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Molecules 2017, 22(7), 1108; doi:10.3390/molecules22071108

The [Mo6Cl14]2− Cluster is Biologically Secure and Has Anti-Rotavirus Activity In Vitro

1
Departamento de Ciencias Químicas y Biológicas, Universidad Bernardo O Higgins, General Gana 1702, Santiago 8370854, Chile
2
Escuela de Tecnología Médica, Universidad Bernardo O Higgins, General Gana 1702, Santiago 8370854, Chile
3
Centro Integrativo de Biología y Química Aplicada, Universidad Bernardo O Higgins, General Gana 1702, Santiago 8370854, Chile
4
Instituto de Ciencias Biomédicas, Programa de Virología, Universidad de Chile, Avda, Independencia 1027, Independencia 8380453, Chile
5
Facultad de Ingeniería, Ciencia y Tecnología, Universidad Bernardo O Higgins, Avenida Viel 1497, Santiago 8370993, Chile
*
Author to whom correspondence should be addressed.
Received: 9 May 2017 / Revised: 26 June 2017 / Accepted: 29 June 2017 / Published: 5 July 2017
(This article belongs to the Special Issue Metal Based Drugs: Opportunities and Challenges)
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Abstract

The molybdenum cluster [Mo6Cl14]2− is a fluorescent component with potential for use in cell labelling and pharmacology. Biological safety and antiviral properties of the cluster are as yet unknown. Here, we show the effect of acute exposition of human cells and red blood cells to the molybdenum cluster and its interaction with proteins and antiviral activity in vitro. We measured cell viability of HepG2 and EA.hy926 cell lines exposed to increasing concentrations of the cluster (0.1 to 250 µM), by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) colorimetric assay. Hemolysis and morphological alterations of red blood cells, obtained from healthy donors, exposed to the cluster (10 to 200 µM) at 37 °C were analyzed. Furthermore, quenching of tryptophan residues of albumin was performed. Finally, plaque formation by rotavirus SA11 in MA104 cells treated with the cluster (100 to 300 µM) were analyzed. We found that all doses of the cluster showed similar cell viability, hemolysis, and morphology values, compared to control. Quenching of tryptophan residues of albumin suggests a protein-cluster complex formation. Finally, the cluster showed antiviral activity at 300 µM. These results indicate that the cluster [Mo6Cl14]2− could be intravenously administered in animals at therapeutic doses for further in vivo studies and might be studied as an antiviral agent. View Full-Text
Keywords: cluster; cell viability; hemolysis; red blood cells; albumin; rotavirus cluster; cell viability; hemolysis; red blood cells; albumin; rotavirus
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Rojas-Mancilla, E.; Oyarce, A.; Verdugo, V.; Morales-Verdejo, C.; Echeverria, C.; Velásquez, F.; Chnaiderman, J.; Valiente-Echeverría, F.; Ramirez-Tagle, R. The [Mo6Cl14]2− Cluster is Biologically Secure and Has Anti-Rotavirus Activity In Vitro. Molecules 2017, 22, 1108.

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