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Molecules 2017, 22(7), 1075; doi:10.3390/molecules22071075

Cell-Free Production of Pentacyclic Triterpenoid Compound Betulinic Acid from Betulin by the Engineered Saccharomyces cerevisiae

Department of Food Science and Nutrition, Zhejiang University, Hangzhou 310058, China
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Received: 1 June 2017 / Revised: 24 June 2017 / Accepted: 26 June 2017 / Published: 27 June 2017
(This article belongs to the Collection Bioactive Compounds)
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Abstract

Betulinic acid is a product of plant secondary metabolism which has shown various bioactivities. Several CYP716A subfamily genes were recently characterized encoding multifunctional oxidases capable of C-28 oxidation. CYP716A12 was identified as betulin C-28 oxidase, capable of modifying betulin. This study aimed to induce the transformation of betulin to betulinic acid by co-expressing enzymes CYP716A12 from Medicago truncatula and ATR1 from Arabidopsis thaliana in Saccharomyces cerevisiae. The microsome protein extracted from the transgenic yeast successfully catalyzed the transformation of betulin to betulinic acid. We also characterized the optimization of cell fragmentation, protein extraction method, and the conversion conditions. Response surface methodology was implemented, and the optimal yield of betulinic acid reached 18.70%. After optimization, the yield and the conversion rate of betulin were increased by 83.97% and 136.39%, respectively. These results may present insights and strategies for the sustainable production of betulinic acid in multifarious transgenic microbes. View Full-Text
Keywords: betulinic acid; betulin; CYP716A12; ATR1; co-expression betulinic acid; betulin; CYP716A12; ATR1; co-expression
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Wu, J.; Niu, Y.; Bakur, A.; Li, H.; Chen, Q. Cell-Free Production of Pentacyclic Triterpenoid Compound Betulinic Acid from Betulin by the Engineered Saccharomyces cerevisiae. Molecules 2017, 22, 1075.

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