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Molecules 2017, 22(7), 1011; doi:10.3390/molecules22071011

Studies on the Two Thymine Residues in the Catalytic Core of 10-23 DNAzyme: The Impact on the Catalysis of Their 5-Substituted Functional Groups

1
College of Life Sciences, Guizhou University, Guiyang 550025, China
2
Laboratory of Bioorganic chemistry, Beijing Institute of Pharmacology and Toxicology, Beijing 100850, China
*
Author to whom correspondence should be addressed.
Received: 26 May 2017 / Revised: 9 June 2017 / Accepted: 15 June 2017 / Published: 22 June 2017
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Abstract

In the 15-mer catalytic core of 10-23 DNAzyme, each residue contributes to the catalytic conformation differently. Here, the critically conserved T4 and the least conserved T8 were modified on their 5-position with hydroxyl, imidazolyl, and amino groups with a hydrogen-bonding ability. These external functional groups induced new interactions within the catalytic core, resulting in both negative and positive effects on the catalytic activity of 10-23 DNAzyme, and the different linkages could be used to modulate the effect of the functional groups. The conservation of T4 and T8 could be recognized at the level of the nucleobase, but at the level of the functional group, T4 is not completely conserved. Their 5-methyl groups could be modified for a better performance in terms of the DNAzyme. View Full-Text
Keywords: 10-23 DNAzyme; chemical modifications; thymine; functional group; catalytic core; observed catalytic constants 10-23 DNAzyme; chemical modifications; thymine; functional group; catalytic core; observed catalytic constants
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Li, P.; Du, S.; Li, Y.; He, J. Studies on the Two Thymine Residues in the Catalytic Core of 10-23 DNAzyme: The Impact on the Catalysis of Their 5-Substituted Functional Groups. Molecules 2017, 22, 1011.

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