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Molecules 2017, 22(9), 1394; doi:10.3390/molecules22091394

Development and Validation of HPLC-DAD and UHPLC-DAD Methods for the Simultaneous Determination of Guanylhydrazone Derivatives Employing a Factorial Design

1
Drug Quality Control Laboratory (LCQMed), Pharmaceutical Sciences Department, Federal University of Rio Grande do Norte–UFRN, Av. General Cordeiro de Faria s/n, Natal RN 59012-570, Brazil
2
Laboratory of Medicinal Chemistry, Nursing and Pharmacy School, Federal University of Alagoas, ESENFAR, Av. Lourival de Mello Motta s/n, Maceió AL 57072-970, Brazil
3
Pharmaceutical Sciences Department, Federal University of Amapá–UNIFAP, Rod Juscelino Kubitschek, Km-02, Macapá AP 68903-419, Brazil
*
Author to whom correspondence should be addressed.
Received: 20 July 2017 / Accepted: 18 August 2017 / Published: 30 August 2017
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Abstract

Guanylhydrazones are molecules with great pharmacological potential in various therapeutic areas, including antitumoral activity. Factorial design is an excellent tool in the optimization of a chromatographic method, because it is possible quickly change factors such as temperature, mobile phase composition, mobile phase pH, column length, among others to establish the optimal conditions of analysis. The aim of the present work was to develop and validate a HPLC and UHPLC methods for the simultaneous determination of guanylhydrazones with anticancer activity employing experimental design. Precise, exact, linear and robust HPLC and UHPLC methods were developed and validated for the simultaneous quantification of the guanylhydrazones LQM10, LQM14, and LQM17. The UHPLC method was more economic, with a four times less solvent consumption, and 20 times less injection volume, what allowed better column performance. Comparing the empirical approach employed in the HPLC method development to the DoE approach employed in the UHPLC method development, we can conclude that the factorial design made the method development faster, more practical and rational. This resulted in methods that can be employed in the analysis, evaluation and quality control of these new synthetic guanylhydrazones. View Full-Text
Keywords: guanylhydrazones; factorial design; HPLC-DAD; UHPLC-DAD; method validation guanylhydrazones; factorial design; HPLC-DAD; UHPLC-DAD; method validation
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Azevedo de Brito, W.; Gomes Dantas, M.; Andrade Nogueira, F.H.; Ferreira da Silva-Júnior, E.; Xavier de Araújo-Júnior, J.; Aquino, T.M.; Adélia Nogueira Ribeiro, Ê.; da Silva Solon, L.G.; Soares Aragão, C.F.; Barreto Gomes, A.P. Development and Validation of HPLC-DAD and UHPLC-DAD Methods for the Simultaneous Determination of Guanylhydrazone Derivatives Employing a Factorial Design. Molecules 2017, 22, 1394.

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