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Molecules 2017, 22(8), 1264; doi:10.3390/molecules22081264

Screening Hepatotoxic Components in Euodia rutaecarpa by UHPLC-QTOF/MS Based on the Spectrum-Toxicity Relationship

1
Key Lab of Modern Preparations of TCM, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China
2
Jiangxi Province Center for Disease Control and Prevention, Nanchang 330004, China
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 24 June 2017 / Revised: 19 July 2017 / Accepted: 24 July 2017 / Published: 27 July 2017
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Abstract

Euodia rutaecarpa is a common traditional Chinese medicine (TCM) in clinical practice, having the ability to suppress pain and cease coughing; however, with the increasing reports showing that it is toxic, particularly hepatotoxic, the concerns raised by what cause its toxicity is growing. In the current study, an analysis method based on the spectrum effect has been employed to screen the major hepatotoxic components in Euodia rutaecarpa so that the toxic material’s basis would be elucidated. A fingerprinting method of the Euodia rutaecarpa extracts (which were petroleum ether, chloroform, ethyl acetate, n-butanol, and water) using ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometer (UHPLC-QTOF/MS) has been developed. Orthogonal partial least squares (OPLS) was used to establish the spectrum-toxicity relationship with the levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in mice serum as evaluation indices for liver injury. The UHPLC-MS fingerprint was established and the OPLS analytical results suggested that coniferin, 1-methyl-2-undecyl-4(1H)-quinolone, 1-methyl-2-[(6Z,9Z,12E)-pentadeca triene]-4(1H)-quinolone, evocarpine, 1-methyl-2-[(Z)-7-tridecenyl]-4(1H)-quinolone, dihydroevocarpine, and 1-methyl-2-tetradecy-4-(1H)-quinolone probably associated with the hepatotoxicity of Euodia rutaecarpa. This paper offered considerable methods and insight for the fundamental research of the toxic material basis of similar toxic TCMs. View Full-Text
Keywords: Euodia rutaecarpa; spectrum-toxicity relationship; hepatotoxicity; UHPLC-Q-TOF/MS; OPLS Euodia rutaecarpa; spectrum-toxicity relationship; hepatotoxicity; UHPLC-Q-TOF/MS; OPLS
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Liang, J.; Chen, Y.; Ren, G.; Dong, W.; Shi, M.; Xiong, L.; Li, J.; Dong, J.; Li, F.; Yuan, J. Screening Hepatotoxic Components in Euodia rutaecarpa by UHPLC-QTOF/MS Based on the Spectrum-Toxicity Relationship. Molecules 2017, 22, 1264.

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