomyces; polysaccharides; antioxidant activities; tumor inhibition Streptomyces; polysaccharides; antioxidant activities; tumor inhibition
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Elnahas, M.O.; Amin, M.A.; Hussein, M.M.D.; Shanbhag, V.C.; Ali, A.E.; Wall, J.D. Isolation, Characterization and Bioactivities of an Extracellular Polysaccharide Produced from Streptomyces sp. MOE6. Molecules 2017, 22, 1396.

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